Hypsizigus marmoreus strain, and culture method, planting method and application thereof

A technology of mushrooms and strains, applied in the field of cultivation, Finc-B-5 strains of mushrooms and their cultivation, can solve the problems of long cultivation period, long production cycle and unsatisfactory taste of mushrooms, and achieve the benefits of The effect of long-distance transportation, high yield per unit area, and high consumer acceptance

Active Publication Date: 2016-07-27
SHANGHAI FINC BIO TECH INC +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a strain of shimeji mushroom, which is used to solve the problems of long production cycle, short fresh-keeping period and unsatisfactory taste of main varieties of shiji mushroom
[0006] The present invention also provides the cultivation and cultivation method of the above-mentioned shiji mushroom strain, in order to solve the problems such as the long cultivation period of shiji mushroom

Method used

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  • Hypsizigus marmoreus strain, and culture method, planting method and application thereof
  • Hypsizigus marmoreus strain, and culture method, planting method and application thereof
  • Hypsizigus marmoreus strain, and culture method, planting method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1 hybridization obtains Finc-B-5

[0051] The strain Finc-B-5 was obtained by conventional cross-breeding method.

[0052] Specific steps are as follows:

[0053]1) Select parent strains: select bacterial strain TNN-11, bacterial strain H-W and bacterial strain Bodo A-264 as parents, and the fruiting bodies of these three parents can be purchased in the market;

[0054] 2) Prepare mother seed culture medium, original seed culture medium and cultivar culture medium: the formulations of each culture medium have been given, and will not be repeated here;

[0055] 3) 600 heterozygotes were obtained by conventional cross-breeding methods;

[0056] 4) Cultivation and screening: The obtained heterozygotes are subjected to cultivation tests according to conventional methods, and through systematic screening, a target bacterial strain is obtained from 600 heterozygotes, and the bacterial strain is named Finc-B-5. Its cultivation and The cultivation cycle is short, ...

Embodiment 2

[0057] Embodiment 2 Antagonism experiment

[0058] Take the mushroom strain Finc-B-5, strain TNN-11, strain H-W, strain Bodo A-264 and strain TNN-12 of the present invention as test materials, and the medium used is PDA medium.

[0059] Strain Finc-B-5, strain TNN-11, strain H-W, strain Bodo A-264, and strain TNN-12 were inoculated and cultured on a flat medium, respectively, with a distance of 3 cm between the inoculation points and cultured at a constant temperature of 23°C for 31 days. See Table 1 for the results.

[0060] Table 1 Antagonism test among different strains of jijiji mushrooms

[0061] Variety

TNN-11

H-W

Bodo A-264

TNN-12

Finc-B-5

+

+

+

+

[0062] Note: "+" indicates antagonism.

[0063] Such as Figure 1-8 As shown, on the front and back of the junction of confrontation culture colonies, strain Finc-B-5 has obvious antagonistic lines with strain TNN-11, strain H-W, strain Bodo A-264 and strain TNN-12,...

Embodiment 3

[0064] Embodiment 3 cultivation experiment

[0065] The mushroom strain Finc-B-5 of the present invention, the most widely planted bacterial strain TNN-12 among the existing mushroom varieties and the shortest cultivation cycle Finc-B-1 of the mushroom that we can obtain (disclosed in Chinese Patent In CN103004464A, the bacterial strain was preserved in the China Typical Culture Collection Center on August 13, 2012, and the preservation number is CCTCCM2012308) respectively carried out the optimization experiment of the cultivation cycle and the cultivation cycle, and each bacterial strain cultivated 80 bottles each time. The yield per unit area is compared, and the advantages and characteristics of the mushroom strain Finc-B-5 of the present invention are summarized.

[0066] The cultivation conditions that present embodiment adopts are as follows:

[0067] See cultivar culture medium for culture medium, no more details.

[0068] Cultivation bottle volume: 850cc.

[0069] ...

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Abstract

The invention relates to a hypsizigus marmoreus strain Finc-B-5, and a culture method, a planting method and application thereof. The hypsizigus marmoreus strain is named as Finc-B-5, belongs to hypsizigus marmoreus and is collected in General Microorganism Center of China Microbiological Culture Collection Management Committee, which is located at No.3, No.1 yard, Beichen west road, Chaoyang district, Beijing. The collection number is CGMCC No.11203, and the collection date is August 13 of 2015. Compared with the prior art, the hypsizigus marmoreus strain Finc-B-5 has the advantages that yield per unit is high, culture and planting periods are short, raw material conversion rate and factory production efficiency are improved greatly, energy is saved, and consumption is reduced; preservation period is long, long-distance transportation is facilitated, shelf life is prolonged, and significance in commercial popularization is great; the shape characteristics are excellent, and product subpackage and sales are really benefited; the mouthfeel is good, and customer acceptability is high; in conclusion, the Finc-B-5 has excellent overall performance and broad market prospect.

Description

technical field [0001] The invention belongs to the technical field of edible fungi, and in particular relates to the mushroom Finc-B-5 strain and its cultivation, cultivation method and application. Background technique [0002] Hypsizygus marmoreus (Latin Hypsizygus marmoreus) belongs to Basidiomycotina, Phyllomycetes, Agaricaceae, Agaricaceae, Liphyllum family, and Amygium genus. Because it has a unique crab flavor, it is also called crab-flavored mushroom. At present, there are mainly two types of shimeji mushrooms produced in factories: brown and pure white. [0003] True jiji mushrooms are rich in vitamins and 17 kinds of amino acids, among which the content of lysine and arginine is higher than that of common mushrooms, which can help teenagers improve intelligence, fight cancer and lower cholesterol. In particular, the extract of the fruiting body (the part above the culture medium) has various physiologically active components. Among them, fungal polysaccharides,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G1/04C12R1/645
CPCA01G18/00C12N1/14C12N1/145C12R2001/645
Inventor 刘玉吴慧敏张文宏
Owner SHANGHAI FINC BIO TECH INC
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