Bendazac lysine pharmaceutical composition and medical application thereof
A technology of benidalysine and composition, applied in pharmaceutical composition and its application field in protecting liver, can solve the problems such as the related report of benandalysine and pharmaceutical composition which have not been seen, and achieve outstanding performance. Substantial features, significant progress, effects of increased protection
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0021] Example 1: Compound (I) Separation Preparation and Structure Confirmation
[0022] Sources of reagents: ethanol, petroleum ether, ethyl acetate, n-butanol, and dichloromethane were of analytical grade, purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd. Methanol, of analytical grade, were purchased from Jiangsu Hanbang Chemical Reagent Co., Ltd.
[0023] Separation method: (a) Crush the dry tuber (2kg) of Corydalis Corydalis, extract with 85% ethanol under heat reflux (15L×3 times), combine the extracts, concentrate until no alcohol smell (3L), and then use petroleum ether (3L×3 times) 3 times), ethyl acetate (3L × 3 times) and water-saturated n-butanol (3L × 3 times) were extracted to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (b) step (a) The ethyl acetate extract in (a) was removed with D101 type macroporous resin, first eluted with 30% ethanol for 6 column volumes, then eluted with 85% ethanol for 12 column volu...
Embodiment 2
[0027] Embodiment 2: pharmacological action
[0028] 1. Materials and methods
[0029] 1.1 Materials and Instruments
[0030] Bendaxyl lysine was purchased from China National Institute for the Control of Pharmaceutical and Biological Products. Compound (I) is self-made, and the preparation method is shown in Example 1. ICR female mice weighing (25 ± 5) g, Shanghai Slack Laboratory Animal Co., Ltd.; Coomassie brilliant blue G-250, triglyceride kit, malondialdehyde test kit, glutathione peroxide kit, Alcohol dehydrogenase assay kit Nanjing Jiancheng Bioengineering Research Institute; Neptune Jinzun, Shenzhen Neptune Pharmaceutical Co., Ltd.; 56° Red Star Erguotou Liquor, Beijing Red Star Brewery. Alpha-D2 lyophilizer, Christ Company, Germany; electronic balance, Shanghai Tianping Instrument Factory; UV752N ultraviolet-visible spectrophotometer, Shanghai Yidian Analytical Instrument Co., Ltd.
[0031] 1.2 Animal grouping
[0032] 72 ICR mice were adaptively fed for one week...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More 