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A kind of hydrogel, its preparation method and application
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A hydrogel and aqueous solution technology, applied in the field of 3D cell culture, can solve the problems of low controllability and questionable biocompatibility
Active Publication Date: 2019-07-12
SOUTHEAST UNIV
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Embodiment 1
[0026] A method for preparing a hydrogel, comprising the steps of:
[0027] (1) Dissolve 0.1220g of sodium alginate in 18.7mL of deionized water and dissolve overnight at room temperature; dissolve 1.9500g of P(MVE-alt-MAH) in 40mL of deionized water and place in an oil bath at 90°C for 2 hours to obtain a clear Transparent solution; 0.4910g PEG dissolved in 20mL deionized water, dissolved at room temperature;
[0028] (2) Mix the above three aqueous solutions, stir at 800rpm for 10min at room temperature, place in an oven at 80°C for 24h, and obtain a uniform and transparent film;
[0029] n-COOH / n-OH of PMVE-alt-MA and PEG=560:1;
[0037] (4) Flatten the membrane obtained in step (2), soak it in calciumsulfate aqueous solution, and perform ion exchange to obtain P(MVE-alt-MA) / CaAlg hydrogel. The appearance is white and transparent, the storage modulus is 198.34Pa, and the swelling ratio (g / g)=17.06.
[0038] Seeded cells proliferated from single cells to cell aggregates within the hydrogel fragments, and live-cell fluorescent staining also revealed cell viability.
Embodiment 3
[0040] Embodiment 3 is different from embodiment 1 in that:
[0041] (3) 0.4054g of ferric chloride was dissolved in 15mL of deionized water, and dissolved at room temperature to obtain an aqueous solution of ferric chloride;
[0042](4) Flatten the film obtained in step (2), soak it in an aqueous ferric chloride solution, and carry out ion exchange to obtain P(MVE-alt-MA) / FeAlg hydrogel, which is yellow and transparent in appearance and stored in a mold Weight 10480.29Pa, swelling rate (g / g) = 1.89.
[0043] Seeded cells proliferated from single cells to cell aggregates within the hydrogel fragments, and live-cell fluorescent staining also revealed cell viability.
[0044] The processing parameters and raw material consumption of embodiment 4-7 are shown in table 1:
[0045]
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Abstract
The invention discloses hydrogel as well as a preparation method and application thereof. A network structure is formed by poly(methyl vinyl ether-alt-maleic anhydride), PEG and sodium alginate in a cross-linking manner, ion substitution is implemented on sodium ions of the sodium alginate by using metal cation, and thus the hydrogel of a series of cross-linking degrees can be prepared. The preparation method comprises the following steps: (1) preparing solutions of poly(methyl vinyl ether-alt-maleic anhydride), sodium alginate and PEG; (2) mixing the solutions of the three raw materials, and heating to trigger cross-linking reaction so as to obtain a uniform and transparent membrane; (3) substituting sodium ions of the sodium alginate by using metal cation, so as to obtain the hydrogel of different cross-linking degrees. The prepared hydrogel is uniform, transparent, and moderate in strength, and can be used as a 3D culture bracket material for cell culture.
Description
technical field [0001] The invention belongs to the field of 3D cell culture, and in particular relates to a hydrogel, its preparation method and its application. Background technique [0002] Well-defined and highly reproducible hydrogel materials can well mimic the characteristics of extracellular matrix (ECM) by tuning their chemical and physicochemical properties. Hydrogel-based 3D culture systems have the potential to provide in vivo-like cell-extracellular matrix interactions and cell-matrix adhesion. [0003] For the preparation of three-dimensional scaffold materials, natural materials include collagen, hyaluronic acid, matrigel, chitosan, dextran, and alginic acid; synthetic materials are mainly polyethylene glycol (PEG), polylactic acid (PLA) , polylactic-glycolic acid (PLGA) and polyglyceryl sebacate (PGS) and their derivatives. However, among the above-mentioned materials, natural materials with good biocompatibility have low controllability, while synthetic ma...
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