A method and device for preparing a three-dimensional air electrospinning scaffold based on negative pressure collection

Abstract

The invention discloses a preparation method and device for a three-dimensional support for spinning by utilizing gas-electro spinning based on negative-pressure collection. A device comprises an alternating-current high-voltage generator, a liquid supply device, a gas liquid device, a coaxial sprayer and a negative-pressure collecting device. The coaxial sprayer is provided with a liquid outlet and a gas outlet. The gas supply device is used for conveying a spinning solution or melts to the coaxial sprayer and enabling melts to flow out of the liquid outlet. The gas liquid device can generate air flow blowing from the liquid outlet to the negative-pressure collecting device via the liquid outlet. The alternating-current high-voltage generator can form an alternating electric field between the coaxial sprayer and the negative-pressure collecting device. The negative-pressure collecting device comprises a collector, a negative-pressure pipe and a negative-pressure generator. The spinning solution or melts form solution jet flow or melt jet flow in the coaxial sprayer and the collector under effect of airflow and the alternating electric field.The preparation method and device for the three-dimensional support for spinning by utilizing gas-electro spinning based on negative-pressure collection have following beneficial effects: by adoption of spinning technology based on the negative-pressure collector, the nanofiber three-dimensional support featuring a broad range of preparation material, small fiber diameters, uniformly-distributed filament diameters, a loose structure, great thickness and superior mechanical properties is obtained and has great technological universality.

Description

technical field [0001] The invention is used in the technical field of nanofiber supports, and in particular relates to a method and device for preparing a three-dimensional air electrospinning support based on negative pressure collection. Background technique [0002] Electrospinning is an effective method for preparing nanofibers. The fiber diameter is usually between tens of nanometers and several micrometers, which is similar to the size of fibrous scaffolds such as collagen in the extracellular matrix. The use of natural polymer or synthetic polymer electrospun fibers to construct tissue engineering scaffolds can mimic the structure and even biological functions of extracellular matrix, facilitate cell adhesion, differentiation and proliferation, guide tissue regeneration and repair, and become the research of tissue engineering scaffolds hotspot. A large number of research reports have shown that electrospun nanofibrous scaffolds can provide an ideal microenvironmen...

AI Technical Summary

Problems solved by technology

However, the fibers of the scaffold are piled up by the attraction of electric charge, the arrangement is tight, and the gap between the fibers is too small, which makes it difficult for cells to grow in, and it is impossible to construct a satisfactory three-dimensional tissue.

On the other hand, in traditional DC electrospinning, the direction of the electric field force is constant. When the viscosity of the spinning solution or melt is high, the starting voltage required by the jet is high.

[0006] 2. As the thickness of the film support increases, the electric field between the nozzle and the collecting plate will gradually weaken, resulting in the continuous accumulation of surface charges on the film support. Because of the same charge, the first deposited fibers will affect the electric field distribution and affect the subsequent jet flow. Repulsion occurs, which affects the formation of the Taylor cone and the duration of spinning, reduces the spinning efficiency, and eventually leads to the termination of the electrospinning process, which limits the thickness of the scaffold that can be obtained (usually micron-scale thickness), and the real fabric of large thickness Significant electrospun three-dimensional structures remain difficult

[0007] 3. When a collector with a specific shape is used, the fibers are deposited toward the tip where the electric field strength is higher, making it difficult to form a three-dimensional structure

[0008] 4. The efficiency of traditional nozzle-type electrospinning is low, and the output of single-nozzle electrospinning is usually only 0.1-1g / h, which makes it difficult to realize large-scale and high-efficiency three-dimensional scaffold manufacturing

However, the scheme of using negative pressure to collect AC air electrospun nanofibers to prepare three-dimensional scaffolds has not been reported yet.

[0012] In summary, there is still no universal solution for preparing nanofibrous three-dimensional scaffolds with a wide range of materials, extremely small fiber diameters, uniform filament diameter distribution, fluffy structure, extremely thick, and superior mechanical properties.

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