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Kit for diagnosing human body Helicobacter pylori infection, and method for detecting content of ammonia gas in human body breath

A Helicobacter pylori and kit technology, applied in the direction of material electrochemical variables, can solve problems such as adverse health effects, time-consuming and labor-intensive, low C13 or C14 content, and achieve rapid detection methods, strong specificity, rapid and accurate diagnosis Effect

Inactive Publication Date: 2016-08-17
长沙三相医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The gastroscope sampling method needs to endure the great pain caused by the gastroscope and is time-consuming and laborious; the method of taking C13 or C14-labeled urea may have potential adverse effects on human health. In addition, the content of C13 or C14 in the air is low. The law cannot give every patient the opportunity to be tested

Method used

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  • Kit for diagnosing human body Helicobacter pylori infection, and method for detecting content of ammonia gas in human body breath
  • Kit for diagnosing human body Helicobacter pylori infection, and method for detecting content of ammonia gas in human body breath
  • Kit for diagnosing human body Helicobacter pylori infection, and method for detecting content of ammonia gas in human body breath

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Linear sweep voltammetry test under different scan rates (scan rate from 100mV s -1 to 10V s -1 ) Ammonia standard gas (10%, 90% N 2 ) in the voltammetric response of [C4mim][OTf] in the ionic liquid.

[0051] The specific operation is as follows:

[0052] The current signal of the test is as figure 1 , the ionic liquid used is [C4mim][OTf], with the increase of scan rate (scan rate from 100mV s -1 to 10V s -1 ), the current peak value of ammonia is continuously increasing.

Embodiment 2

[0054] Using the kit of the invention, the human breath sample is tested by an external standard method.

[0055] In the room temperature ionic liquid [C6mim][PF6], the cyclic voltammogram corresponding to different concentrations of ammonia (1-10ppm), with the increase of ammonia concentration, the current value is significantly enhanced, and the sweep rate is 100mVs- 1 .

[0056] The specific operation is as follows:

[0057] 1) Subjects should eat on an empty stomach or three hours in advance.

[0058] 2) After getting the kit, take the next box of unmarked urea granules in warm water and wait for 30 minutes.

[0059] 3) The subject starts to blow air into the gas collection bag, the capacity of the gas collection bag is between 100-500mL, and then connect the gas collection bag to the air inlet pipe in the electrode assembly, so that the gas can completely flow into the electrode system and react with the ionic liquid .

[0060] 4) Test with the linear sweep voltammetr...

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Abstract

The invention discloses a kit for diagnosing human body Helicobacter pylori infection, and a method for detecting the content of ammonia gas in human body breath. The kit comprises an ionic liquid, an electrode assembly and urea particles. The method for detecting the content of ammonia gas in the human body breath comprises the following steps: collecting the human body breath, introducing the collected human body breath to a container, contacting and chemically reacting ammonia gas in the human body breath with the room temperature ionic liquid in the container, detecting an electrochemical signal in the reaction process through a voltammetry technique, and calculating the content of ammonia gas in the human body breath. Ammonia gas capture and sensor construction can be realized through the ionic liquid and the electrode assembly. The kit and the method realizes rapid and accurate determination of the content of ammonia gas in the human body breath in order to realizes rapid, simple, safe, simple, highly-sensitive and strong-specific diagnosis of various stomach diseases initiated by Helicobacter pylori .

Description

technical field [0001] The invention relates to a kit for diagnosing Helicobacter pylori infection in human body, and 1-n-butyl-3-methylimidazole trifluoromethanesulfinate or 1-butyl-3-methylimidazole hexafluoro A method for detecting ammonia in human exhalation by constructing an electrochemical sensor by reacting room temperature ionic liquids such as phosphate with ammonia, and then realizing the prediction of the infection degree of human Helicobacter pylori, belongs to the field of medical technology. Background technique [0002] Helicobacter pylori (Hp for short) is a helical, slightly anaerobic bacteria that requires very harsh growth conditions. In 1983, it was successfully isolated from the gastric mucosal biopsy tissue of patients with chronic active gastritis for the first time. It is the only microbial species known to be able to survive in the human stomach. [0003] Helicobacter pylori infection can cause gastritis, peptic ulcer, lymphoproliferative gastric l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/30
CPCG01N27/48G01N27/30
Inventor 纪效波周文强李丽娟
Owner 长沙三相医疗器械有限公司