A kind of skeleton polymer for extracting chondroitin sulfate and preparation method thereof
A technology of polymer and skeleton, applied in the field of chemical modification based on melamine sponge skeleton polymer, can solve the problem of small pore size of ion exchange resin, achieve the effect of large freeness, easy electrostatic attraction and improve production efficiency
Active Publication Date: 2018-02-23
南京泛成生物科技有限公司
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Problems solved by technology
[0004] The primary technical problem to be solved by the present invention is to provide a three-dimensional skeleton polymer for the problem of small ion exchange resin pore size in the CS extraction process, which has a three-dimensional network structure and 100 μm through-hole channels, and its skeleton surface has been specially chemically modified , so it is rich in positive charges, and it is easy to adsorb negatively charged CS molecules, avoiding
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[0017] Below in conjunction with embodiment the present invention is described in further detail.
[0018] Material preparation:
[0019] 1. Dissolve chitosan with a viscosity greater than 400mpa.s in dilute acetic acid aqueous solution to a concentration of 1 to 2wt.%, add a small amount of cationic surfactant (dodecyltrimethylammonium chloride, the concentration in the solution 0.1~0.2wt.%), soak the cylindrical melamine sponge (10cm in diameter, 20cm in thickness) in the solution, take it out and suck off the excess solution with a vacuum to ensure that there is no solution flowing out under standing, and then Dry in an oven at 60°C for 2 to 3 hours, and then soak in a sodium carbonate solution with a pH of 9 to 10 for 20 to 30 minutes. After deprotonation of chitosan, an insoluble film is formed to cover the surface of the skeleton;
[0020] 2. The material is washed with water and then soaked in 10-12wt.% ethanol solution of ethylene glycol diglycidyl ether to react at r...
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Abstract
The invention relates to a three-dimensional skeleton polymer and a preparation method thereof. The specific three-dimensional skeleton structure of a melamine sponge is used, and a chitosan coating is cladded on a skeleton; then through a reaction of ethylene glycol diglycidyl ether and the chitosan coating, the ethylene glycol diglycidyl ether and the chitosan coating are in cross-linking, and a large number of epoxy groups are carried on surfaces; then through a reaction of the epoxy groups and diethanolamine, tertiary amine groups are generated; then through an oxidation-reduction reaction of the tertiary amine groups and potassium persulfate at a room temperature, free radicals are generated on the surface of the skeleton, the graft polymerization reaction of the dimethyldiallyl ammonium chloride is further initiated, a large number of cations are introduced on the surface of the skeleton, and the material through cationization is liable to generate electrostatic interaction with chondroitin sulfate macromolecules with negative charges. The material can be used as a whole adsorbing column, and has a pivotal role in the rapid and efficient extraction of the chondroitin sulfate. The material has the advantages that the absorbing capacity is high, the operation is simplified, the material can be repeatedly recycled, the cost is reduced, and the production efficiency is improved.
Description
technical field [0001] The invention relates to the technical field of polymer materials used for extraction of biologically active substances, in particular to a chemical modification method based on a melamine sponge skeleton polymer, and the obtained adsorption material can be used for rapid and efficient extraction of chondroitin sulfate. Background technique [0002] Chondroitin sulfate (chondroitin sulfate, CS) is a sulfated glycosaminoglycan (acid mucopolysaccharide) obtained from animal cartilage tissue. CS is a polyanionic compound with a relative molecular mass of about 10,000 to 35,000. It is a white powder without Odorless, hygroscopic, easily soluble in water to form viscous liquid, insoluble in most organic solvents. As a drug, CS is mainly used for the treatment of migraine, neuralgia, arthritis, prevention and treatment of hearing impairment caused by streptomycin and adjuvant treatment of hepatitis. Prevention and treatment of cardiovascular diseases such a...
Claims
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IPC IPC(8): B01J20/26B01J20/28B01J20/30B01J20/32C08J9/36C08J9/42C08J9/40C08L61/28
Inventor 孙怀艳张瑞丰李伟逊梁云霄江峰肖通虎龙能兵
Owner 南京泛成生物科技有限公司