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Preparation method of isoquercitrin and application of isoquercitrin to aspect of resisting asthma

A technology of quercetin and compounds, applied in the field of biopharmaceuticals, can solve the problems of quercetin's low water solubility, poor bioavailability, and limited clinical application

Inactive Publication Date: 2016-08-31
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, quercetin has low water solubility and poor bioavailability, which limits its clinical application.

Method used

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  • Preparation method of isoquercitrin and application of isoquercitrin to aspect of resisting asthma
  • Preparation method of isoquercitrin and application of isoquercitrin to aspect of resisting asthma
  • Preparation method of isoquercitrin and application of isoquercitrin to aspect of resisting asthma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Preparation of isoquercitrin

[0055] Bacterial classification NRRL 1086 is inoculated into liquid PDA medium by 4 ℃ of solid slant (medium contains KH in 100 milliliters 20% potato decoction liquids). 2 PO 4 0.3 g; MgSO 4 0.75g; glucose 10g; vitamin B10.01g, the liquid medium was divided into 150mL Erlenmeyer flasks, each bottle containing 30mL), and cultured in a shaker for 24 hours (28°C, 180r / m), that is, the seeds liquid; the seed liquid was transferred to the liquid PDA medium (the composition of the medium was the same as before) under aseptic conditions, and continued to cultivate in the shaker for 24 hours. Add 1ml quercetin ethanol solution (20mg / ml) to each bottle to convert 200mg in total, and stop the reaction after culturing for 12 hours. The fermentation broth was extracted 5 times with the same amount of ethyl acetate, the extracts were combined, and the ethyl acetate was recovered; 321 mg of the extract was obtained, and 0.8 g of 100-200 mesh silic...

Embodiment 2

[0058] tablet

[0059] Mix 100 g of isoquercitrin prepared in Example 1 with 50 g of starch and 50 g of dextrin, use an appropriate amount of 30% ethanol as a wetting agent to make a soft material, granulate by conventional methods, add an appropriate amount of magnesium stearate and mix to prepare tablet.

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Abstract

The invention relates to the field of biological medicines and in particular relates to a biological transformation preparation method of isoquercitrin (quercetin-3-O-beta-D-glucopyranoside I). The invention further discloses application of the isoquercitrin to the aspect of resisting asthma. The structural formula is shown in the description.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a biotransformation preparation method of isoquercitrin (quercetin-3-O-β-D-glucopyranoside, I), and the invention also discloses its anti-asthma effect use. Background technique [0002] Asthma is an airway chronic allergic inflammatory disease involving eosinophils, T lymphocytes (Th2) and other inflammatory cells, mast cells, and airway epithelial cells. Asthma is a common and frequently-occurring disease, which has caused many adverse effects on human health. At present, there are about 300 million asthma patients in the world, and about 30 million asthma patients in my country. Nearly 200,000 people die from asthma every year, and a large number of studies have shown that the prevalence of asthma has gradually increased in the past 20 years. The pathogenesis of asthma is complex and has not been fully elucidated yet. In current asthma therapy, glucocorticoids are generall...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C12P19/60A61K31/7048A61P11/06C12R1/645
CPCC07H17/07C12P19/60
Inventor 余伯阳许家琦张剑樊妮王倩倩
Owner CHINA PHARM UNIV