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A method for preparing brushite bone cement porous scaffold by freeze-drying method

A technology for preparing brushite and freeze-drying method, which is applied in the field of porous brushite bone cement scaffold and its preparation, can solve the problems of complicated steps and increased cost, and achieve good porous performance, no cross-linking agent addition, Effects suitable for mass production

Active Publication Date: 2018-11-16
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the traditional freeze-drying method has complicated steps, and usually requires multiple steps such as pre-freezing, adding a cross-linking agent, and freeze-drying, which increases the cost of material preparation.

Method used

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  • A method for preparing brushite bone cement porous scaffold by freeze-drying method
  • A method for preparing brushite bone cement porous scaffold by freeze-drying method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Weigh 3g (30wt%) of calcium dihydrogen phosphate monohydrate, 7g (70wt%) of β-tricalcium phosphate, and use absolute ethanol as the medium to mill at 400rpm for 4 hours, remove the ethanol by rotary evaporation at 60°C, and dry to obtain bone cement powder for use . Weigh 4.2g (1M) of citric acid, 0.02g (1%) of phosphorylated chitosan, and 0.02g (1%) of hydroxypropyl methylcellulose, dissolve them in 20mL of ultrapure water, and stir them mechanically to dissolve them. Obtain bone cement solidification solution. Mix the powder and solidification liquid evenly according to the solid / liquid ratio of 0.7g / mL, pour it into a tetrafluoroethylene mold, close one end and open the other end, let it stand for 2 minutes to pre-solidify, then put it in a -80°C refrigerator to freeze completely , Freeze-dried for more than 48h. It is measured that the pores of the bone cement scaffold are interconnected with each other, the pore size is 50-200 μm, and the porosity is 45%.

Embodiment 2

[0027] Weigh 3g (30wt%) of calcium dihydrogen phosphate monohydrate, 7g (70wt%) of β-tricalcium phosphate, and use absolute ethanol as the medium to mill at 400rpm for 4 hours, remove the ethanol by rotary evaporation at 60°C, and dry to obtain bone cement powder for use . Weigh 4.2g (1M) of citric acid, 0.02g (1%) of phosphorylated chitosan, and 0.02g (1%) of hydroxypropyl methylcellulose, dissolve them in 20mL of ultrapure water, and stir them mechanically to dissolve them. Obtain bone cement solidification solution. Mix the powder and solidification liquid evenly according to the solid / liquid ratio of 0.6g / mL, pour it into a tetrafluoroethylene mold, close one end and open the other end, let it stand for 2 minutes to pre-solidify, then put it in a -80°C refrigerator to freeze completely , Freeze-dried for more than 48h. It is measured that the pores of the bone cement scaffold are interconnected with each other, the pore size is 50-200 μm, and the porosity is 50%.

Embodiment 3

[0029] Weigh 3g (30wt%) of calcium dihydrogen phosphate monohydrate, 7g (70wt%) of β-tricalcium phosphate, and use absolute ethanol as the medium to mill at 400rpm for 4 hours, remove the ethanol by rotary evaporation at 60°C, and dry to obtain bone cement powder for use . Weigh 4.2g (1M) of citric acid, 0.02g (1%) of phosphorylated chitosan, and 0.02g (1%) of hydroxypropyl methylcellulose, dissolve them in 20mL of ultrapure water, and stir them mechanically to dissolve them. Obtain bone cement solidification solution. Mix the powder and solidification liquid evenly at a solid / liquid ratio of 0.5g / mL, pour it into a tetrafluoroethylene mold, close one end and open the other end, let it stand for 2 minutes to pre-solidify, then put it in a -80°C refrigerator to freeze completely , Freeze-dried for more than 48h. It is measured that the pores of the bone cement scaffold are interconnected with each other, the pore size is 100-300 μm, and the porosity is 63%.

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Abstract

A method for preparing a brushite bone cement porous support by a freeze drying process comprises: by making use of the characteristic of brushite bone cement that it sets fast, mixing powder and a setting liquid in a suitable solid / liquid ratio range (0.3 g / mL-0.7 g / mL), adding a mixture into a grinder, presetting in a short time (2 min) and then carrying out freeze drying; the steps of a traditional freeze drying process to prepare bone cement supports are greatly simplified; the prepared bone cement porous support is higher than 45% in porosity, pores are communicated with one another, and pore size is 50-300 Mum.The preparation of the brushite bone cement porous support provided herein is simple and feasible, is suitable for large-scale application and has a promising application prospect in the field of clinical bone tissue repairing.

Description

technical field [0001] The invention relates to a method in the technical field of biomedical materials, in particular to a brushite bone cement porous scaffold prepared by a freeze-drying method and a preparation method thereof. Background technique [0002] As a bone repair material, the porosity of calcium phosphate cement can increase the surface area of ​​the material, on the one hand, it can provide a larger area for cell adhesion, facilitate cell adhesion, and allow vascular tissue ingrowth [Yao Jun, et al, Biomaterials, 2005], on the other hand, it is conducive to full contact between materials and body fluids, and accelerates the degradation of materials. Commonly used calcium phosphate bone cement pore-inducing methods include adding carbonate porogen gas pore formation, adding gelatin microspheres and paraffin microsphere porogens, and preparing bone cement scaffolds by freeze-drying. Among them, the traditional freeze-drying method has complicated steps, and usu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/12A61L27/20A61L27/56
Inventor 何丹农杨迪诚严一楠庄园园祝闪闪刘训伟
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH