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A kind of tissue rapid propagation method of succulent plant rainbow jade and its cultured rainbow jade

A succulent plant and tissue technology, applied in the field of plant tissue culture, can solve the problems of slow forming and long growth period, and achieve the effects of improving the reproduction coefficient, short production cycle and easy availability of materials

Inactive Publication Date: 2018-03-16
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cultivation of rainbow jade usually adopts the cutting method, that is, remove the complete leaves from the stem, place them for 3 days before cutting, and use leaf cuttings for propagation. Although the reproduction coefficient is large, the formation is slow and the growth period is long. At present, there is no such method. Relevant technologies about the rapid propagation of Hongzhiyu

Method used

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  • A kind of tissue rapid propagation method of succulent plant rainbow jade and its cultured rainbow jade
  • A kind of tissue rapid propagation method of succulent plant rainbow jade and its cultured rainbow jade
  • A kind of tissue rapid propagation method of succulent plant rainbow jade and its cultured rainbow jade

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] In this embodiment: the medium for inducing clustered buds is based on MS medium as the basic medium, and 1.0 mg of 6-benzyl adenine, 1.0 mg of indole acetic acid, 30 g of sucrose, 8.0 g of agar, and 5 g of Activated carbon is prepared, and then the pH value is adjusted to 5.82 with NaOH;

[0018] Subculture medium is based on MS medium as basic medium, add 1.0mg 6-benzyl adenine, 1.0mg indole acetic acid, 30g sucrose, 8.0g agar, 5g activated carbon to each liter of basic medium, and adjust with NaOH The pH value is 5.82.

[0019] (1) Selection and disinfection of explants

[0020] Select a healthy rainbow jade plant, wash the soil on the surface of the plant with water, and then rinse it with running water for 15 minutes. On the ultra-clean workbench, use the volume fraction of 75% alcohol to soak the Rainbow Jade plant for 30 seconds, then wash it with sterile water for 3 times, each time for 2 minutes; get the blade of the Rainbow Jade as an explant, The implant i...

Embodiment 2

[0026] In this embodiment: the medium for inducing clustered buds is based on MS medium as the basic medium, and 0.6 mg of 6-benzyl adenine, 0.6 mg of indole acetic acid, 25 g of sucrose, 8.0 g of agar, and 3 g of Activated carbon is prepared, and then the pH value is adjusted to 5.80 with NaOH;

[0027] Subculture medium is based on MS medium as basic medium, add 1.0mg 6-benzyl adenine, 1.0mg indole acetic acid, 30g sucrose, 8.0g agar, 5g activated carbon to each liter of basic medium, and adjust with NaOH The pH value is 5.82.

[0028] (1) Selection and disinfection of explants

[0029] Select a healthy rainbow jade plant, wash the soil on the surface of the plant with water, and then rinse it with running water for 15 minutes. On the ultra-clean workbench, first use the volume fraction of 70% alcohol to soak the Rainbow Jade plant for 30 seconds, then wash with sterile water 3 times, each 2 minutes; get the stem section of the Rainbow Jade as an explant, and The explants...

Embodiment 3

[0035] In the present embodiment: the medium for inducing clustered buds is based on MS medium as the basic medium, and 1.5 mg of 6-benzyl adenine, 1.5 mg of indole acetic acid, 30 g of sucrose, 8.4 g of agar, and 7 g of Activated carbon is used to adjust the pH value to 6.0 with NaOH;

[0036] Subculture medium is based on MS medium as basic medium, add 1.0mg 6-benzyl adenine, 1.0mg indole acetic acid, 30g sucrose, 8.0g agar, 5g activated carbon to each liter of basic medium, and adjust with NaOH The pH value is 5.82.

[0037] (1) Selection and disinfection of explants

[0038] Select a healthy rainbow jade plant, wash the soil on the surface of the plant with water, and then rinse it with running water for 15 minutes. On the ultra-clean workbench, first use the volume fraction of 70% alcohol to soak the Rainbow Jade plant for 30 seconds, then wash it with sterile water 3 times for 2 minutes each time; take the blade of the Rainbow Jade as an explant, and put the The impla...

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Abstract

The invention relates to a rapid propagation method for tissue of succulent sedum rubrotinctum, and sedum rubrotinctum cultured with the method, and belongs to the technical field of plant tissue culture. The main technical scheme adopted by the invention is as follows: selection and disinfection of explant: taking a leaf or a stem of sedum rubrotinctum as the explant, using ethyl alcohol for soaking the expant whose surface is washed cleanly, and using aqueous solution of mercuric chloride for soaking to obtain sterile explant; inoculating an inducted cluster bud culture medium with the sterile explant, and culturing for 2-3 weeks to obtain a complete plant. According to the method, a seedling of sedum rubrotinctum is obtained by tissue culture of the leaf or the stem of sedum rubrotinctum, materials are obtained easily, the disinfection is convenient, the rate of survival is high, the production cycle is short, the propagation coefficient and the propagation speed of sedum rubrotinctum are improved, the strain advantages of sedum rubrotinctum can be kept better, a large amount of seedlings of sedum rubrotinctum can be rapidly propagated, and the production requirement is met.

Description

technical field [0001] The invention relates to a method for rapid propagation of plant tissue, in particular to a method for rapid tissue propagation of succulent plant rainbow jade and the cultured rainbow jade, belonging to the technical field of plant tissue culture. Background technique [0002] Rainbow Jade (Sedum rubrotinctum) is a succulent plant of the Sedum genus in the Sedum family. It is a perennial succulent herb with a relatively fast growth rate. Spring and summer are the main growing seasons. In summer, the high temperature of 35 degrees requires shade. Its plant height is 10-20 cm , multi-branched, swollen and alternate fleshy leaves, cylindrical to oval, 2 cm long, green, bright skin, no white powder, turning reddish brown in sunny conditions, small yellowish red flowers, lovely shape, popular with everyone I like it, and it has high ornamental value. The cultivation of rainbow jade usually adopts the cutting method, that is, remove the complete leaves fro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01H5/00
CPCA01H4/001A01H4/008A01H5/00
Inventor 金华程文琼郭鹏李琳琳邹吉祥张颖
Owner DALIAN NATIONALITIES UNIVERSITY