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Method for preparing fuel ethanol by joint fermentation of synanthrin containing material and cellulosic material

A technology for cellulosic raw material and fuel ethanol, which is applied in fermentation, biofuel and other directions, can solve the problem of low ethanol concentration, and achieve the effect of simple and easy method.

Inactive Publication Date: 2016-03-02
LIAONING UNIVERSITY OF PETROLEUM AND CHEMICAL TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem that the existing cellulosic ethanol technology needs to develop special equipment for high dry matter concentration system hydrolysis and the ethanol concentration in the fermentation liquid is not high, the present invention provides a low dry matter concentration cellulosic raw material synchronous saccharification fermentation and inulin-containing Coupling co-fermentation process of raw material fermentation to produce fuel ethanol

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  • Method for preparing fuel ethanol by joint fermentation of synanthrin containing material and cellulosic material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 Fermentation of corn stalks alone

[0025] 2L of tap water, 0.4kg of steam explosion pretreated corn stalks, and 50g of yeast powder were added to a 5L fermenter to adjust the pH to 5.0. At the same time, 800mLYPD culture was prepared in a 3L Erlenmeyer flask, sterilized, inoculated with FE-B yeast, and cultured overnight.

[0026] Add 200mL cellulase to the 5L fermenter, and insert 800mL yeast cultured overnight. Simultaneous saccharification and fermentation were carried out at 42°C. At 24h, 48h, 72h and 96h, 0.2kg of steam explosion pretreated corn stalks were added, and the concentration of ethanol in the system was 8.2% after 120h.

Embodiment 2

[0027] Example 2 Preparation of ethanol by fermentation of Jerusalem artichoke flour

[0028] Take 1.2kg of Jerusalem artichoke powder, add 4L of tap water to prepare a slurry of Jerusalem artichoke powder with a dry matter concentration of 23.08%, slowly add 80g of concentrated sulfuric acid with a density of 1.84, and stir while adding until the mixture is uniform, and the obtained slurry has a pH of 0.4. Add the obtained slurry into a 10L stirred acid-resistant reactor, raise the temperature of the reaction system to 60°C and keep it for 90min. Adjust the temperature of the reactor to 50°C, then use NaOH to adjust the pH of the reaction solution to 4.5, add 0.12g of starch glucoamylase (100IU / g dry matter) and 0.12g of cellulase (10IU / g dry matter), FE-B 4g of dry yeast powder was incubated at 36°C for 48 hours. Using HPLC, the glucose concentration in the reaction solution was 0.1%, the fructose concentration was 0.08%, the total residual sugar was 0.18%, the ethanol con...

Embodiment 3

[0029] Example 3 Co-fermentation of corn stalks and Jerusalem artichoke powder

[0030] Add 2.5L of tap water, 1kg of steam explosion pretreated corn stalks, 50g of yeast powder, 200mL of cellulase into a 10L fermenter, and hydrolyze at 50°C for 48h. At this time, the glucose concentration in the enzymatic hydrolysis system was measured to be 11.5%.

[0031] Add 1.2kg of Jerusalem artichoke powder and 3L of tap water to another double-layer glass reactor (5L), slowly add 80g of concentrated sulfuric acid with a density of 1.84, stir while adding, until the mixture is uniform, and the pH of the obtained slurry is 0.4. The temperature of the reaction system was raised to 60 °C and kept for 90 min. Adjust the temperature of the reactor to 50°C, and then use NaOH to adjust the pH of the reaction solution to 4.5. At this time, the concentration of fructose in the system is 16.8%, the concentration of glucose is 3.5%, and the concentration of oligosaccharides is 1.8%.

[0032] A...

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Abstract

The invention relates to a method for preparing fuel ethanol by joint fermentation of a synanthrin containing material and a cellulosic material. The method includes steps of cellulosic material pretreatment, cellulosic material enzymolysis, synanthrin hydrolysis, mixed fermentation of the cellulosic material and non-grain starch. The cellulosic material of agricultural wastes and the like and the synanthrin containing material are fermented for ethyl alcohol production, simplicity and feasibility of the method are achieved, conflict of grains with people and conflict of fields with grains are avoided, national ethyl alcohol production policies are followed, the problem of low concentration of ethyl alcohol in a final fermentation liquor during production of the ethyl alcohol only taking the cellulosic material for fermentation is solved, and the method has a promising application prospect.

Description

technical field [0001] The invention relates to a production method of fuel ethanol, in particular to a method for preparing fuel ethanol by joint fermentation of inulin-containing raw materials and cellulose raw materials. Background technique [0002] As the world's population grows and countries become more industrialized, energy consumption continues to rise. Oil is the main resource to meet energy demand, but oil resources are limited. Scientists predict that crude oil production will drop from 25.8 billion barrels (3.525 billion tons) in 2009 to 5 billion barrels by 2050. As a renewable transportation fuel, bioenergy can effectively reduce the room temperature effect, slow down environmental pollution, and at the same time change the existing unequal oil supply and demand relationship and maintain a continuous supply, which has obvious advantages over mineral fuels. Compared with other bio-energy sources such as biodiesel, the production of fuel ethanol has already re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10
CPCY02E50/10
Inventor 姚秀清杨爽陆光
Owner LIAONING UNIVERSITY OF PETROLEUM AND CHEMICAL TECHNOLOGY