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An ultrasonic-assisted enzymatic method for extracting water-soluble dietary fiber from pre-acidified bean dregs

A dietary fiber and extraction method technology, which is applied in the field of ultrasonic-assisted enzymatic extraction of water-soluble dietary fiber in pre-acidified bean dregs, can solve the problems of environmental pollution, extraction effect and extraction rate can not be balanced, to reduce pollution, reduce addition and use amount, the effect of reducing the amount of acid

Active Publication Date: 2018-09-28
山东禹王生物技术有限公司
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0009] In order to solve the problem that more chemical reagents are used in the process of extracting water-soluble dietary fiber in the prior art to pollute the environment, and the extraction effect and extraction rate cannot be taken into account, the present invention provides a method of using less chemical reagents in the extraction process through research. A method for extracting water-soluble dietary fiber that is simple to operate and can take into account both the extraction rate and the extraction effect

Method used

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  • An ultrasonic-assisted enzymatic method for extracting water-soluble dietary fiber from pre-acidified bean dregs
  • An ultrasonic-assisted enzymatic method for extracting water-soluble dietary fiber from pre-acidified bean dregs
  • An ultrasonic-assisted enzymatic method for extracting water-soluble dietary fiber from pre-acidified bean dregs

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Experimental program
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Effect test

Embodiment 1

[0034] Analyze the effects of factors such as acidification pH value, water bath temperature, and ethanol precipitation addition on the SDF yield. The selected conditions and experimental results are shown below figure 1 , figure 2 , image 3 .

[0035] From figure 1 The results show that the pH value has a significant impact on the extraction rate of water-soluble dietary fiber. With the increase of pH value, the extraction rate of water-soluble dietary fiber shows a downward trend as a whole. Lower values ​​are not suitable for further testing. Therefore, when the pH value is 1.0, the extraction rate is the highest, which is more suitable.

[0036] From figure 2 The results show that temperature has a significant impact on the extraction rate of water-soluble dietary fiber. With the increase of extraction temperature, the extraction effect also shows an upward trend. ℃ to 50℃ slowly. Considering the influence of temperature on the color, water holding capacity and e...

Embodiment 2

[0039]After adding an appropriate amount of water into the bean dregs and stirring, carry out high-pressure sterilization at a steam pressure of 0.15Mpa and a temperature of 121°C for 3.5 hours. After sterilization, add hydrochloric acid solution with a concentration of 10%, adjust the pH of the feed solution to about 1.0, place it in a constant temperature water bath at 60°C and stir evenly, and filter after stirring for 10 minutes, adjust the pH of the precipitate to about 5.0, and put it in High-pressure shear homogenization in the high-pressure homogenizer, homogenization pressure 2200bar, adding 0.3‰ of enzyme activity: 0.12-0.86 million U of cellulase, the temperature is about 48°C, placed in an ultrasonic environment, the ultrasonic power is 400W , the extraction time is 50min, after the second extraction, centrifuge, add 3.5 times of 85% (w / w) ethanol to the supernatant for precipitation, centrifuge at 3500r / min, 20min, and dry the precipitate under the condition of -30...

Embodiment 3

[0041] After adding an appropriate amount of water into the bean dregs and stirring, carry out high-pressure sterilization, when the steam pressure is 0.13Mpa and the temperature is 125°C, keep it for 3.5 hours. After sterilization, add hydrochloric acid solution with a concentration of 10%, adjust the pH of the feed solution to about 1.0, place it in a constant temperature water bath at 60°C and stir evenly, and filter after stirring for 10 minutes, adjust the pH of the precipitate to about 5.0, and put it in High-pressure shear homogenization in high-pressure homogenizer, homogenization pressure 2500bar, add 0.3‰ of enzyme activity: 0.12-0.86 million U of cellulase, the temperature is about 48°C, placed in an ultrasonic environment, the ultrasonic power is 400W , the extraction time is 50min, after the second extraction, centrifuge, add 3 times of 85% (w / w) ethanol to the supernatant for precipitation, centrifuge at 3500r / min, 20min, and dry the precipitate under the conditio...

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Abstract

The invention provides a method using an ultrasonic-assisted enzyme method to extract the water-soluble dietary fibers in pre-acidified soybean dregs.The method includes: adding an appropriate amount of water into the soybean dregs, stirring, performing high-pressure sterilizing, adding a hydrochloric acid solution with the concentration being 5-15% after the sterilizing, pre-acidifying, evenly stirring in a water-bath pot, filtering, adjusting the pH of precipitate to about 5.0, placing into a high-pressure homogenizing machine for high-pressure shearing and homogenizing, adding 0.3%.of cellulase, performing ultrasonic extraction, centrifuging, adding ethanol with the weight being 3-4 times of the weight of supernatant into the supernatant for precipitation, filtering, and drying the precipitate to obtain the water-soluble dietary fibers SDF.The method has the advantages that little chemical reagent is used in the extraction process, and the method is free of pollution, simple to operate and capable of considering both the extraction efficiency and extraction effect.

Description

technical field [0001] The invention belongs to the field of food production, and in particular relates to a method for extracting water-soluble dietary fiber in pre-acidified bean dregs by an ultrasonic-assisted enzymatic method. Background technique [0002] With the improvement of people's living standards, diets are becoming more and more refined, and a large amount of high-calorie, high-protein, high-fat and refined foods are taken in, which leads to diabetes, cardiovascular disease, obesity, intestinal cancer, constipation, gallstones, fatty liver, etc. The disease of affluence is becoming more and more common. [0003] Studies have found that dietary fiber has obvious preventive and therapeutic effects on the above various diseases, has outstanding health care functions, and is recognized as the "seventh largest nutrient". [0004] Studies have shown that the solubility of dietary fiber is an important factor affecting its physiological function. The physiological fu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/24
CPCA23V2002/00A23V2250/5116
Inventor 刘汝萃王彩华牛祥臣刘军王洪彩
Owner 山东禹王生物技术有限公司