A method for extracting luteolin
A technology for luteolin and extract is applied in the field of separation and extraction of high-purity luteolin, and can solve the problems of complicated operation steps, long separation and extraction time, etc.
Inactive Publication Date: 2017-12-05
BEIJING UNIV OF AGRI
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Problems solved by technology
There are also related extraction methods of higher purity luteolin, such as Li Hongxia et al. (Chinese Patent Application No. 200910004229.1) disclosed "a method for separating and purifying luteolin", the steps include crude ethanol extraction, polymer preparation, extraction column purification, etc. , the operation steps are more complex and require a longer separation and extraction time
Method used
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Experimental program
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Effect test
Embodiment 1
[0016] 0.5kg decoction pieces of perilla leaves, a traditional Chinese medicine of the Lamiaceae plant, are rinsed with distilled water, the weight ratio of the decoction pieces to distilled water is 1:15, decocted twice with a YZN50 liquid vacuum concentration decoction machine, each time for 1 hour, and the combined decoction is decompressed Concentrate to crude drug concentration of 1g / ml. Add dehydrated ethanol to obtain an extract of sage leaves with an ethanol concentration of 75%, centrifuge at 4°C and 5000g for 30 minutes, discard the precipitate to obtain the ethanol supernatant of sage leaves, and recover ethanol under reduced pressure with a YZN50 liquid vacuum concentration decoction machine to obtain sage leaves The leaf concentration is 1 g / ml perilla leaf extract.
[0017] The perilla leaf extract was prepared into a 200 mg / ml perilla leaf extract solution with mobile phase, and the luteolin standard was prepared with mobile phase into 0.1 mg / ml luteolin solutio...
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A method of separating and extracting to obtain high-purity luteolin from luteolin-containing plants comprising perilla leaves, honeysuckle flower and other natural medicine materials or extract products thereof is disclosed. The method is characterized in that materials to be used are subjected to water extraction and alcohol precipitation and ethanol is recovered to obtain an extract product. The high-purity luteolin is prepared by firstly injecting a standard luteolin sample into a preparative high performance liquid chromatograph to determine the retention time of the peak of luteolin, injecting a solution of the extract product into the preparative high performance liquid chromatograph, collecting a component the retention time of which is same as the retention time of the standard luteolin sample, repeatedly injecting the solution of the extract product, collecting the component, and drying at low temperature. The method is advantageous in that through water extraction, alcohol precipitation and high performance liquid chromatography, the high-purity luteolin can be rapidly obtained by separation and extraction from the luteolin-containing plants comprising the perilla leaves, the honeysuckle flower and other natural medicine materials or the extract products thereof.
Description
technical field [0001] The invention relates to a method for separating and extracting high-purity luteolin from luteolin-containing plants such as perilla leaves, honeysuckle and other natural medicinal materials or extracts thereof. Background technique [0002] Luteolin is a natural flavonoid compound, which exists in natural medicinal materials such as perilla leaves, honeysuckle, nepeta, and vegetables such as cabbage, cauliflower, and carrots. Studies have shown that it has anti-inflammatory, anti-allergic, anti-tumor, anti-oxidation, and neuroprotective properties. System and other pharmacological effects, it is mainly used clinically for the treatment of respiratory inflammatory diseases, tumor diseases and cardiovascular diseases. [0003] At present, the extraction methods of crude luteolin include alcohol extraction, methanol extraction, etc., and further purification by polyamide chromatography. There are also related extraction methods of higher purity luteolin...
Claims
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IPC IPC(8): C07D311/30C07D311/40
Inventor 姜代勋李佳李丰男陈武
Owner BEIJING UNIV OF AGRI