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A kind of crucian carp brain tissue cell line and application thereof

A technology of tissue cells and crucian carp brain, applied in the crucian carp brain tissue cell line and its application field, can solve the problems of limiting the in-depth research of crucian carp virus disease and disease prevention and control, and lack of

Inactive Publication Date: 2020-03-31
深圳出入境检验检疫局动植物检验检疫技术中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as mentioned above, various viruses that infect crucian carp target the brain tissue of crucian carp; the lack of relevant research and reports on crucian carp brain tissue cell lines greatly limits the in-depth research and development of crucian carp virus disease. Disease control

Method used

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  • A kind of crucian carp brain tissue cell line and application thereof
  • A kind of crucian carp brain tissue cell line and application thereof
  • A kind of crucian carp brain tissue cell line and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0027] 1. Construction and preservation of crucian carp brain tissue cell lines

[0028] 1. Tissue Isolation

[0029] 1.1 Culture medium configuration

[0030] Fully functional medium: Add 20% fetal bovine serum, 25ng / mL human epidermal growth factor, 2mmol / L L-glutamine, 100IU / mL penicillin, 100μg / mL Streptomycin, 1% rainbow trout serum. The above concentrations are the final concentrations of each component in the fully functional medium, and the percentages are volume percentages.

[0031] PBS buffer: take 8.00g NaCl, 0.20g KCl, 2.03g Na 2 HPO 4 12H 2 O, 0.10g KH 2 PO 4 Dissolve in triple-distilled water to a volume of 1000 mL, autoclave at 115°C for 20 minutes, and store in a -4°C refrigerator for later use.

[0032] Digestion solution: Trypsin used for cell digestion and passage was purchased from Sigma, USA. Dissolve 0.25g of trypsin in 100mL of PBS, filter and sterilize, and store in a -20°C refrigerator for later use.

[0033] D-Hank's solution: Take 8.00g NaC...

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Abstract

The invention discloses a crucian brain tissue cell line and an application thereof. The crucian brain tissue cell line is crucian brain tissue cells separated from crucian brain tissues, and the preservation number of the crucian brain tissue cell line is CCTCC No.C2015159. The crucian brain tissue cell line fills a gap in researches of the crucian brain tissue cell line, provides a new research tool for viruses capable of infecting crucian, and especially lays a foundation for deep researches of viruses adopting crucian brain tissues as a target organ.

Description

technical field [0001] The application relates to the field of crucian carp cell culture, in particular to a crucian carp brain tissue cell line and its application. Background technique [0002] Crucian carp belongs to Cyprinidae, Cyprinidae and Crucian carp in classification. Crucian carp is distributed all over my country. There are two subspecies and several varieties: subspecies Carassius auratus L and subspecies Carassius auratus gibelio Bloch; and several varieties such as red crucian carp, white crucian carp, and goldfish. In my country's freshwater fish farming industry and ornamental fish farming industry, crucian carp occupies a very important position. Therefore, it is of great significance to study crucian carp from the aspects of cell biology, molecular biology, morphology, genetics and breeding. [0003] The brain of crucian carp (Carassius auratus Linnaeus) is located below the parietal bone and consists of five parts: telencephalon, diencephalon, optic lo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071C12N7/00C12Q1/70C12R1/91
Inventor 王津津贾鹏刘莹于力何俊强郑晓聪阮周曦秦智峰史秀杰兰文升刘荭
Owner 深圳出入境检验检疫局动植物检验检疫技术中心
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