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Immunochromatographic test paper strip and preparation method and application thereof

An immunochromatographic test paper and immunofluorescence technology, which is applied in the field of in vitro diagnostic reagents, can solve the problems of inability to realize multiple detection of samples and low sensitivity, and achieve the effects of reducing detection costs, simple operation, and rapid detection

Inactive Publication Date: 2016-04-27
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

The disadvantage is that the colloidal gold test strip can only qualitatively detect samples, and the sensitivity is low, and it cannot realize multiple detection of samples

Method used

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  • Immunochromatographic test paper strip and preparation method and application thereof

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Embodiment Construction

[0024] The invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0025] Preparation of joint detection test strips based on quantum dots for lung cancer markers NSE and CEA:

[0026] Step 1: Preparation of quantum dot immunofluorescence probe

[0027] Put 0.5 ml of quantum dots in 0.5 ml of MES (2-(N-morpholine)ethanesulfonic acid) solution (0.01 mol, pH 6.0), and mix well. Then add EDC and NHS to the mixed solution, react at room temperature for 30 minutes and centrifuge to obtain the quantum dots activated by surface carboxyl groups, which are respectively mixed with NSE monoclonal antibody (mouse anti-NSE monoclonal antibody L1C00502) and CEA monoclonal antibody ( Mouse anti-CEA monoclonal antibody L1C00202) was placed in 1×PBS (phosphate buffer saline, pH7.4), reacted at 17°C for 3 hours, centrifuged, and then added 1×PBS buffer containing 1.5% BSA (pH7.4 ) to block the unreacted carboxyl groups on the quantum dots, and ...

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Abstract

The present invention discloses an immunochromatographic test paper strip which includes a sample pad, a conjugate pad, a nitrocellulose membrane, testing lines, a quality control line, a water absorbent pad and a backing plate; the conjugate pad is loaded with two immunofluorescence probes using quantum dots as markers, and the quantum dots are respectively coupled with a NSE antibody and a CEA antibody to form anti-NSE immunofluorescence and anti-CEA immunofluorescence probes; and the number of the testing lines is two, one testing line includes the NSE antibody, and the other testing line includes the CEA antibody. The immunochromatographic test paper strip is simple and fast in detection, intuitive in test results, capable of qualitatively, quantitatively even semi-quantitatively detecting, and low in price, can simultaneously detect two or more tumor markers, and achieves multiple detection of the tumor markers by use of the quantum dot test paper strip for detection.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to an immunochromatographic test strip and its preparation method and application. Background technique [0002] Realizing highly sensitive detection of tumor markers in organisms is an important topic in the field of life sciences, and developing a new detection method with high sensitivity has also become the goal of researchers. The detection of serum tumor markers has attracted people's attention because of its low cost, simplicity and non-invasiveness, reproducible detection, and qualitative, semi-quantitative or quantitative observation of detection results. The production of tumor markers can be divided into two types: First, in the process of malignant tumor occurrence and proliferation, they are synthesized and secreted by the gene expression of tumor cells, exist in cells, tissues or body fluids, and can be quantified by certain methods and Substances that can ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/574G01N33/558
CPCG01N33/577G01N33/558G01N33/57423G01N33/57473G01N33/57484G01N2333/47
Inventor 王侃肖琨崔大祥
Owner SHANGHAI JIAO TONG UNIV
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