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Application of hqo as a fluorescent probe for monitoring the process of mitophagy in living cells

A technology of mitophagy and fluorescent probes, applied in fluorescence/phosphorescence, material analysis through optical means, measuring devices, etc., can solve problems such as fluorescent probes without autophagy lysosomes

Active Publication Date: 2018-01-26
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently, there are no fluorescent probes that detect both mitochondria and lysosomes, and no fluorescent probes that detect autophagic lysosomes coated with mitochondria

Method used

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  • Application of hqo as a fluorescent probe for monitoring the process of mitophagy in living cells
  • Application of hqo as a fluorescent probe for monitoring the process of mitophagy in living cells
  • Application of hqo as a fluorescent probe for monitoring the process of mitophagy in living cells

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Embodiment 1

[0034] Example 1. Fluorescent probe HQO monitors the process of mitophagy

[0035] 1. Synthesis of HQO

[0036] Such as figure 1 Shown, according to the following steps to synthesize HQO:

[0037]Add 500mg of IR780 (Bailingwei Technology Co., Ltd.) and 140mg of sodium acetate and 5mL of DMF into a 50mL round-bottomed flask and heat to 80°C for 5 hours. Rotate the evaporator to dry the solvent, and then use high-performance liquid chromatography to purify the product (for details, please refer to the literature " Kim, Y.M.; Choi, Y.D.; Weissleder, R.; Tung, C.H., Membrane permeableesterase-activated fluorescent imaging probe. Bioorg Med Chem Lett 2007, 17(18), 5054-5057" in the disclosed method for purification). The yield was 70%. The structure verification data is as follows: 1 H NMR (400MHz, DMSO): δ (ppm) 7.88 (d, J = 13.2Hz, 2H), 7.28 (d, J = 7.2Hz, 2H), 7.14 (d, J = 7.6Hz, 2H), 6.86 ( m,4H),5.44(d,J=13.2Hz,2H),3.69(t,J=7.2Hz,4H),3.12(d,J=5.2,1H),2.47(d,J=11.2,3H) ,1...

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Abstract

The invention discloses application of HQO as a fluorescent probe used for monitoring a mitochondrial autophagy process in live cells. The structural formula of HQO is shown in a formula I described in the specification. The invention in particular provides application of HQO as or in preparation of the fluorescent probe with any one function described in 1)-3): 1) specifically marking mitochondrial autolysosome in the live cells; 2) marking mitochondria and mitochondrial autolysosome in the live cells at the same time; and 3) monitoring the mitochondrial autophagy process in the live cells. The application disclosed by the invention overcomes the defect that two fluorescent dyes need to be used at the same time for respectively dyeing the mitochondria and lysosome to monitor the mitochondrial autophagy process in the live cells in the prior art.

Description

technical field [0001] The invention relates to the application of HQO as a fluorescent probe for monitoring the process of mitophagy in living cells. Background technique [0002] Mitochondria are intracellular power stations and the main site of active oxygen generation. In addition to supplying cellular energy, they are also involved in many other metabolic processes, such as calcium ion storage, communication and cell differentiation, growth, apoptosis and death. An increase in the amount of reactive oxygen species (ROS) in cells leads to oxidative damage to mitochondria, and the accumulation of mitochondrial damage is associated with cellular aging, cancer and neurodegenerative diseases. Therefore, maintaining a healthy number of mitochondria is essential for cell survival. Damaged mitochondria or excess mitochondria will be selectively eliminated through the process of autophagy to maintain the quality and quantity of mitochondria. Lysosomes are acidic organelles (pH...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06G01N21/64
CPCC09K11/06C09K2211/1029G01N21/6486
Inventor 上官棣华柳影周进王林林刘祥军
Owner INST OF CHEM CHINESE ACAD OF SCI
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