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Nano-micron multiscale tissue engineering composite three-dimension bracket and preparation method thereof

A technology of three-dimensional scaffolding and tissue engineering, applied in non-woven fabrics, textiles, papermaking, medical science, etc., can solve the problems of uncontrollable fiber diameter and pore size, achieve good biocompatibility and mechanical strength, simple process, and pore size easy effect

Active Publication Date: 2016-06-15
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, researchers mostly use the method of phase separation to prepare three-dimensional scaffolds, but they cannot control the fiber diameter and pore size, or use electrospinning to obtain two-dimensional fiber films, but how to prepare fiber diameters and pore sizes with micro-nano multi-scale The preparation method of three-dimensional chitosan sodium alginate composite scaffold has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Take 7ml of trifluoroacetic acid and 3ml of dioxane respectively, mix them evenly, weigh 0.5g of chitosan, stir to dissolve, and let stand for 12 hours. Take 10ml of water, weigh 0.3g of sodium alginate, stir to dissolve, and let stand for 12 hours. Add chitosan and sodium alginate solutions into two syringes respectively, connect the two syringes with a two-in-one needle, spin with a voltage of 20kV, and collect them on a grounded aluminum foil to obtain chitosan alginate Sodium nanocomposite fiber film, the fiber diameter is between 50-500nm. Take 9ml of trifluoroacetic acid and 1ml of dioxane respectively, mix them evenly, weigh 0.75g of chitosan, stir to dissolve, and let stand for 12 hours. Take 10ml of water, weigh 0.3g of sodium alginate, and let it stand for 12 hours. Add chitosan and sodium alginate solutions to two syringes respectively, connect the two syringes with a two-in-one needle, spin with a voltage of 15kV, and collect them on a grounded ...

Embodiment 2

[0016] Example 2: Take 14ml of trifluoroacetic acid and 6ml of dioxane respectively, mix them evenly, weigh 1.0g of chitosan, stir to dissolve, and let stand for 12 hours. Take 20ml of water, weigh 0.6g of sodium alginate, stir to dissolve, and let stand for 12 hours. Add chitosan and sodium alginate solutions into two syringes respectively, connect the two syringes with a two-in-one needle, spin with a voltage of 25kV, and collect them on a grounded aluminum foil to obtain chitosan alginate Sodium nanocomposite fiber film, the fiber diameter is between 50-500nm. Take 9ml of trifluoroacetic acid and 1ml of dioxane respectively, mix them evenly, weigh 0.75g of chitosan, stir to dissolve, and let stand for 12 hours. Take 10ml of water, weigh 0.6g of sodium alginate, stir to dissolve, and let stand for 12 hours. Add chitosan and sodium alginate solutions to two syringes respectively, connect the two syringes with a two-in-one needle, spin with a voltage of 12kV, and collect the...

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Abstract

The invention relates to a nano-micron multiscale tissue engineering composite three-dimension bracket and a preparation method thereof. Electrostatic spinning is performed under certain conditions to obtain a nano-micron chitosan sodium alginate composite fiber membrane; after the composite fiber membrane is subjected to alkaline washing and drying, the nano-micron chitosan sodium alginate composite fiber membrane is sheared into short fiber at high speed in water according to a certain proportion; after sample separation, calcium nitrate particles in micron scale are added as a pore-foaming agent; after freezing, drying and formation, water displacement is performed to remove the pore-foaming agent; then, freezing and drying are preformed to obtain the nano-micron multiscale chitosan sodium alginate composite three-dimension bracket. Chitosan and sodium alginate are compounded to overcome the defects of the chitosan and the sodium alginate. The preparation method has the advantages that the process is simple; the fiber diameter and the hole diameter of the bracket simultaneously have the nano-micron scale, so that good biology performance and mechanical performance are given to the composite brace.

Description

technical field [0001] The invention relates to a nano-micron multi-scale tissue engineering composite three-dimensional scaffold and a preparation method thereof, belonging to the technical field of biomedical materials. Background technique [0002] As a cationic alkaline polysaccharide, chitosan is similar in structure to polysaccharides such as chondroitin sulfate and hyaluronic acid in the human body. Excellent properties such as reducing scars have important applications in the field of biomedical materials. SundararajanV et al. used phase separation technology to prepare chitosan scaffolds for the first time. Its structure is a sheet-like honeycomb structure with a thickness of tens to hundreds of microns, which has good biological properties (Biomaterials, 1999; 20:1133-1142), However, scaffolds made of chitosan are easy to swell and have poor structural stability. Sodium alginate can be extracted from natural brown algae. It is an anionic polysaccharide composed o...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/56D04H1/728
CPCA61L27/20A61L27/56D04H1/728C08L5/08C08L5/04
Inventor 楼涛汪学军
Owner QINGDAO UNIV