A kind of cultivation method of high-yield and high-quality selenium-enriched Cordyceps militaris
A technology of selenium-enriched Cordyceps militaris and its cultivation method, which is applied in the field of high-yield and high-quality selenium-enriched Cordyceps militaris cultivation, can solve problems such as difficulty in guaranteeing product quality, excessive aerial hyphae, and affecting the production of selenium-enriched Cordyceps militaris. quality, promoting mycelial growth, increasing yield and the effect of selenium enrichment rate
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Embodiment 1
[0018] The specific technological process of bottle planting of the present invention is as follows:
[0019] Step 1: Peel the potatoes and cut them into pieces, weigh 200g / L of potatoes, boil them in an appropriate amount of tap water for 20 minutes, filter them with three layers of gauze, add glucose, peptone, yeast powder, potassium dihydrogen phosphate, magnesium sulfate, Triamine Citrate, Vitamin B 1 , the formula is potato 200g / L, glucose 20g / L, peptone 15g / L, potassium dihydrogen phosphate 2g / L, magnesium sulfate 1g / L, triammonium citrate 1g / L, vitamin B 1 50mg / L, heated until melted to make basic culture medium;
[0020] Step 2: adding sodium selenite to the basic cultivation culture solution of step 1, the final concentration of sodium selenite is 0.16g / L, making selenium-enriched cultivation culture solution;
[0021] Step 3: Select 500mL culture bottles, add 30g of wheat into each bottle, 50mL of selenium-enriched cultivation culture solution, that is, the ratio ...
Embodiment 2
[0028] The potted plant concrete technological process of the present invention is as follows:
[0029] Step 1: Peel the potatoes and cut them into pieces, weigh 200g / L of potatoes, boil them in an appropriate amount of tap water for 20 minutes, filter them with three layers of gauze, add glucose, peptone, yeast powder, potassium dihydrogen phosphate, magnesium sulfate, lemon in turn Acid Triamine, Vitamin B 1 , the formula is potato 200g / L, glucose 20g / L, peptone 15g / L, potassium dihydrogen phosphate 2g / L, magnesium sulfate 1g / L, triammonium citrate 1g / L, vitamin B 1 50mg / L, heated until dissolved, and made into basal culture medium;
[0030] Step 2: adding sodium selenite to the basic cultivation culture solution of step 1, the final concentration of sodium selenite is 0.16g / L, making selenium-enriched cultivation culture solution;
[0031]Step 3: Select culture pots, add 500g of wheat into each pot, 850mL of selenium-enriched culture medium, soak at room temperature for ...
Embodiment 3
[0038] The present invention cultivates high-yield and high-quality selenium-enriched Cordyceps militaris process and positive effect test results are as follows:
[0039] 1. Screening of medium soaking time before sterilization
[0040] Table 1 Effects of different soaking times on biological efficiency and selenium enrichment rate
[0041]
[0042] Note: CK means no selenium-enriched and no soaking treatment group; the same letter in the same column means no significant difference between groups at P<0.05 level.
[0043] It can be seen from Table 1 that soaking the medium before sterilization can significantly improve the selenium enrichment and selenium tolerance of Cordyceps militaris. Under the condition of selenium addition of 100mg / kg, when the soaking time is 0h, its biological efficiency is only 76.23%, slightly lower than the control group (without selenium enrichment), indicating that under this condition, inorganic selenium had a certain inhibitory effect on th...
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