Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for constructing hplc fingerprint of Chinese patent medicine "Erding Granules"

A construction method and technology of fingerprints, applied in the field of construction of HPLC fingerprints of Chinese patent medicines, can solve problems such as difficult to reflect products as a whole, control product quality, etc., to avoid singleness and one-sidedness, good reproducibility and precision high effect

Active Publication Date: 2017-12-01
XIUZHENG PHARMA GRP CHANGCHUN HIGH NEW PHARMA CO LTD +1
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its quality standards only control the content determination of one component of the blind medicine and the qualitative identification of a single component, and it is difficult to reflect the quality of the product as a whole or to control the quality of the product as a whole
[0003] Traditional Chinese medicine and its preparations are multi-component complex systems, so the evaluation of its quality should adopt detection methods that are suitable for it and can provide rich identification information, but the current methods of microscopic identification, physical and chemical identification and content determination are not enough solve this problem

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for constructing hplc fingerprint of Chinese patent medicine "Erding Granules"
  • A method for constructing hplc fingerprint of Chinese patent medicine "Erding Granules"
  • A method for constructing hplc fingerprint of Chinese patent medicine "Erding Granules"

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: Erding particle quality control method based on fingerprint

[0043] Instrument: L3000‐Rigol high performance liquid chromatograph, MS205DU analytical balance

[0044] Reagents: terpetin B reference substance, methanol for liquid chromatography analysis is chromatographically pure, other reagents are analytically pure, water is ultrapure water, and Erding granules are provided by Changchun High-tech Pharmaceutical Co., Ltd. of Xiuzheng Pharmaceutical Group.

[0045] Preparation of the test solution: Get about 2 g of diding granule fine powder, accurately weigh it, put it in a stoppered conical flask, add 25 ml of 70% methanol accurately, weigh it, extract it by ultrasonic for 20 minutes, weigh it again, and use Make up the lost weight with 70% methanol, shake well, filter, take the subsequent filtrate, and filter it with a 0.45 μm microporous membrane to obtain the product.

[0046] Preparation of the reference substance solution: take an appropriate amou...

Embodiment 2

[0051] Embodiment 2: The quality control method of Erding particle based on fingerprint

[0052]Instrument: L3000‐Rigol high performance liquid chromatograph, MS205DU analytical balance

[0053] Reagents: acetaminophen B reference substance, reagents for liquid chromatography analysis are chromatographically pure, other reagents are analytically pure, water is ultrapure water, and Erding granules are provided by Changchun High-tech Pharmaceutical Co., Ltd. of Xiuzheng Pharmaceutical Group.

[0054] Preparation of the test solution: Get about 5 g of diding granule fine powder, accurately weigh it, place it in a stoppered conical flask, add 50 ml of 50% methanol accurately, weigh it, extract it by ultrasonic for 40 minutes, weigh it again, and use Make up the lost weight with 50% methanol, shake well, filter, take the subsequent filtrate, and filter it with a 0.45 μm microporous membrane to obtain the product.

[0055] Preparation of the reference substance solution: Take an ap...

Embodiment 3

[0060] Embodiment 3: Erding particle quality control method based on fingerprint

[0061] Instrument: Agilent 1200 high performance liquid chromatograph, MS205DU analytical balance

[0062] Reagents: acetaminophen B reference substance, reagents for liquid chromatography analysis are chromatographically pure, other reagents are analytically pure, water is ultrapure water, and Erding granules are provided by Changchun High-tech Pharmaceutical Co., Ltd. of Xiuzheng Pharmaceutical Group.

[0063] Preparation of the test solution: Get about 5 g of diding granule fine powder, accurately weigh it, put it in a stoppered conical flask, add 25 ml of 70% methanol accurately, weigh it, extract it by ultrasonic for 30 minutes, weigh it again, and use Make up the lost weight with 70% methanol, shake well, filter, take the subsequent filtrate, and filter it with a 0.45 μm microporous membrane to obtain the product.

[0064] Preparation of the reference substance solution: take an appropria...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a HPLC fingerprint chromatogram establishing method of Chinese patent medicine 'dibutyl particles' and belongs to the technical field of traditional Chinese medicines.The method includes the steps that 1, a reference substance solution is prepared; 2, a test solution is prepared; 3, the chromatographic conditions are that: an Agilent ZORBAX SB-C18 (5 microns, 4.6*250 mm) column is a chromatographic column, a mobile phase A is methanol, a mobile phase B is a phosphoric acid water solution, the flow rate for gradient elution is 1 ml / min, the column temperature is 30-40 DEG C, and the detection wavelength is 254-380 nm; 4, fingerprint chromatogram determination is performed by adopting high performance liquid chromatography.By comparing the HPLC fingerprint chromatograms of 10 batches of dibutyl particles, eight common peaks are determined, and the standard fingerprint chromatogram of the dibutyl particles is established.The method has the advantages of facilitating the comprehensive monitoring of the product quality and being convenient, quick, high in precision, good in reproducibility and the like, and can accurately and reliably control the quality of the dibutyl particles.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a method for constructing HPLC fingerprints of Chinese patent medicines. Background technique [0002] The 2015 edition of the Chinese Pharmacopoeia discloses the quality control method of the heat-clearing and detoxifying Chinese patent medicine (trade name: Erding Granules) composed of four flavors of Zihua Diding, lobelia, dandelion, and Radix Radix, including the properties of the granules; TLC identification of B component and caffeic acid component in dandelion; content determination of teretin B. Its quality standards only control the content determination of one component of the blind medicine and the qualitative identification of a single component, which makes it difficult to reflect the quality of the product as a whole or to control the quality of the product as a whole. [0003] Traditional Chinese medicine and its preparations are ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 徐云任晶赵源慧白冰徐建赵俞
Owner XIUZHENG PHARMA GRP CHANGCHUN HIGH NEW PHARMA CO LTD