High performance liquid chromatography tandem mass spectrometry detection method of galactooligosaccharides in milk powder

Abstract

The invention relates to quantitative detection of galactooligosaccharides in milk powder, in particular to an efficient liquid chromatography tandem mass spectrometry detection method for galactooligosaccharides in milk powder. The method comprises the steps that (1) a standard solution is prepared and efficient liquid chromatography tandem mass spectrometry detection is performed, and standard spectrum diagrams of mixed standard solutions with different concentrations are obtained; (2) the standard spectrum diagrams obtained in step (1) are used for preparing concentration and chromatographic peak area relation curve charts according to raffinose, stachyose and verbascose respectively; (3) pretreatment of a milk powder sample and efficient liquid chromatography tandem mass spectrometry detection are performed; (4) the contents of the raffinose, stachyose and verbascose in the sample are determined, retention time of sample spectra obtained in the step (3) is used for determining the galactooligosaccharide kinds and the chromatographic peak areas are substituted into standard curve equations of the galactooligosaccharide kinds corresponding to the step (2), and the contents of all galactooligosaccharides in the sample are calculated. The detection method has the advantages that the pretreatment process is simple, the detection speed is high, the detection result is accurate, and the sensitivity is high.

Description

technical field [0001] The invention relates to the quantitative detection of galactooligosaccharides in milk powder, in particular to a high performance liquid chromatography tandem mass spectrometry detection method for galactooligosaccharides in milk powder. Background technique [0002] Raffinose, stachyose, and verbascose are galactooligosaccharides (Galactooligosaccharides, GOS) linked by α-1,6 glycosidic bonds. Because they are rich in soybeans, they are also often called soybean oligosaccharides. The molecular structure of raffinose is that the side of sucrose close to glucose is connected with a galactose by α-1,6 glycosidic bond, which belongs to trisaccharide; Lactose belongs to tetrasaccharide; while verbascose is galactose at the end of stachyose and then connected to a galactose by α-1,6 glycosidic bond, which belongs to pentasaccharide. [0003] Raffinose, stachyose, and verbascose have physiological functions such as promoting the growth of intestinal benefi...

AI Technical Summary

Problems solved by technology

However, the existing methods have the following defects: the detection limit of the high-performance liquid chromatography detection method is generally on the order of 1 mg / kg, and the differential detector or the evaporative light scattering detector cannot accurately characterize raffinose, stachyose, verbascose, Therefore, it is easy to cause errors and misjudgments due to the interference of impurities; in addition, due to the complex composition of milk powder samples, the samples need to be pretreated before testing. The pretreatment method of milk powder samples seriously affects the accuracy of test results. Currently, the pretreatment of milk powder samples The detection effect of the method is poor

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