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Test paper card for detecting efficient cyhalothrin residue

A technology of high-efficiency cyhalothrin and colloidal gold test paper, which is applied in the directions of measuring devices, instruments, scientific instruments, etc.

Inactive Publication Date: 2016-07-27
贵州勤邦食品安全科学技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the current research results are hapten synthesis technology solutions, and there is no report on the colloidal gold detection test card for lambda-cyhalothrin

Method used

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  • Test paper card for detecting efficient cyhalothrin residue
  • Test paper card for detecting efficient cyhalothrin residue
  • Test paper card for detecting efficient cyhalothrin residue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1 Preparation of lambda-cyhalothrin colloidal gold detection test paper

[0073] The preparation method of this test paper mainly comprises the following steps:

[0074] 1) Prepare a conjugate release pad coated with lambda-cyhalothrin monoclonal antibody-colloidal gold label;

[0075] 2) Prepare a reaction membrane with a detection line coated with lambda-cyhalothrin hapten-carrier protein conjugate and a quality control line coated with goat anti-mouse anti-antibody;

[0076] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the PVC bottom plate into a test paper.

[0077] The following step-by-step detailed description:

[0078] 1. Preparation of lambda-cyhalothrin hapten

[0079] Weigh 0.42g (1mmol) of efficient cyhalothrin, 0.10g (1mmol) of succinic anhydride and 0.05g of aluminum trichloride respectively.

[0080] 1) Dissolve 0.42g of lambda-cyhalothrin in drie...

Embodiment 2

[0119] The detection of lambda-cyhalothrin residue in the sample of embodiment 2

[0120] 1. Sample pretreatment

[0121] Pretreatment method of fresh tobacco leaf samples:

[0122] (1) Cut the sample into pieces smaller than 1cm before testing;

[0123] (2) Weigh 1.0±0.05g of the chopped sample, put in 5ml of methanol until the liquid surface is fully immersed;

[0124] (3) Close the lid and shake for 1 min;

[0125] (4) After standing still, pipette 0.1ml supernatant and add to 900ul0.1MPBS and mix well.

[0126] Dry tobacco leaf sample pretreatment method:

[0127] (1) Pulverize the sample before testing;

[0128] (2) Weigh 0.5±0.05g of crushed sample and add 5ml of methanol;

[0129] (3) Close the lid and shake for 1 min;

[0130] (4) After standing still, pipette 0.1ml supernatant and add to 900ul0.1MPBS and mix well.

[0131] 2. Test with test paper

[0132] Use a straw to absorb the sample solution to be tested and drop 2-3 drops vertically into the sample hole...

Embodiment 3

[0137] Example 3 sample detection example

[0138] 1. Detection limit test

[0139] Take a blank dry tobacco sample, add lambda-cyhalothrin to it to a final concentration of 0.1, 0.5, 1, 1.5, and 3 mg / kg, respectively, take a test paper for detection, and repeat the measurement three times for each sample.

[0140] When using test paper to detect dry tobacco samples, when the added concentration of lambda-cyhalothrin is 0.1, 0.5 mg / kg, two red lines visible to the naked eye are displayed on the test paper, which is negative; when the concentration of lambda-cyhalothrin is When the added concentration is 1, 1.5, 3mg / kg, the quality control line of the test paper is displayed, but the detection line is not displayed, and it is positive; it shows that the detection line of the test paper for lambda-cyhalothrin in the dry tobacco sample is 1.0mg / kg .

[0141] Fresh tobacco samples were taken, and lambda-cyhalothrin was added therein to final concentrations of 0.1, 0.5, 1, 1.5, a...

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Abstract

The invention discloses a test paper card for detecting an efficient cyhalothrin residue and application. The test paper card comprises a sample absorbing pad (1), a compound releasing pad (2), a reaction film (3), a water absorbing pad (4) and a bottom plate (7), the reaction film is provided with a detection line (5) coated with an efficient cyhalothrin hapten-carrier protein conjugate and a quality control line (6) coated with a goat-anti-mouse antiantibody, and the compound releasing pad (2) is coated with an efficient cyhalothrin monoclonal antibody-colloidal gold marker. The invention further provides a method of detecting the efficient cyhalothrin residue with the test paper card. The provided test paper card has the advantages of being easy to operate, high in sensitivity, high in detection speed, low in cost and suitable for screening and monitoring samples in quantity on site.

Description

technical field [0001] The invention relates to a test paper card for detecting residues of efficient cyhalothrin, in particular to a colloidal gold test card for detecting residues of efficient cyhalothrin. Background technique [0002] Lambda-Cyhalothrin, English name Lambda-Cyhalothrin, also known as Aikonin, λ-cyhalothrin, belongs to pyrethroids, a hygienic insecticide with contact effect, chemical name: α-cyano-3 -Phenoxybenzyl-3-(2-chloro-3,3,3-trichlorofluoro-1-propenyl)-2,2-dimethylcyclopropanecarboxylate (Z)-(1R,3R) , S-ester and (Z), (1S,3S), 1:1 mixture of R-ester. It has contact killing effect and is a broad-spectrum insecticide that is extremely effective against environmental sanitation pests. It has the advantages of fast knockdown speed, strong knockdown force, and less dosage. It can eliminate vector pests that spread diseases and control various sanitary pests. Pyrethroids are known as a new breakthrough in insecticide pesticides and the third milestone ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
CPCG01N33/558
Inventor 扶胜冯才伟杨梅邓传玉谢体波陆苇刘红王大敏牛治存庞宏宇
Owner 贵州勤邦食品安全科学技术有限公司