Pseudomonas protegens S63 and its application in controlling water hyacinth
A Pseudomonas, S63 technology, applied in pest control, bacteria, biological water/sewage treatment, etc., can solve the problem of few research reports on water hyacinth pathogenic bacteria, and achieve good purification effect.
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Embodiment 1
[0018] (1) Strain screening
[0019] Take the leaves of the water hyacinth diseased plants, scrub them with hypochlorous acid solution, wash them three times with sterile water, grind them, take the juice, and spread them on the water hyacinth agar plate medium. From the water body where the water hyacinth diseased plants are located, 1 mL of water sample was taken and spread on the water hyacinth agar plate medium, and cultured in an incubator at 28° C. for 2-4 days. Pick a single colony and purify the strain, and store the purified strain on a PDA slant.
[0020] The purified strains were planted on the ammonia nitrogen plate medium, cultured at 28°C for 2-4 days, and the strains that produced transparent circles were picked and preserved.
[0021] Take a normal growing water hyacinth plant, repeatedly scrub the surface of its stem with 70% alcohol, rinse it several times with sterile water, cut a small section about 5 cm long, put it in a petri dish lined with sterile filt...
Embodiment 2
[0032] Pseudomonas (Pseudomonas protegens) S63 in the present invention is to the test of water hyacinth pathogenicity in embodiment 2
[0033] Pseudomonas protegens S63 was inoculated in the fermentation medium (same as in Example 1), and cultured at 30° C. for 48 hours to prepare a Pseudomonas protegens S63 bacterial liquid.
[0034] (1) Simulation experiment of natural ecological cultivation of water hyacinth.
[0035]Transplant 10 young plants of water hyacinth with normal growth in 8L and add 0.2-2.5% (volume proportion) Pseudomonas protegens (Pseudomonas protegens) S63 bacterium liquid in the improved Hoagland's nutrient solution, simultaneously with not adding bacterium liquid As a blank control, three groups of parallel controls were established. Cultivate in the open air, observe and record the growth of water hyacinth. After cultivating for several days, the water hyacinths cultivated in the growth solution containing Pseudomonas protegens S63 all withered to varyi...
Embodiment 3
[0050] Pseudomonas protegens (Pseudomonas protegens) S63 in embodiment 3 of the present invention is to the purification test of eutrophic water body
[0051] Pseudomonas protegens S63 was inoculated in the fermentation medium (same as in Example 1), cultured at 30° C. and 160 r / min for 48 hours to prepare a Pseudomonas protegens S63 bacterial liquid.
[0052] Take 1000mL of eutrophication water and divide it into 2000mL beakers. Insert Pseudomonas protegens (Pseudomonas protegens) S63 bacterium liquid among the present invention by the volume ratio of 1:10000, be blank control group with not adding bacterium liquid ( figure 2 CK in ) were set up in three parallel groups and cultured at 28°C. Use intermittent aeration (that is, aeration for 12h, stop aeration for 12h). After 3 days, samples were taken to determine the contents of ammonia nitrogen and COD in the water. figure 2 for the experimental group ( figure 2 S63 in ) and control group ( figure 2 CK) in the degra...
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