Unlock instant, AI-driven research and patent intelligence for your innovation.
Application of endophytic fungus grph-0 in Sophora vietnamese in the control of Candida albicans
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of Candida albicans and endophytic fungi, applied in the field of microbiology, can solve difficult problems and achieve the effect of low cost and simple method
Active Publication Date: 2019-06-21
GUANGXI UNIV
View PDF1 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
At present, the drugs commonly used in clinical treatment of Candida albicans infection mainly include azoles such as fluconazole, polyenes such as amphotericin B and pyrimidines such as 5-fluorocytosine, but with the wide application of these antifungal drugs, leading to The emergence of a large number of drug-resistant strains, therefore, Candida albicans infection has become an increasingly serious problem, bringing great difficulties and challenges to clinical treatment, and there is an urgent need to develop new antifungal drugs
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0024] Isolation, screening and identification of strains
[0025] 1. Isolation of strains
[0026] Materials for testing: cultivated Sophora japonica collected from the herbarium of Guangxi University.
[0027] Medium: PDA medium: 1000ml medium contains 300g of potato, 20g of glucose, 20g of agar, 0.1g of chloramphenicol, the pH of the medium is 6.0±0.2, and it is sterilized by moist heat at 121°C for 25min.
[0028] Surface disinfection: Rinse the fresh and healthy Vietnamese Sophorae root with a length of 6-8cm and a width of 1-2cm with running water for 30 minutes to wash away the sediment, then rinse the washed Vietnamese Sophorae root with sterile water twice, and air-dry the surface moisture , moved to the ultra-clean workbench, and under aseptic conditions, the Sophora japonica root was soaked in 75% ethanol for 1 min, rinsed with sterile water twice, soaked in sodium hypochlorite (available chlorine 1%) for 2 min, washed with sterile water for 3 times , dry the surf...
Embodiment 2
[0081] Inhibitory effect of metabolites of strains on Candida albicans
[0082] 1. Fermentation of strains and extraction of metabolites
[0083] (1) Inoculate the endophytic fungus GRPH-0 of Sophora japonica in PDA medium, and place it at a temperature of 28°C for 20 days. The resulting culture material is cut into small pieces and transferred to a sterile solid medium (400g potato, 20g Dextrose and 20g sucrose) in a 2-liter Erlenmeyer flask, placed at 28°C for fermentation for 30 days;
[0084] (2) After the fermentation is completed, soak the fermented product with 2 times the methanol of the fermented product and ultrasonically for 40 minutes, then filter it with gauze;
[0085] (3) Repeat step (2) twice, combine the two filtrates and concentrate under reduced pressure to form an extract, and obtain the methanol crude extract of the endophytic fungus GRPH-0, which is the metabolite of the endophytic fungus GRPH-0 .
[0086] 2. Minimum inhibitory concentration of GRPH-0 ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses an endophytic fungus GRPH-0 of the Vietnamese Sophora japonica. The taxonomy of the Vietnamese Sophora japonica endophytic fungus GRPH-0 is named as Myrothecium sp. GRPH-0, and the ITS sequence of the strain is shown in SEQ ID NO.1 Description, preservation unit: General Microbiology Center of China Microbiological Culture Collection Management Committee, the preservation address is the Institute of Microbiology, Chinese Academy of Sciences, No. 1, No. 1 Beichen West Road, Chaoyang District, Beijing, and the preservation date: September 29, 2015. The preservation number: CGMCC No. 11391. The endophytic fungus (Myrothecium sp.) GRPH-0 of the present invention has a very significant inhibitory effect on Candida albicans; the method for preparing the metabolite of the Vietnamese Sophora japonica endophytic fungus (Myrothecium sp.) GRPH-0 is simple and easy, and the cost is low.
Description
technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to the application of an endophytic fungus GRPH-0 in the prevention and treatment of Candida albicans. Background technique [0002] Candida albicans is a common opportunistic pathogenic fungus in the human body. When the body's immunity is weakened or the immune system is damaged, it will reproduce in large numbers and cause disease. In the past 20 years, due to the clinical use of anti-tumor drugs, antibacterial agents and immunosuppressants for tumor patients and organ transplant patients, as well as the prevalence of chronic wasting diseases and AIDS, the incidence of Candida albicans infection is higher than that of immunocompromised patients. sharply increased in the population. Candida albicans infection has increasingly become a common disease, frequently-occurring disease, and the mortality rate is increasing year by year. At present, the drugs commonly used ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More