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A protein production method, system and product

A production method and production system technology, applied in the field of solid waste recycling, can solve the problems of affecting the function of protein use, no subsequent purification process, target protein decomposition, etc., to alleviate the problem of equipment corrosion, reduce energy consumption and high cost, The effect of lowering the temperature of alkaline hydrolysis

Active Publication Date: 2019-01-22
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Therefore, the above-mentioned protein release methods all have the risk of decomposing the target protein. Whether the final product is protein, its composition and properties, and whether it meets the requirements of use have not been mentioned in the existing research reports.
At the same time, in the existing reports on protein extraction from waste, most of the protein eluates are concentrated to varying degrees, which consumes a lot of energy, and there is almost no subsequent purification process. Substances, including sugars, heavy metals, etc., not only affect the function of protein, but also have certain safety hazards

Method used

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  • A protein production method, system and product
  • A protein production method, system and product
  • A protein production method, system and product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Add the remaining sludge (water content 80%) from the urban sewage treatment plant and the preheated water from the heat exchange device 500 into the storage device 100, adjust it to a mixed solution of 75g / L (dry matter), heat it to 65°C, and put the above The preheated mixed solution is pumped into the reaction equipment 201 in the hydrolysis device 200, where two-stage hydrolysis is carried out, at first adding 1:1 papain and alkaline protease, the total addition is 4%, enzymolysis 3h, and then Heat the mixed solution to 70°C, use caustic soda to adjust the pH value to 10, and hydrolyze with hot alkali for 3 hours to obtain the first protein elution solution. The temperature of the first protein elution solution is lowered through the heat exchange device 500, and then the solid-liquid separation is carried out to obtain the second protein elution solution. One clear liquid and residue; the hot water produced by heat exchange is used to prepare waste mixed liquid, and...

Embodiment 2

[0046] Add the remaining sludge (water content 80%) from the urban sewage treatment plant and the preheated water from the heat exchange device 500 into the storage device 100 together, adjust it to a mixed solution of 50g / L (dry matter), heat it to 45°C, and put the above The preheated mixed solution is pumped into the reaction equipment 201 in the hydrolysis device 200, where two-stage hydrolysis is carried out, at first adding 1:1 papain and alkaline protease, the total addition is 5%, enzymolysis 2h, and then The mixed solution is heated to 100°C, the pH value is adjusted to 12.5 with quicklime, and the first protein elution solution is obtained by hot alkali hydrolysis for 1 hour. Clear liquid and residue; the hot water produced by heat exchange is used to prepare waste mixed liquid, and the first clear liquid of the above-mentioned solid-liquid separation is pumped into the protein extraction equipment 401 in the extraction device 400, and the pH value is adjusted to 3.5 ...

Embodiment 3

[0049] Add the remaining sludge (water content 80%) from the urban sewage treatment plant and the preheated water from the heat exchange device 500 into the storage device 100, adjust it to a mixed solution of 30g / L (dry matter), heat it to 55°C, and put the above The preheated mixed solution is pumped into the reaction equipment 201 in the hydrolysis device 200, where two-stage hydrolysis is carried out, first adding 1:1 papain and α-glucosidase, the total addition is 1.5%, enzymolysis 2h, Then heat the mixed solution to 80°C, use caustic soda to adjust the pH value to 12.5, and hydrolyze with hot alkali for 1 hour to obtain the first protein elution solution, pass the heat exchange device 500 to lower the temperature of the above first protein elution solution, and then perform solid-liquid separation, Obtain the first clear liquid and residue; the hot water generated by heat exchange is used to prepare waste mixed liquid, and the above-mentioned first clear liquid of solid-l...

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Abstract

The invention discloses a production method and system for protein and the product. The method includes the steps that water is added to organic solid waste, and the organic solid waste and the water are adjusted into a 10-100 g / L mixed solution; the mixed solution is hydrolyzed, enzymes with the mass percent of 0.5% to 5% are added, enzymolysis is carried out for 1-3 h, then the mixed solution is heated to 55-100 DEG C, the pH value is adjusted to be 10-13, alkaline thermal hydrolysis is carried out for 1-3 h, cell breaking is carried out, microbial protein is digested, and a first protein digested solution is obtained; solid-liquid separation is carried out on the first protein digested solution, and a first clear solution and residues are obtained; the first clear solution settles, and the protein product and a second clear solution are obtained. The production method and system are simple in process, convenient to manage and easy to achieve.

Description

technical field [0001] The invention belongs to the technical field of solid waste recycling, and relates to a method for producing protein from organic solid waste, a used production system and corresponding protein products obtained. Background technique [0002] Solid waste refers to solid and semi-solid waste produced by humans in production, consumption, life and other activities (the definition abroad is more extensive, and waste produced by animal activities also falls into this category). In layman's terms, it is " Rubbish". According to their properties, solid waste can be divided into organic matter and inorganic matter, that is, organic solid waste and inorganic solid waste. Among them, organic solid waste has a high moisture content and is rich in organic matter such as proteins and polysaccharides that are easily biodegradable. Therefore, it is easy to spoil and deteriorate, polluting the environment. At present, the treatment methods of organic solid waste ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12M1/00
CPCC12M47/06C12N1/00
Inventor 李秀芬施正华宋小莉齐希光王新华任月萍
Owner JIANGNAN UNIV