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Large-scale preparation method of bursin and application of bursin to prevention and control of serious livestock and poultry diseases

A technology of tripeptide capsulin and its application, which is applied in the fields of immunology, veterinary medicine and veterinary pharmacy, can solve the problems of reducing cost, complicated steps, low activity of tripeptide capsin, etc., and achieves the effect of improving immune effect.

Inactive Publication Date: 2016-07-13
ANJING BIO TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although some methods for synthesizing tripeptide bursin have been disclosed in the prior art, the steps are cumbersome and the cost cannot be greatly reduced, or the activity of the synthesized tripeptide bursin is low

Method used

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  • Large-scale preparation method of bursin and application of bursin to prevention and control of serious livestock and poultry diseases
  • Large-scale preparation method of bursin and application of bursin to prevention and control of serious livestock and poultry diseases
  • Large-scale preparation method of bursin and application of bursin to prevention and control of serious livestock and poultry diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] Embodiment 1: Enzymatic synthesis of tripeptide vesicin

[0101] 1. Preparation of BOC-His(BOC)-OSU:

[0102] Weigh 106.6 g of BOC-His(BOC)-OH (300 mg) and dissolve it in 500 ml of THF, add 34.5 g of HOSU (300 mg) to completely dissolve, cool the salt to below -5°C in an ice bath, and add dropwise 61.8 gram of DCCI in THF, and the reaction solution was placed in the refrigerator overnight after the addition. The next day, the DCU solid was filtered off, the filtrate was concentrated to dryness under reduced pressure, 150 ml of hot isopropanol was added to dissolve the residue, and after cooling, 500 ml of n-hexane was added to precipitate a solid, which was collected by filtration to obtain 113.4 g, with a yield of 83.6 %, melting point: 66.2-68°C.

[0103] 2. BOC-His(BOC)-Gly-NH 2 preparation:

[0104] Weigh 112.2 grams of BOC-His(BOC)-OSU (248 mg molecules) and dissolve in 300 milliliters of THF, add 27.2 grams of HCl-Gly-NH 2 (248 mg molecules), after mixing, slow...

Embodiment 2

[0121] Embodiment 2: Bursulin tripeptide is used for the immunoenhancing effect of swine fever and porcine blue-ear disease vaccine

[0122] 1. Experimental steps

[0123] Oral administration

[0124] 1. Animal grouping

[0125] Simultaneously, 40 weaned piglets (same species, same feeding conditions, similar body weight) were randomly divided into control group, low dose group (5ug / kg), medium dose group 15ug / kg, high dose group 30ug / kg, control group did not add bursin Tripeptide, the low-dose group, the middle-dose group, and the high-dose group were added bursin tripeptide in the feed or water at a dose of 1ug / kg, 10ug / kg and 30ug / kg every day, and fed continuously for 7 days.

[0126] Vaccine immunization was carried out according to routine immunization procedures.

[0127] 2. Measuring indicators

[0128] (1) The law of the rise and fall of swine fever and PRRS antibodies

[0129] Blood was collected in the first week, third week and sixth week, serum was separated...

Embodiment 3

[0157] Formulation of vaccine compositions

[0158] Apply tripeptide bursin to porcine vaccine adjuvant to prevent and treat swine fever or porcine blue ear disease. In Example 2, the effects of different doses of tripeptide bursin on the immune function and mechanism were studied.

[0159] In the commercially available swine fever vaccine, add 100, 200, 500, or 1000 micrograms of tripeptide bursin per 1000 milliliters (or 1000 g) of animal vaccine to prepare 4 specifications of tripeptide bursin containing swine fever vaccine .

[0160] When in use, it is administered to pigs at a dose of 5 micrograms of tripepteptipystin / kg. The results showed that after inoculation, tripeptipystin could significantly increase the serum levels of IL-6 and IL-2 in vaccinated pigs, suggesting that it could obviously promote the immune function of pigs.

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Abstract

The invention relates to a large-scale preparation method of bursin and an application of bursin to prevention and control of serious livestock and poultry diseases. The invention further relates to a medical composition containing bursin, and a preparation method and application of the medical composition. It is shown by experiment that bursin can effectively improve immunological competence of classical swine fever vaccine, porcine reproductive and respiratory syndrome vaccine, and poultry vaccine, and has direct and obvious social economic benefit.

Description

technical field [0001] The invention relates to the fields of veterinary medicine, veterinary medicine and immunology. Specifically, the present invention relates to the large-scale preparation of tripeptide bursin and its application as a vaccine adjuvant in the prevention and control of major diseases of livestock and poultry. Background technique [0002] Bursin tripeptide (also known as "tripeptide bursin") is a tripeptide called Bursin that Audhya first isolated from the bursa of Fabricius in 1986. The amino acid sequence is Lys-His-Gly-NH 2 , the function is to induce the differentiation of B cell precursors in birds and mammals into B cells, a selective differentiation hormone, and plays an important role in regulating humoral immunity (T. Audhye et al, Science, 231, 997, 1986). [0003] In view of the low immune function of livestock and poultry populations and the low level of vaccine immunity commonly faced in the current large-scale and intensive breeding industr...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61P37/04A61P31/14C07K5/09
CPCY02A40/70
Inventor 邵钧费俭王丙云石嘉豪徐来根
Owner ANJING BIO TECH SHANGHAI
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