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Extraction method of Rhodiola rosea entity small molecule peptide

A technology of small molecular peptides and extraction methods, which is applied in the field of extraction of Rhodiola rosea entity small molecular peptides, to achieve the effects of saving production costs, improving immunity, and enhancing memory

Inactive Publication Date: 2016-06-08
蔡庭守
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports about the extraction of Rhodiola rosea entity small molecule peptides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for extracting small-molecule peptides from Rhodiola rosea, including the following steps:

[0027] (1) Rhodiola rosea was dried at a temperature of 50℃ for 15h, and the dried Rhodiola rosea was crushed into Rhodiola rosea powder with a particle size of 80 mesh; Rhodiola rosea was crushed, which is beneficial to the rhodiola and water It is fully mixed to facilitate the precipitation of the effective ingredients in Rhodiola;

[0028] (2) Mix Rhodiola rosea and water with a weight ratio of 1:12.5 under the condition of a stirring speed of 60r / min to form a mixture, then heat it to 90℃, keep it for 10 minutes and then cool to 45℃ , Keep the temperature rising rate at 20℃ / min during heating, because when the temperature is heated to 90℃, it is beneficial to the precipitation of the effective components in Rhodiola;

[0029] (3) Add alkaline protease with an activity of 500,000 u / g to the cooled mixture, and the weight ratio of the added alkaline protease to the weight o...

Embodiment 2

[0035] A method for extracting small-molecule peptides from Rhodiola rosea, including the following steps:

[0036] (1) Rhodiola rosea was dried at a temperature of 55°C for 16 hours, and the dried Rhodiola rosea was crushed into Rhodiola rosea powder with a particle size of 100 mesh; crushing Rhodiola rosea is beneficial to the rhodiola and water It is fully mixed to facilitate the precipitation of the effective ingredients in Rhodiola;

[0037] (2) Mix Rhodiola rosea and water with a weight ratio of 1:12.5 at a stirring speed of 65r / min to form a mixture, then heat it to 95°C, keep it for 12 minutes, and then cool it to 50°C , Keep the temperature increase rate at 25℃ / min during heating, because when the temperature is heated to 95℃, it is beneficial to the precipitation of the effective components in Rhodiola;

[0038] (3) Add alkaline protease with an activity of 500,000 u / g to the cooled mixture, and the weight ratio of the added alkaline protease to the weight of rhodiola powd...

Embodiment 3

[0044] A method for extracting small-molecule peptides from Rhodiola rosea, including the following steps:

[0045] (1) Dry Rhodiola rosea at a temperature of 60℃ for 18h, and crush the dried Rhodiola rosea into Rhodiola powder with a particle size of 120 mesh; crushing Rhodiola rosea is beneficial to Rhodiola and water It is fully mixed to facilitate the precipitation of the effective ingredients in Rhodiola;

[0046] (2) Mix Rhodiola rosea and water with a weight ratio of 1:12.5 at a stirring speed of 70r / min to form a mixture, then heat it to 100°C, keep it for 15 minutes, and then cool to 55°C , Keep the temperature increase rate at 30℃ / min during heating, because when the temperature is heated to 100℃, it is beneficial to the precipitation of the effective components in Rhodiola;

[0047] (3) Add alkaline protease with an activity of 500,000 u / g to the cooled mixture, and the weight ratio of the added alkaline protease to the weight of rhodiola powder is 1:100, due to the suita...

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PUM

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Abstract

The invention discloses an extraction method of Rhodiola rosea entity small molecule peptide. The extraction method includes following steps: drying Rhodiola rosea, and smashing the same into Rhodiola rosea powder; mixing the Rhodiola rosea powder with water to form mixed liquid, heating the mixed liquid, holding temperature for a certain period of time, and cooling; adding alkaline protease into the mixed liquid after being cooled, adjusting pH, heating the mixed liquid, and holding temperature for a certain period of time to obtain an alkaline solution; adding neutral protease into the alkaline solution, adjusting pH, heating, and holding temperature for a certain period of time to obtain a neutral solution; adding pepsase into the neutral solution, adjusting pH, heating, and holding temperature to obtain an acidic solution; heating the acidic solution, holding temperature for a certain period of time, and filtering to obtain filtrate; decoloring the filtrate to obtain clear liquid, concentrating the clear liquid, and drying to obtain Rhodiola rosea entity small molecule peptide powder. The extraction method has the advantage that the small molecule peptide extracted from Rhodiola rosea entity has efficacy of improving immunity, neurological function and memory if being eaten frequently.

Description

Technical field [0001] The invention relates to an extraction method, in particular to an extraction method for Rhodiola rosea entity small molecule peptides. Background technique [0002] Small peptides are a classification of polypeptides. The molecular weight range is generally below 1000 Daltons, also called small peptides, oligopeptides or small molecule active peptides. They are generally composed of 2-6 amino acids, which do not require digestion, It is directly absorbed. It does not need to consume human energy during absorption, does not increase the burden of human gastrointestinal function, and can force the body to absorb when the body's absorption function is lost. In addition, it also has the function of carrier, which can carry other nutrients of human food in On its body, it is transported to the cells and tissues of the human body, and various trace elements are transported to various parts of the human body. [0003] However, there are very few naturally-occurrin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C07K1/14
CPCC12P21/06C07K1/14C07K1/34
Inventor 蔡庭守
Owner 蔡庭守