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Preparation method of sodium hyaluronate gel special for preventing intrauterine adhesion after cesarean

A technology of sodium hyaluronate and intrauterine adhesions, applied in medical science, surgery, etc., can solve the problem of difficult to prevent intrauterine adhesions, and achieve the effect of reducing the probability of repeated adhesions, reducing pain and less toxicity

Inactive Publication Date: 2016-06-01
张静
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem mainly solved by the present invention: in the process of preventing intrauterine adhesions, although the hyaluronic acid used has the effect of preventing pelvic and abdominal adhesions, it stays in the uterine cavity for a short time and is easily absorbed by hyaluronidase in the body. As well as the degradation of free radicals, it is difficult to achieve the current situation of preventing intrauterine adhesions. A kind of eyeball vitreous body is provided as a raw material. After protein purification, dialysis, concentration and drying are obtained to obtain sodium hyaluronate powder, and then tea polyphenols are extracted from tea leaves as free hyaluronate powder. Base scavenger, heparin sodium as an anti-enzymolysis additive, and the three are compounded to obtain a preparation method of sodium hyaluronate gel specially used for preventing intrauterine adhesions in caesarean section

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0021] First, weigh 1 kg of ox-eye vitreous body and put it into a 10L enamel tank, then add caustic soda solution with a concentration of 0.5mol / L into the tank according to the solid-to-liquid ratio of 1:2, stir and extract in a water bath at 60°C for 20 minutes, and then add 1L mass concentration is 10% trichloroacetic acid, adjust the pH to 7.3 with 30% potassium hydroxide solution, keep warm for 1.5 hours to remove impurity proteins, and obtain a mixed solution; then put the above mixed solution into a dialysis bag and place it in a flow Dialyze in deionized water overnight, open the dialysis bag, take out the dialyzate in the bag and filter, remove the filter residue to obtain the filtrate, then heat the obtained filtrate to 85°C for sterilizing treatment for 13 minutes, add absolute ethanol dropwise to the filtrate at a rate of 10mL / min until no more Precipitation occurs, after centrifugation to obtain the precipitate, spray drying, and grinding to obtain sodium hyaluron...

example 2

[0024] First, weigh 1.5kg of bovine eyeball vitreous body and put it into a 10L enamel tank, then add caustic soda solution with a concentration of 0.5mol / L into the tank according to the solid-to-liquid ratio of 1:2, stir and extract in a water bath at 65°C for 25 minutes, and then Add 1.5 L of trichloroacetic acid with a mass concentration of 10%, adjust the pH to 7.3 with a 30% potassium hydroxide solution, and keep warm for 1.5 hours to remove impurity proteins to obtain a mixed solution; then put the above mixed solution into a dialysis bag and place it in a Dialyze in flowing deionized water overnight, open the dialysis bag, take out the dialyzate in the bag and filter, remove the filter residue to obtain the filtrate, then heat the obtained filtrate to 85°C for sterilization for 13 minutes, add absolute ethanol dropwise to the filtrate at a rate of 10mL / min until No more precipitation occurs, after centrifugation to obtain the precipitate, spray drying, and grinding to o...

example 3

[0027] Firstly, weigh 2kg of ox-eye vitreous body and put it into a 10L enamel tank, then add caustic soda solution with a concentration of 0.5mol / L into the tank according to the solid-to-liquid ratio of 1:2, stir and extract in a water bath at 70°C for 30 minutes, and then add 2L mass concentration of 10% trichloroacetic acid, adjust the pH to 7.5 with 30% potassium hydroxide solution, keep warm for 2 hours to remove impurities, and obtain a mixed solution; then put the above mixed solution into a dialysis bag and place it in a flow deionization Dialyze in water overnight, open the dialysis bag, take out the dialyzate in the bag, filter, remove the filter residue to obtain the filtrate, then heat the obtained filtrate to 90°C for sterilization for 15 minutes, add absolute ethanol dropwise to the filtrate at a rate of 10mL / min until there is no more precipitation produced, centrifuged to obtain the precipitate, spray-dried, and ground to obtain sodium hyaluronate powder for la...

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Abstract

The invention discloses a preparation method of sodium hyaluronate gel special for preventing intrauterine adhesion after cesarean and belongs to the technical field of perborate gel preparation. According to the preparation method, eyeball vitreous bodies serve as raw materials, and after protein-removal purification is conducted, dialysis, condensation and drying are conducted, so that sodium hyaluronate powder is obtained, then tea polyphenol is extracted from tea leaves as free-radical scavenger, heparin sodium serves as an anti-enymolysis additive, the three types of constituents are compounded, and thereby the sodium hyaluronate gel specially for preventing intrauterine adhesion after cesarean is obtained. The preparation method is easy and convenient to operate and unique in method, the sodium hyaluronate gel is prepared by extracting hyaluronic acid, and the anti-enymolysis agent and the free-radical scavenger are added so that the obtained sodium hyaluronate gel is good in biological stability, is not degraded by hyaluronidase and free radicals easily and has a remarkable intrauterine adhesion prevention effect.

Description

technical field [0001] The invention discloses a preparation method of sodium hyaluronate gel specially used for preventing intrauterine adhesion during cesarean section, and belongs to the technical field of gel preparation. Background technique [0002] Intrauterine adhesions refer to the mutual adhesion of the uterine cavity, uterine isthmus, and cervical canal due to uterine cavity surgery, radiation, infection, etc. The clinical manifestations can be abdominal pain, menstrual decrease or amenorrhea, infertility and habitual abortion etc. Any factors that cause endometrial destruction can lead to intrauterine adhesions. Hysteroscopic electrosurgical resection destroys the endometrium to varying degrees, and intrauterine adhesions are prone to occur after surgery. Tissue adhesions after cesarean section mainly include the mutual adhesion of the intestinal serosa injury and the adhesion between the uterine incision and the abdominal wall. After the formation of adhesions, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/04A61L31/00A61L31/14
CPCA61L31/042A61L31/00A61L31/14A61L31/143A61L31/145
Inventor 张静张帆林大伟
Owner 张静