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A strain of Acinetobacter lct-h3

A LCT-H3, bacillus technology, applied in bacteria, microorganisms, microorganisms, etc., can solve the problem that genetic information is difficult to obtain

Inactive Publication Date: 2021-07-23
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Early people's understanding of many environmental microorganisms only came from the study of 16S rRNA, and some important genetic information is difficult to obtain

Method used

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  • A strain of Acinetobacter lct-h3
  • A strain of Acinetobacter lct-h3
  • A strain of Acinetobacter lct-h3

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Implementation example one: strain LCT-H3 culture.

[0024] The strain LCT-H3 is a descending strain isolated from the condensed water in the return cabin of the "Shenzhou 9" spacecraft. The strains were cultured statically or shaken in a 37°C incubator or shaker. The liquid medium was LB (Trptone 10g / L, Yeastextract 5g / L, NaCl 5g / L, pH7.3), and the solid medium was MH flat.

Embodiment 2

[0025] Implementation example two: strain LCT-H3 strain identification and phylogenetic tree construction.

[0026] LCT-H3 was inoculated into LB liquid medium, cultivated overnight at 37°C, 180rpm, centrifuged at 6000rpm for 5min to collect the bacteria, and discarded the supernatant. The genomic DNA of the collected bacteria was extracted using the Genomic DNA Extraction Kit (Code No. 51304, detailed steps refer to the product manual) of Qiagen Company. Using the genomic DNA of LCT-H3 as a template, the 16s rRNA fragment was amplified and sequenced using bacterial 16s rRNA universal sequencing primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-TACGGCTACCTTGTTACGACTT-3') to obtain its bases sequence. Use CLUSTALW (http: / / www.ebi.ac.uk / Tools / msa / clustalw2 / ) for multiple sequence alignment, and use MEGA 5.0 to construct the NJ phylogenetic tree ( figure 1 ).

Embodiment 3

[0027] Implementation example three: Gram staining of bacterial strain LCT-H3.

[0028] The Gram staining kit (Code No. G1060, Solarbio) was used to perform Gram staining on the LCT-H3 cells. Cultivate the bacteria overnight in LB liquid medium, drop the bacteria solution on the center of the glass slide, and warm it slightly on the alcohol lamp to make it dry quickly. Crystal violet and sand yellow staining solution can be used to stain and decolorize the dried bacteria at one time. For detailed steps, please refer to the product manual. After the staining is completed and dried, put it under an optical microscope to observe the staining situation, first find the target field of view with a low-power microscope, and then observe with an oil lens (100×), judge and record the results, and take pictures, see figure 2 , Gram-negative bacilli in a single, scattered arrangement.

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Abstract

The invention relates to a descending bacterial strain Acinetobacter sp.LCT‑H3 (Acinetobacter sp.LCT‑H3) isolated from the return cabin of the "Shenzhou 9" spacecraft. And it can respond to different concentrations of iron ions. The whole genome sequencing analysis shows that the genome size is 3.13 Mbp, including 2760 coding sequences and 74 RNA genes. The COG database comparison has annotated 21 groups of COG functional classifications. It is helpful to study the types, distribution and characteristics of microorganisms in airtight aircraft.

Description

technical field [0001] The invention belongs to the field of biotechnology; more specifically, the invention relates to Acinetobacter LCT-H3, its biological characteristics, and whole genome sequencing. Background technique [0002] With the development of aerospace technology, human exploration activities in outer space are becoming more and more frequent. Microorganisms are found almost everywhere on the ground, most of which are found on the human skin, oral cavity, nasopharynx and gastrointestinal tract. Although the manned spacecraft and its internal items have undergone strict vacuum disinfection, microorganisms such as bacteria and fungi are still easy to breed in the airtight environment of manned spacecraft, and these microorganisms can form biofilms on the surfaces of metals and polymer composite materials in airtight cabins , their growth, reproduction and metabolism will corrode materials, which seriously threatens the long-term on-orbit operation safety of the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/205C12R2001/01
Inventor 刘长庭周宏张学林方向群姜学革郭英华刘岩徐绸潘磊李佳余昳
Owner GENERAL HOSPITAL OF PLA