Surface plasma resonance sensor chip and cell response detection system and method

Abstract

The invention discloses a surface plasma resonance sensor chip comprising a culture cavity, a heating device, a culture solution inflow channel and a prism. The culture cavity is provided with a culture solution inflow port and a culture solution outflow port. The heating device comprises two layers of heat conductive metal and a high-resistivity membrane interlayer structure clamped between the two layers of heat conductive metal and is of a through structure, wherein the culture cavity is embedded in the through structure. The culture solution inflow channel makes a culture solution in the culture solution inflow channel flow through one surface of the heating device before entering the culture solution inflow port of the culture cavity. The prism is located on the lower portion of the culture cavity and closely connected with the culture cavity in a sealing mode, the surface, connected with the culture cavity, of the prism is plated with a precious metal membrane. The sensor chip also comprises a transparent heat-insulating shell, and the culture cavity can be layered. Thus, the temperature of a cell growing environment can be evenly and stably maintained for a long time, and cell response detection accuracy is improved.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a surface plasmon resonance sensor chip capable of maintaining the temperature required for cell growth stably and uniformly for a long time. Background technique [0002] Cells are the basic unit of living organisms. Cell detection is helpful for in-depth understanding of the essence of life activity process, which is beneficial to disease diagnosis and drug development. Surface plasmon resonance (SPR) sensing technology has the advantages of real-time, sensitivity, and label-free. The use of SPR to detect cell responses, especially the response of cancer cells to different chemotherapy regimens, has become a research hotspot in the past decade. [0003] figure 1 is a schematic diagram of a prior art SPR sensor chip system 10 . For the convenience of description, the optical coupling device and angle adjustment device in the optical part and the A / D conversion circuit and ...

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Problems solved by technology

[0005] Since the SPR sensor chip needs to place a prism at the bottom, and the top needs to be reserved as an observation window, the temperature control measures for the upper and lower parts of the sensor chip are insufficient, and the external temperature change of the sensor chip will cause its internal temperature to fluctuate, affecting long-term detection stability

In addition, for the sensor chip based on flow culture, since the temperature of the culture solution (usually room temperature) is lower than the preset temperature (usually 37 degrees Celsius) when the culture solution is injected, the flow of the culture solution will cause a temperature gradient inside the sensor chip.

This will affect the growth of cells at different positions in the sensor chip to varying degrees, thereby affecting the consistency of the detection results

Finally, the TEC devices currently in use are mass-produced devices, and the shape is usually single, which is not conducive to the design of the temperature control structure

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