A multi-index detection microfluidic chip and detection method
A microfluidic chip, multi-index technology, applied in fluid controllers, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of waste of reagents, detection costs, diverse detection indicators, complex sample components, etc. The effect of sample and reagent consumption, reducing detection cost, and simplifying experimental operation
Active Publication Date: 2018-06-29
广州迪澳生物科技有限公司
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[0003] Due to the broad application prospects of microfluidic chip analysis technology in the field of analysis and detection, in view of the objective factors such as complex sample components, various detection indicators, various detection reagents, serious waste of reagents, and high detection costs in the current detection process, a multi-indicator method is proposed. The microfluidic core solution for detection is an urgent technical problem to be solved
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[0019] refer to Figure 1 to Figure 3 , a microfluidic chip for multi-indicator detection in the present invention, comprising a chip cover 10 and a chip bottom 20 that are integrally sealed and connected, the center of the chip cover 10 is provided with a liquid inlet channel 11, and the outside of the chip cover 10 is spaced along the circumferential direction A plurality of air outlet channels 12 are arranged and run through, a liquid inlet chamber 21 is arranged in the center of the chip bottom sheet 20, and a plurality of reaction chambers 22 are arranged at intervals along the circumferential direction in the middle of the chip bottom sheet 20, and the liquid inlet chamber 21 is connected with each reaction chamber. The cavities 22 are connected by microfluidic channels 23, and a plurality of gas chambers 24 respectively connected to each gas outlet channel 12 are arranged at intervals along the circumferential direction outside the chip substrate 20, and each gas chamber...
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Abstract
The invention discloses a multi-index detection microfluidic chip and a detection method. The microfluidic chip includes a chip cover and a chip bottom. The center of the chip cover is provided with a liquid inlet channel, and the outside of the chip cover is provided with a plurality of outlets. The air channel, the center of the chip film is provided with a liquid inlet chamber, and the middle of the chip film is provided with a plurality of reaction chambers. A plurality of gas chambers are arranged in a row, and each gas chamber is connected to each reaction chamber through a gas channel. The sample solution to be tested is dripped into the liquid inlet chamber through the liquid inlet channel, and the microfluidic chip is subjected to a centrifugal reaction. , the sample solution to be tested is evenly distributed and flows into each reaction chamber to react with the reaction primers, and the result can be obtained by checking and comparing the reaction conditions in the reaction chambers, and the operation is simple and convenient.
Description
technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to a multi-index detection microfluidic chip and a detection method. Background technique [0002] Microfluidic chip analysis technology integrates the basic operation units such as sample preparation, reaction, separation, and detection in the biological, chemical, and medical analysis processes into a micro-nano scale chip, and automatically completes the entire detection and analysis process. [0003] Due to the broad application prospects of microfluidic chip analysis technology in the field of analysis and detection, in view of the objective factors such as complex sample components, various detection indicators, various detection reagents, serious waste of reagents, and high detection costs in the current detection process, a multi-indicator method is proposed. The microfluidic core solution for detection is an urgent technical problem to be solved. Contents of th...
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IPC IPC(8): C12M1/00C12M1/34C12Q1/686
CPCB01L3/5027B01L2300/087B01L2400/0409C12Q1/686C12Q2565/629C12Q2537/143C12Q2545/101
Inventor 唐大运张璜吴三喜李婷骆康杰邓梓欣申洪杰刘美贤张心灵石磊
Owner 广州迪澳生物科技有限公司