A circulating type single-cell capturing and transferring chip

Abstract

A circulating type single-cell capturing and transferring chip is disclosed. The chip comprises a liquid layer and a micropump. The liquid layer comprises a circular liquid path, a liquid feeding pipe, a liquid discharging pipe, and carrier flow paths, wherein the circular liquid path comprises a plurality of microsieves used for intercepting single cells in a liquid flow flowing through the liquid path, the upstream and the downstream of the microsieves are respectively provided with a second microvalve, the liquid feeding pipe and the liquid discharging pipe are communicated with the liquid path and are respectively provided with a first microvalve, the carrier flow paths are used for providing a carrier flow passing through microsieve-distributed zones of the liquid path, and wash single cells captured by the microsieves out of the liquid path, and the micropump is used for driving the liquid flow to flow in the liquid path in a one-way and circulating manner. The captured single cells can be accurately transferred to other specific positions of the chip or to the outside of the chip from capturing positions by utilization of the chip, and therefore space position limitation is got rid of, and various kinds of sing cell culture and analysis and research can be performed.

Description

technical field [0001] The invention belongs to the field of microfluidic technology, in particular to a circulating single cell capture transfer chip. Background technique [0002] With the continuous development of biotechnology research methods, the research level of biology is deepening and expanding from cell population to single cell level. Each cell is unique in time and space. Although they may come from the same ancestor, different time and space environments determine their specific genetic expression, resulting in different biological traits, which have great impact on evolution, drug resistance, and gene expression. Such research is of great value. However, analysis methods based on cell populations often conceal the differences between different cells within the population, ignoring these small but important information. Therefore, there is an urgent need to develop single-cell-based culture and analysis methods to study the differences between individual cell...

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Problems solved by technology

In order to achieve a certain number of captured single cells, it is necessary to input a large number of cells. In this literature, the number of cells is about 10,000-25,000, and the number of cells input is high, which is difficult for some small number of cell samples. Applicable; at the same time, only 70% of the microsieves capture single cells on the chip, and the applicable cell diameter is 10-15 microns. Cells smaller than this diameter range are more sensitive to the liquid flow field and are more difficult to capture. The efficiency is lower. In order to obtain as many single cells as possible, the capture efficiency of the microsieve needs to be further improved, and it can be applied to cells with smaller diameters; in addition, in this scheme, after the cells are captured, they are isolated in the area near the capture point For culture or analysis, the captured cells cannot be transferred to other areas or exported to the chip, so the limitation of the spatial position of single cells hinders the single analysis or culture strategy and cannot adapt to various research needs

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