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Small-molecule peptides of sunflower head as well as preparation method and medical application of small-molecule peptide

A technology of small molecular peptides and sunflower discs, which is applied in the field of preparation of small molecule peptides for the prevention and/or treatment of gout, and can solve the problems of many additions and subtractions of decoctions, difficulty in unifying syndrome differentiation, and less fixed dosage forms, etc. , achieve the effect of preventing and/or treating gout, simple extraction and separation process, and small protein molecular weight

Active Publication Date: 2016-07-13
洮南市金塔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still the following problems in the traditional Chinese medicine treatment of gout: there is still a lack of consensus on the pathogenesis of gout, and it is difficult to unify syndrome differentiation and typing; there are many clinical reports, which affect the reliability of the treatment results due to lack of comparison; There are many additions and subtractions, few fixed dosage forms, and the mechanism of drug action is lacking in animal experiments.
However, this patent document only mentions that the sunflower disc hydrolyzed composite powder is obtained by enzymatically hydrolyzing the sunflower disc soaking solution to obtain small molecule polypeptides, without disclosing more technical information

Method used

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  • Small-molecule peptides of sunflower head as well as preparation method and medical application of small-molecule peptide
  • Small-molecule peptides of sunflower head as well as preparation method and medical application of small-molecule peptide
  • Small-molecule peptides of sunflower head as well as preparation method and medical application of small-molecule peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Preparation of sunflower disc small molecule peptides:

[0035] 1) Crush 5 kg of sunflower discs to 100 mesh, add 30 kg of deionized water, stir well, adjust the pH to 8 with sodium hydroxide, and stir at 45°C for 120 minutes to obtain a sunflower disc protein separation solution;

[0036] 2) Heat the sunflower disk protein separation solution to 85°C, stir for 1 hour, cool to 45°C, add compound protease (ginger protease, snail protease, bat moth larvae protein digestion enzyme) accounting for 0.1% of the total volume of the sunflower disk protein separation solution : 0.5:1 mass ratio), enzymatic hydrolysis for 3 hours, when the reaction is terminated, the temperature was raised to 85°C within 5 minutes, and kept for 5 minutes to inactivate the complex protease, and the obtained enzymatic hydrolyzate was used for later use;

[0037] 3) Centrifuge the enzymolysis solution in step 2) at 5,000 rpm for 8 minutes, collect the supernatant, and obtain the crude sunflower prot...

Embodiment 2

[0041] Preparation of sunflower disc small molecule peptides:

[0042] 1) Crush 7 kg of sunflower discs to 150 mesh, add 56 kg of deionized water, stir well, adjust the pH to 8.5 with sodium hydroxide, and stir at 48°C for 180 minutes to obtain a sunflower disc protein separation solution;

[0043] 2) Heat the sunflower disk protein separation solution to 88°C, stir for 2 hours, cool to 48°C, add 0.2% of the total volume of the sunflower disk protein separation solution (ginger protease, snail protease, bat moth larvae protein digestion enzyme by 0.9 : 0.6:1 ratio), enzymatic hydrolysis for 4 hours, when the reaction is terminated, the temperature was raised to 88°C within 5 minutes, and kept for 8 minutes to inactivate the complex protease, and the obtained enzymatic hydrolyzate was used for later use;

[0044] 3) Centrifuge the enzymatic hydrolysis solution in step 2) at 5000 rpm for 10 minutes, collect the supernatant, and obtain the crude sunflower protein small molecule p...

Embodiment 3

[0048] Preparation of sunflower disc small molecule peptides:

[0049] 1) Crush 8 kg of sunflower discs to 200 mesh, add 80 kg of deionized water, stir well, adjust the pH to 9 with sodium hydroxide, and stir at 50°C for 240 minutes to obtain a sunflower disc protein separation solution;

[0050] 2) Heat the sunflower dish protein separation solution to 90°C, stir for 3 hours, cool to 50°C, add compound protease (ginger protease, snail protease, bat moth larva protein digestion enzyme according to 1.2% of the total volume of the sunflower dish separation protein solution) : 0.4:0.8 mass ratio), enzymatic hydrolysis for 5 hours, when the reaction is terminated, the temperature was raised to 90°C within 5 minutes, and kept for 10 minutes to inactivate the compound protease, and the obtained enzymatic hydrolyzate was used for later use;

[0051] 3) Centrifuge the enzymolysis solution in step 2) at 5000 rpm for 12 minutes, collect the supernatant, and obtain the crude sunflower pr...

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Abstract

The invention discloses novel plant small-molecule peptides, particularly relates to small-molecule peptides of a sunflower head as well as a preparation method and medical application of the small-molecule peptides, and further discloses medical application of the small-molecule peptides of the sunflower head to treatment of gout diseases. The small-molecule peptides of the sunflower head are low in molecular weight of proteins and can be directly absorbed by a human body without the digestion system; the peptides can be absorbed quickly and takes effect efficiently; a related extracting and separating process is simple and is easy to operate; no special equipment is required, and the cost is low; reagents used are pollution-free to the environment. The small-molecule peptides are suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to a sunflower disc small molecular peptide extracted from sunflower disc, and also provides its preparation method and its use in the preparation of medicines for preventing and / or treating gout. Background technique [0002] Sunflower flower disc is the flower disc of Helianthus annuuss L., also known as sunflower receptacle, sunflower cake and sunflower house. It is flat in nature, sweet in taste, non-toxic, and returns to the liver meridian. , for the treatment of arthritis, mastitis, dizziness, tinnitus, abdominal pain and so on. The water decoction of sunflower disc has the effect of treating tumors, and the ethanol extract of sunflower disc has a certain antihypertensive effect. It is also used as a medicinal diet for regulating atrophic gastritis. my country is a big sunflower planting country, and the resources of sunflower discs are abundant. Howev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K2/00C12P21/06A61K38/01A61P19/06
CPCA61K38/00C07K2/00C12P21/06
Inventor 不公告发明人
Owner 洮南市金塔生物科技有限公司
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