LC-Q-TOF/MS detection technology for 544 pesticide residues in leaf vegetables
A Q-TOF, pesticide residue technology, applied in measuring devices, material analysis by electromagnetic means, instruments, etc., can solve problems such as high price, loopholes in food safety monitoring, and only 2 or 3 years of validity, achieving a large amount of information. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0065] An implementation example of LC-Q-TOF / MS detection and confirmation technology for 544 pesticides (see Table 1) in leafy vegetables (taking celery as an example) includes the following steps:
[0066] 1. Specific steps of sample pretreatment technology:
[0067] 1.1 Take the edible part of the celery sample, chop it up, mix it evenly, seal it, and mark it;
[0068] 1.2 Weigh 10g of celery sample (accurate to 0.01g), add 40mL of 1% acetic acid acetonitrile into an 80mL centrifuge tube, use a high-speed homogenizer at 13500r / min, extract the homogenate for 1min, add 1g of sodium chloride, 4g of anhydrous magnesium sulfate , shake for 5 min, centrifuge at 4200 r / min for 5 min, take 20 mL of the supernatant, concentrate it to about 1 mL by rotary evaporation in a 40°C water bath, and wait for purification.
[0069] 1.3 in Carbon / NH 2 Add about 2cm high anhydrous sodium sulfate to the column. Rinse the SPE column with 4 mL of acetonitrile + toluene (3+1, v / v) first, and d...
Embodiment 2
[0099] Example of LC-Q-TOF / MS detection and confirmation technology for 544 pesticides (see Table 1) in leafy vegetables (taking mustard greens as an example).
[0100] The sample pretreatment steps and LC-Q-TOF / MS operating conditions refer to the processing conditions in Example 1.
[0101] Measure the sample solution in full-scan mode, compare the detection results with the TOF database, and obtain the first-level TOF score, see Figure 4 ; For compounds whose scores meet the requirements, establish a secondary acquisition method in the software, see Figure 5 ;In MS / MS mode, re-run the sample solution to obtain the full scan data of the sample fragment ions, compare it with the fragment ion information in the MS / MS library, and obtain the secondary QTOF score, see Image 6 .
[0102] LC-Q-TOF / MS detection results in leafy mustard samples in a provincial capital city: 12 commercial leafy mustard samples were collected in a provincial capital city, and 544 pesticide residu...
Embodiment 3
[0107] Implementation example of LC-Q-TOF / MS detection and confirmation technology for 544 pesticides (see Table 1) in leafy vegetables (taking lettuce as an example).
[0108] The sample pretreatment steps and LC-Q-TOF / MS operating conditions refer to the processing conditions in Example 1.
[0109] Measure the sample solution in full-scan mode, compare the detection results with the TOF database, and obtain the first-level TOF score, see Figure 7 ; For compounds whose scores meet the requirements, establish a secondary acquisition method in the software, see Figure 8 ;In MS / MS mode, re-run the sample solution to obtain the full scan data of the sample fragment ions, compare it with the fragment ion information in the MS / MS library, and obtain the secondary QTOF score, see Figure 9 .
[0110] LC-Q-TOF / MS detection results in lettuce samples in a provincial capital city: 9 samples of lettuce sold in a provincial capital city were collected, and 544 kinds of pesticide resi...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More