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Application of myb37 protein and its coding gene in regulating plant drought resistance

A technology encoding genes and drought resistance, which is applied in the application field of MYB37 protein and its encoding genes in regulating plant drought resistance, and can solve problems such as difficult stress tolerance traits

Inactive Publication Date: 2018-10-16
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional breeding techniques are relatively difficult to cultivate and improve stress tolerance traits, and cannot obtain more excellent drought-resistant varieties

Method used

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  • Application of myb37 protein and its coding gene in regulating plant drought resistance
  • Application of myb37 protein and its coding gene in regulating plant drought resistance
  • Application of myb37 protein and its coding gene in regulating plant drought resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1, the acquisition and identification of MYB37 transgenic plants

[0049] The MYB37 gene involved in this example is derived from Arabidopsis thaliana, and its sequence in the Arabidopsis genome is shown in sequence 1 in the sequence table, and sequence 1 consists of 1793 nucleotides, which is the The sequence of the MYB37 gene in the Arabidopsis genome, wherein the 236-327 and 458-854 positions are all intron sequences; the cDNA sequence of the MYB37 gene is shown in sequence 2 in the sequence table, and the sequence 2 consists of 1304 nucleosides The acid composition is the cDNA sequence of the MYB37 gene, wherein the 100th-1089th position is the coding sequence (ORF); both sequence 1 and sequence 2 encode the protein shown in sequence 3 in the sequence list, and sequence 3 consists of 329 amino acid residues composition.

[0050] 1. Construction of recombinant expression vector pCAMBIA-1300-221-MYB37

[0051] The total RNA of Arabidopsis wild-type (Col e...

Embodiment 2

[0086] Embodiment 2, MYB37 transgenic plant drought resistance analysis test

[0087] ABA is an important signaling molecule for plants to resist external adversity stress. Under drought conditions, ABA can promote stomatal closure to reduce water loss by regulating ion channels in stomatal guard cells. Calcium ions, protein kinases, and phosphatases are all involved in ABA-mediated guard cell signal transduction and the signal regulation of ABA-related stress resistance genes. Therefore, ABA-induced stomatal movement experiments, dehydration experiments in detached leaves and drought experiments were used to detect whether MYB37 plays a role in the process of ABA regulating plant drought stress response.

[0088] 1. ABA promotes stomatal closing and ABA inhibits stomatal opening experiment

[0089] (1) ABA-promoted stomatal closure experiment

[0090] With Arabidopsis wild type (Col-0 ecotype), two T that embodiment 1 obtains 3 The homozygous MYB37 transgenic lines OE1 an...

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Abstract

The invention discloses application of protein MYB37 and an encoding gene thereof to regulation and control over drought resistance of plants. The application is specifically application of the protein (namely the protein MYB37) composed of an amino acid sequence as indicated in sequence 3 in a sequence list to the following occasion (a1) or (a2): (a1) improvement of drought resistance of plants; (a2) breeding of plant varieties with the drought resistance improved. Compared with a wild type control plant, tolerance of an MYB37 gene overexpression plant to stress of a drought adverse environment is remarkably improved. The application of the protein MYB37 and the encoding gene thereof has a great significance for research of drought-resisting molecular mechanisms of the plants; in addition, the gene has an important function in cultivation of drought-resistant plant varieties, thereby important possibility is provided for cultivation of new crop varieties which can resist to adverse environments, and a great significance is achieved for agricultural production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of a MYB37 protein and its coding gene in regulating the drought resistance of plants. Background technique [0002] With the gradual reduction of the world's arable land and the increase of the world's population, people's demand for food is increasing. At present, the shortage of water resources has become a crisis facing the world. Among all biological and environmental adversities, drought is the second limiting factor affecting crop yield, second only to disease and pest factors. Research on crop drought resistance is of great significance to agricultural production. It is relatively difficult to cultivate and improve stress-tolerant traits by traditional breeding techniques, and it is difficult to obtain more excellent drought-resistant varieties. With the development of molecular biology technology and in-depth research on the molecular mechanism of plant stre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8273
Inventor 张大鹏于泳涛王小芳
Owner TSINGHUA UNIV