A blueberry rhizosphere growth-promoting Enterobacter cloacae and its application
An Enterobacter cloacae and rhizosphere growth-promoting technology, applied in the field of plant microorganisms, can solve the problems of lack of PGPR strain resources, difficult to survive and colonize, unable to produce sustained and stable efficacy, etc. Branch length and ground diameter growth, the effect of promoting growth and development
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Embodiment 1
[0023] Screening and Identification of Example 1 Bacterial Strain BIA070
[0024] The screening and isolation process of Enterobacter cloacae BIA070 is as follows:
[0025] (1) Collect soil samples from the blueberry rhizosphere of the blueberry production base in Huangdao District, Qingdao City, which has been planted for 7-8 years. Weigh 10 g of two soil samples respectively and place them in 90 mL of liquid enrichment medium (LB and PDA), 37 ℃, 180rpm shaker culture 24h;
[0026] (2) Spread the enriched bacterial solution on the bromocresol purple color plate by dilution coating method, culture at 37°C for 1-2 days, pick the single colonies that make the surrounding medium change from purple to yellow, and carry out purification;
[0027] (3) Number the purified strains, store them on LB slopes, and put them in a 4°C refrigerator for later use. Inoculate the screened acid-producing strains into test tubes containing bromocresol purple color reagent, shake at 37°C and 180r...
Embodiment 2
[0036] Embodiment 2 reduces soil pH test
[0037] (1) Preparation of BIA070 bacterial agent
[0038] Activate the BIA070 stored on the slant in LB (peptone 10g; NaCl 10g; yeast extract 5g; agar 15-20g; water 1000ml; pH 7.0-7.2, aliquot, sterilize at 121°C for 20min ) on the tablet. Pick a single colony into the corresponding 10ml liquid medium, and culture at 37°C and 180rpm for 24h. The seed solution was inoculated into the corresponding 50ml liquid culture medium according to the inoculum amount of 1% for fermentation, and cultured at 37° C. and 180 rpm for 2 days.
[0039] (2) Reduce soil pH test
[0040] Mix sand, sawdust, and peat in a certain proportion (the same as the blueberry growth conditions), sterilize intermittently at 121°C for 1 hour; under sterile conditions, add 1000g of sterilized substrate to 200ml of bacterial solution, mix well, and dispense to In the 4 Erlenmeyer flasks, seal them with cotton plugs to maintain their air permeability; replenish water ...
Embodiment 3
[0041] Embodiment 3 blueberry nutrition bowl test
[0042] (1) BIA070 bacterial agent is prepared with embodiment 2
[0043] (2) The nutrient pot seedling test was carried out on April 17, 2015 in the nursery of Shandong Academy of Forestry Sciences. The blueberry seedlings were 1-year-old Lanfeng nutrient pot seedlings. After the blueberry container seedlings are planted, take 5ml of the bacterial solution diluted 10 times and inoculate around the roots of the blueberries. No inoculation of bacterial solution (watering the same amount of culture medium) was used as the control group, 10 plants were treated for each, and the main branch length and ground diameter were investigated under normal management. The test results are shown in Table 1. Compared with CK, the main branch length and ground diameter of BIA070 treatment increased by 10.56% and 15.05%, respectively. It can be seen that BIA070 can promote the growth of blueberries.
[0044] Table 1 The results of the bluebe...
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