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A molecular marker locus linked to the color trait of pear peel and its application

A molecular marker and trait technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of low efficiency and long period of pear genetics and breeding, and achieve good application prospects and good parents. Genetic characteristics, the effect of improving efficiency

Active Publication Date: 2019-04-19
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Technical problem to be solved: In order to solve the problems of long cycle and low efficiency in pear genetic breeding, the present invention provides a locus and molecular marker linked to the color trait of pear peel and its application

Method used

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  • A molecular marker locus linked to the color trait of pear peel and its application
  • A molecular marker locus linked to the color trait of pear peel and its application
  • A molecular marker locus linked to the color trait of pear peel and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1 source of material

[0021] The test materials were 348 individual plants and 2 parental materials of the 5-year-old 'Mantianhong'×'Hongxiangsu' hybrid population colonized at the Xinxiang Experimental Base of Zhengzhou Institute of Pomology, Chinese Academy of Agricultural Sciences.

[0022] 2 methods

[0023] 2.1 Phenotype identification

[0024] The red-skinned and green-skinned phenotypic investigations were carried out on individual plants of the Tianhong × Hongxiangsu hybrid population for two consecutive years, and the survey results were used for subsequent molecular marker verification and analysis.

[0025] 2.2 PCR reaction

[0026] The extracted DNA was amplified with the In2130-16 molecular marker, and a 20 μL reaction system was used for PCR. The primer pair for amplifying the SCAR marker is shown in Table 1. The composition of the reaction system and the PCR reaction procedure are shown in Table 2 and Table 3:

[0027] Table 1 SCAR marker primer seque...

Embodiment 2

[0046] 1. Collected from 'Xingshui'×'Red Crispy', 'Zaomeisu'×'Red Crispy', 'Zhongli No. 1'×'Red Crispy' and 'New Century'×'Red Crispy' ' and other hybrid combinations totaled 33 freshly germinated young leaves of excellent lines, carefully washed and dried, and DNA was extracted.

[0047] 2. The extracted DNA was amplified with the In2130-16 molecular marker, using a 20 μL reaction system for PCR, and the primer pair for amplifying the molecular marker was the same as in Example 1.

[0048] 3. Perform polyacrylamide gel electrophoresis on the PCR product, and perform band statistics on the electrophoresis results, and the band pattern of the red-skinned / green-skinned individual plant.

[0049] 4. The results showed that the In2130-16 marker could accurately distinguish 32 of the 33 excellent lines, and the accuracy rate reached more than 96%.

[0050] 5. Through the above steps, red-skinned pears and green-skinned pears can be easily and quickly distinguished, and molecular m...

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Abstract

The invention provides a molecular marker locus linked with a pear peel color character and application thereof. An InDel locus is located at 920222bp of Dangshan pear genome assemble Scaffold NW-008988130.1, the sequence is TATTA or T, and an encoding direction is a 5'-3' direction. A genetic distance between SCAR molecular marker In2130-16 marker developed according to the InDel locus and a tested Mantianhong and Hongxiangshu group red / green peel character locus is 4.3 cm, all red peal single plants of the tested group can be selected, and the selection efficiency is up to 100%. The molecular marker locus provided by the invention can be taken as a peal red peel character marker and then is applied to breeding selection, so that the breeding selection efficiency of red-peel peals is greatly improved, and the application prospect is excellent.

Description

technical field [0001] The invention belongs to the fields of pear genetic breeding and molecular biology, and relates to a molecular marker site linked with the color trait of pear peel and its application. Background technique [0002] Pear is an international bulk fruit and also one of the main fruits in my country. Its fruit is rich in nutrition, delicious and juicy, sweet and delicious, and is deeply loved by people. my country is the main origin of oriental pear species, and the species and variety resources are very rich. Peel color is the fruit character that attracts people's attention the most, and is an important part of fruit quality and commerciality. In recent years, the concept of healthy consumption has become more and more popular. Red pears are considered to have higher health value and are favored by the market. The selection of red pear varieties is also increasingly valued by pear breeding research institutions in various countries. [0003] At present...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 薛华柏李秀根杨健王龙王苏珂苏艳丽张慧蓉
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI