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Artificial insemination method of pangasius sutchi

A shark and freshwater technology, applied in the field of breeding, can solve the problems of easily scratching fish, affecting the fertilization rate, slow combination of sperm and eggs, etc., and achieve the effect of increasing the hatching rate and increasing the induced labor rate

Inactive Publication Date: 2016-04-13
NANNING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the artificial insemination of freshwater sharks has been widely carried out in farms around the world, and the fertilization rate and hatching rate have also been greatly improved, but there are still some shortcomings: (1) The temperature control during the artificial insemination process is not precise enough, generally between 25-30 degrees Celsius , Freshwater sharks are tropical fish, and their insemination process requires strict temperature control, beyond its temperature range, the combination of sperm and eggs is slow, and the combination rate is reduced, which greatly affects its fertilization rate
(2) No debonding agent is used. Freshwater shark eggs are sticky. If no debonding agent is used, the fertilized eggs will stick together, resulting in a large number of fertilized eggs being suffocated to death.
(3) The tools for catching broodstock are rough, and it is easy to scratch the fish during artificial insemination

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A kind of artificial insemination method of freshwater shark provided by the invention comprises the following steps:

[0020] (1) Select 3 4-year-old mature female freshwater sharks and 3 3-year-old mature male freshwater sharks, inject oxytocic agent at the base of the dorsal fin, the oxytocin dose is 0.5mL / L body weight, 6h after injection, the broodstock begins to estrus, and lasts 3h; The oxytocic agent is DOM;

[0021] (2) The male fish and the female fish are entwined and rolled over each other, and the water splash is turned up, indicating that when the estrus climax is reached, use the parent fish clip (made of three layers of medical waterproof gauze and bamboo sticks) to pick up the female fish, and dry the water with gauze. Then place it parallel to the top of the porcelain basin, with the belly facing the mouth of the porcelain basin (the shape of the porcelain basin is a cylinder with a height of 15-18cm and a diameter of 15-20cm on the bottom surface), an...

Embodiment 2

[0026] A kind of artificial insemination method of freshwater shark provided by the invention comprises the following steps:

[0027] (1) Select 3 4-year-old mature female freshwater sharks and 3 3-year-old mature male freshwater sharks, inject oxytocic agent at the base of the dorsal fin, the oxytocin dose is 0.5mL / L body weight, 7h after the injection, the broodstock begins to estrus, and lasts for 4h; The oxytocic agent is LHRH-A;

[0028] (2) The male fish and the female fish are entwined and rolled over each other, and the water splash is turned up, indicating that when the estrus climax is reached, use the parent fish clip (made of three layers of medical waterproof gauze and bamboo sticks) to pick up the female fish, and dry the water with gauze. Then place it parallel to the top of the porcelain basin, with the belly facing the mouth of the porcelain basin (the shape of the porcelain basin is a cylinder with a height of 15-18cm and a diameter of 15-20cm on the bottom s...

Embodiment 3

[0033] A kind of artificial insemination method of freshwater shark provided by the invention comprises the following steps:

[0034] (1) Select 3 4-year-old mature female freshwater sharks and 3 3-year-old mature male freshwater sharks, inject oxytocic agent at the base of the dorsal fin, the oxytocin dose is 0.5mL / L body weight, 6-7h after injection, the broodstock begins to estrus, and the duration 3.5h; the oxytocic agent is a mixture of HCG and DOM in equal proportions;

[0035] (2) The male fish and the female fish are entwined and rolled over each other, and the water splash is turned up, indicating that when the estrus climax is reached, use the parent fish clip (made of three layers of medical waterproof gauze and bamboo sticks) to pick up the female fish, and dry the water with gauze. Then place it parallel to the top of the porcelain basin, with the belly facing the mouth of the porcelain basin (the shape of the porcelain basin is a cylinder with a height of 15-18cm...

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Abstract

The invention provides an artificial insemination method of pangasius sutchi, and belongs to the technical field of breeding. Artificial insemination is achieved by injecting odinagogue to conduct spawning induction, taking fertilized eggs and conducting insemination. When artificial insemination of pangasius sutchi is conducted through the method, parent fish clamps are manufactured through three-layer medical waterproof gauze and bamboo sticks, breeding pangasius sutchi is prevented from being scratched during artificial insemination operation, the spawning induction rate of artificial spawning induction is increased through the unique odinagogue, and the hatching rate is increased through a unique debonding agent and temperature control. Artificial spawning induction and hatching both reach a good productivity level, and it is shown through tests that when the method is used, the spawning induction rate reaches 93% or above and the hatching rate reaches 70-80%.

Description

technical field [0001] The invention belongs to the technical field of breeding and relates to an artificial insemination method for freshwater sharks. Background technique [0002] Freshwater sharks, the scientific name of Su's round-bellied fish, also called badinfish, are mainly distributed in Southeast Asia, belonging to the order Teleskeleta, Siluriformes, Ichthyaceae, and the genus Round-bellied fish. Currently, they are found in Guangdong, Hainan, Guizhou, etc. There are more breeding in southern provinces. Freshwater sharks grow fast, tolerate low oxygen, have few diseases, are easy to raise, do not require high feed, have good meat quality, no intermuscular spines, high nutritional value, and a market value of 30 yuan / kg. They also have a very high ornamental value. The outlook is promising. Moreover, freshwater sharks also have cartilage tissue similar to sea sharks, and contain chondroitin sulfate with high medicinal value. In Guangxi Zhuang Autonomous Region, ...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81A01K61/00
Inventor 陈涛
Owner NANNING UNIV