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Feed additive for preventing and treating silver carp hemorrhagic disease

A feed additive, hemorrhagic disease technology, applied in animal feed, animal feed, application, etc., to achieve the effects of significant curative effect, no toxic side effects, and simple preparation method

Inactive Publication Date: 2016-05-11
ZHEJIANG AEGIS BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0027] At present, the combination of lysozyme and Coprinus comatus polysaccharides for the prevention and treatment of silver carp haemorrhagic disease has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, the extraction of Coprinus comatus polysaccharide

[0043] Take the dry product of Coprinus comatus and add 30 times the weight of water to mix, extract at 90°C for 4 hours, take the supernatant and concentrate in vacuum, precipitate with 95% ethanol with 4 times the volume of the concentrated solution, and dissolve the precipitate in an appropriate amount of water. Deproteinize by Sevag method, precipitate with an appropriate amount of 95% ethanol, and dry in vacuum to obtain Coprinus comatus polysaccharide.

Embodiment 2

[0044] Embodiment 2, the preparation of feed additive

[0045] Take 50 parts by weight of fish meal and 5 parts by weight of lysozyme, and mix them to make feed additive A1.

[0046] Take 50 parts by weight of fish meal and 5 parts by weight of Coprinus comatus polysaccharide prepared in Example 1, and mix them to make feed additive A2.

[0047] Take 50 parts by weight of fish meal, 4 parts by weight of lysozyme, and 1 part by weight of Coprinus comatus polysaccharide prepared in Example 1, and mix them to make feed additive A3 with a weight ratio of lysozyme and Coprinus comatus polysaccharide of 4:1.

[0048] Take 50 parts by weight of fish meal, 2.5 parts by weight of lysozyme, and 2.5 parts by weight of Coprinus comatus polysaccharide prepared in Example 1, and mix them to make feed additive A4 with a weight ratio of 1:1 between lysozyme and Coprinus comatus polysaccharide.

[0049] Take 50 parts by weight of fish meal, 1.25 parts by weight of lysozyme, and 3.75 parts by ...

Embodiment 3

[0052] Embodiment 3, the application of the feed additive comprising lysozyme and Coprinus comatus polysaccharide in resisting silver carp hemorrhagic disease

[0053]In order to verify the application effect of the present invention, the feed additive of the present invention was tested in silver carp in 7 fishing grounds. Silver carp were divided into experimental group (A1-A6) and control group, each with 200 fish. Wherein, the diet of the experimental group was added with the above-mentioned feed additive according to 1% of the total weight of the feed, and the diet of the control group was fish feed of the same weight without adding the above-mentioned feed additive. The fish in each group were fed 5 times a day, and fed 60 Carry out infection experiment after 3 days, experimental group and matched group all inject the pathogenic bacteria that can cause haemorrhagic disease, observe continuously 7 days, count the dead quantity of silver carp in statistical experimental gr...

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Abstract

The invention relates to a feed additive for preventing and treating a silver carp hemorrhagic disease. The feed additive is prepared from, by weight, 50-100 parts of fish meal, 1-10 parts of lysozyme and 1-10 parts of coprinus comatus polysaccharide. Compared with a common feed additive, the feed additive for aquatic organisms is easy to prepare, reasonable in compatibility, remarkable in treatment effect and capable of remarkably lowering the fish death rate, and experimental results show that the feed additive has the special effects on the silver carp hemorrhagic disease. Due to the fact the additive contains no Western medicine ingredient, the feed additive has no toxic or side effect and is free of medicine tolerance and pollution.

Description

technical field [0001] The invention relates to the field of feed, in particular to an aquatic feed additive and its application. Background technique [0002] Fish products have high nutritional value, which is very important for improving dietary structure and improving human health. With the increasing awareness of health, consumers have higher and higher requirements for the consumption and quality of fish products. In recent years, with the rapid development of feed fish farming, silver carp has become the main fish in China because of its fast growth rate, especially in pond-type reservoirs and small reservoirs. However, with the continuous increase of breeding density and pollution of the breeding environment, the incidence of various fish diseases has also increased sharply. Due to the frequent occurrence of silver carp hemorrhagic disease and the difficulty of treatment, its survival rate has been greatly reduced, which has seriously affected professional farmers. ...

Claims

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Application Information

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IPC IPC(8): A23K50/80A23K10/22A23K20/189A23K20/163A23K20/142A23K20/174
Inventor 潘宏涛卢亚萍
Owner ZHEJIANG AEGIS BIOLOGICAL SCI & TECH CO LTD