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Liquid medium dispensing device

A liquid culture medium and liquid shunting technology, which is applied in the field of liquid culture medium sub-packaging devices, can solve the problems of liquid medium waste, small measuring range, low sub-packaging efficiency, etc., and achieves the effect of reducing experiment cost and waste.

Active Publication Date: 2018-06-26
成都英德生物医药设备有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When dispensing liquid medium to cell culture plates, the traditional method is to operate through bottle-top dispensers or pipette guns, but bottle-top dispensers and pipette guns are single-channel dispensing equipment, and the dispensing efficiency is low , long time, high chance of infection
As a result, multi-channel pipettes emerged as the times require, which can quickly and parallelly distribute liquid culture media to cell culture plates. On the other hand, it must be equipped with a sampling tank compatible with the multi-channel pipette tip. In order to ensure that the multi-channel pipette can accurately and effectively absorb the liquid medium, the liquid medium poured into the sampling tank is beyond the actual demand, which will lead to waste of liquid medium and high experiment cost

Method used

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Embodiment Construction

[0017] Such as figure 1 , figure 2 As shown, the liquid medium distributing device includes a bottle-top liquid distributor 3, a connecting pipe 5, a liquid splitter 2 and a support 1; the liquid inlet 21 of the liquid splitter 2 and the liquid outlet 31 of the bottle-top liquid dispenser 3 Connected by the connecting pipe 5, the liquid splitter outlet 22 of the liquid splitter 2 is set downwards, and its number is equal to the number of cell culture wells in the row or column of the cell culture plate. The liquid splitter 2 is supported on the support 1, and the cell culture The plate is placed under the liquid splitter 2, and the cell culture plate can slide horizontally along the direction perpendicular to the connection line of the liquid splitter outlets of the liquid splitter, so that the cell culture wells in different rows or columns of the cell culture plate can be connected with the liquid of the liquid splitter. Corresponding to the shunt outlet.

[0018] The sub...

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PUM

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Abstract

Disclosed is a liquid culture medium subpackaging device.The liquid culture medium subpackaging device comprises a dispensette and is characterized by further comprising a connecting hose, a liquid cyclone and a support, wherein a liquid inlet of the liquid cyclone is communicated with a liquid outlet of the dispensette through the connecting hose, liquid split-flow outlets of the liquid cyclone face downwards and are identical with transversely-arranged or longitudinally-arranged cell culture holes of a cell culture plate in number, the liquid cyclone is supported on the support, the cell culture plate is disposed below the liquid cyclone, and the liquid cyclone or the cell culture plate can slide horizontally along a direction perpendicular to a connecting line of the liquid split-flow outlets of the liquid cyclone, so that different transversely-arranged or longitudinally-arranged cell culture holes of the cell culture plate can correspond to the liquid split-flow outlets of the liquid cyclone.The liquid culture medium subpackaging device is capable of not only subpackaging liquid culture medium into the cell culture plate efficiently and quickly but also reducing waste of the liquid culture medium and experiment cost.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a liquid medium subpackaging device. Background technique [0002] As an important tool in high-throughput culture technology, cell culture plates have been increasingly used in the field of biomedicine. When dispensing liquid medium to cell culture plates, the traditional method is to operate through bottle-top dispensers or pipette guns, but bottle-top dispensers and pipette guns are single-channel dispensing equipment, and the dispensing efficiency is low , The time is long, the probability of infection is high. As a result, multi-channel pipettes emerged as the times require, which can quickly and parallelly distribute liquid culture media to cell culture plates. On the other hand, it must be equipped with a sampling tank compatible with the multi-channel pipette tip. In order to ensure that the multi-channel pipette can accurately and effectively absorb the liquid med...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00
CPCC12M99/00
Inventor 白仲虎孙杨靳魁奇聂简琪隋涌
Owner 成都英德生物医药设备有限公司
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