Method for Determination of Verapamil Hydrochloride Content in Medicine Using Electrochemiluminescence Behavior of Composite Modifier-loaded Pyrolytic Graphite Electrode
A technology of verapamil hydrochloride and pyrolytic graphite, which is applied in electrical excitation analysis, material excitation analysis, etc., can solve the problems of polluted environment and operation, low utilization efficiency and high test cost, and achieves easy cracking and high sensitivity. , the effect of a wide linear range
Inactive Publication Date: 2018-04-06
GUANGXI UNIVERSITY OF TECHNOLOGY
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Problems solved by technology
In summary, when using the drop method to measure verapamil hydrochloride, there is Ru(bpy) 3 2+ Large amount of use, low utilization efficiency, high test cost, environmental pollution and inconvenient operation, etc., and the measurement effect needs to be further improved
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Embodiment 1
[0040] Embodiment 1: a kind of method utilizing composite modifier loaded pyrolytic graphite electrode electrochemiluminescence behavior to measure verapamil hydrochloride content in medicine, comprises the following steps:
[0041] (1) Preparation of nano-titanium dioxide sol
[0042] 4 mL TiCl 4 Pass through 100 mL ethanol solution, take 20 mL TiCl 4 -EtOH solution and adjust pH=8.8 with 37 mL 2.5% ammonia water, filter with suction, wash the filter cake with deionized water until neutral, wash with 30 mL H 2 o 2 dissolved, then added 5 mL of silica sol and 65 mL of water, and refluxed at 95 °C for 4 h.
[0043] (2) Preparation of Nano-Ag
[0044] Using silver nitrate as the precursor and PVP as the stabilizer and reducing agent, 0.169 g of silver nitrate was dissolved in 50 mL ethanol solution with rapid stirring, and dispersed at 40 °C for 10 min; at the same time, 1 g of PVP was added to 50 mL Dissolve in ethanol at 60°C for 10 min, then add silver nitrate solution d...
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The invention relates to a method for determination of the content of verapamil hydrochloride in a drug by using an electrogenerated chemiluminescence behavior of a composite modifier-supported pyrolytic graphite electrode; with use of an auxiliary film forming property of TiO2 sol and silica sol and a good electrocatalytic property of ZnO@Ag, a TiO2 sol / ZnO@Ag / silica sol composite modifier is prepared; the surface of a pyrolytic graphite electrode is supported with bipyridine ruthenium by a sensor modification technology, an electrogenerated chemiluminescence sensor which is used for determination of verapamil hydrochloride and has good stability and high sensitivity is prepared, and simple and rapid determination of verapamil hydrochloride is achieved. Under optimal test conditions, 1.0*10<-7>-1.0*10<-4> mol / L verapamil hydrochloride has a linear relationship (R<2>=0.9731) with the relative luminescence intensity, and a detection limit (S / N=3) is 7.11*10<-9> mol / L. The method has relatively high selectivity and sensitivity for determination of verapamil hydrochloride, and realizes repeated use of bipyridine ruthenium.
Description
technical field [0001] The invention relates to a method for measuring the content of verapamil hydrochloride in medicine, in particular to a method for measuring the content of verapamil hydrochloride in medicine by using the electrochemiluminescence behavior of a pyrolytic graphite electrode loaded with a composite modifier. Background technique [0002] Verapamil hydrochloride (Verapamil hydrochloride) is a calcium ion antagonist, which can selectively expand coronary arteries and increase coronary flow; it can inhibit myocardial excitability and atrioventricular conduction, and is commonly used in clinical antiarrhythmia, antiangina pectoris and treatment Drugs for hypertension. At present, in the method for determining verapamil hydrochloride, mainly based on high performance liquid chromatography, also includes reversed phase high performance liquid phase-fluorescence detection chromatography, capillary electrophoresis, flow injection chemiluminescence, electrochemical...
Claims
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IPC IPC(8): G01N21/66
CPCG01N21/66
Inventor 李彦青罗应李利军程昊黄文艺冯军孔红星魏慧丹史智鹏
Owner GUANGXI UNIVERSITY OF TECHNOLOGY