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Setosphaeria turcica spore production medium and application thereof

A technology of maize leaf spot fungus and culture medium, which is applied in the field of plant pathogenic fungus research, can solve the problems of limited breeding of disease-resistant varieties, selection of resistant resources, and low spore production, and achieves convenient material collection, simple ingredients, and operability strong effect

Active Publication Date: 2016-09-07
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Potato dextrose agar (PDA) medium is often used in the prior art for spore-forming culture of P. maize, but due to the small amount of spore production, in-depth research on research work such as breeding of disease-resistant varieties and screening of resistant resources is limited.

Method used

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  • Setosphaeria turcica spore production medium and application thereof
  • Setosphaeria turcica spore production medium and application thereof
  • Setosphaeria turcica spore production medium and application thereof

Examples

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Effect test

Embodiment 1

[0018] (1) Use clean water to prepare the following formula of spore-producing culture medium for maize leaf spot fungus (proportion 1), and each liter contains the following raw material components: 20 g of mannitol, 3 g of peptone, 40 g of fresh corn leaves, and 30 g of sorghum grains g, agar powder 14 g.

[0019] (2) Grind the sorghum grains with a cooking machine for later use, wash the fresh corn leaves with water, add water 20 times the weight of the corn leaves, boil for 30 minutes, and filter, then add crushed sorghum grains, mannitol, and peptone to the supernatant and agar powder, heated to dissolve, added water to make the volume to 1 L, adjusted the pH value to 7.0, sterilized at 121 °C for 20 min, and cooled to obtain a large amount of spore-producing medium for S. maize.

[0020] (3) Take out the Pseudomonas spp. stored in the refrigerator at 4°C, transfer it to PDA medium (PDA medium: 200 g potato, 20 g glucose, 15 g agar powder, 1 L water) for activation, and t...

Embodiment 2

[0022] (1) Use clean water to prepare the following formula of spore-producing culture medium for maize leaf spot fungus (proportion 2), which contains the following raw material components per liter: 25 g of mannitol, 5 g of peptone, 60 g of fresh corn leaves, and 50 g of sorghum grains g, agar powder 16 g.

[0023] (2) Grind the sorghum grains with a cooking machine for later use, wash the fresh corn leaves with water, add water 20 times the weight of the corn leaves, boil for 40 minutes, and filter, then add crushed sorghum grains, mannitol, and peptone to the supernatant and agar powder, heated to dissolve, added water to make the volume to 1 L, adjusted the pH value to 7.0, sterilized at 121 °C for 20 min, and cooled to obtain a large amount of spore-producing medium for S. maize.

[0024] (3) Take out the Pseudomonas spp. stored in the refrigerator at 4°C, transfer it to PDA medium (PDA medium: 200 g potato, 20 g glucose, 15 g agar powder, 1 L water) for activation, and ...

Embodiment 3

[0026] (1) Use clean water to prepare the following formula of spore-producing medium for S. maize leaf spot (proportion 3), which contains the following raw material components per liter: 20 g of mannitol, 5 g of peptone, 40 g of fresh corn leaves, and 40 g of sorghum grains g, agar powder 15 g.

[0027] (2) Grind the sorghum grains with a cooking machine for later use, wash the fresh corn leaves with water, add water 20 times the weight of the corn leaves, boil for 35 minutes, filter, and add crushed sorghum grains, mannitol, and peptone to the supernatant and agar powder, heated to dissolve, added water to make the volume to 1 L, adjusted the pH value to 7.0, sterilized at 121 °C for 20 min, and cooled to obtain a large amount of spore-producing medium for S. maize.

[0028](3) Take out the Pseudomonas spp. stored in the refrigerator at 4°C, transfer it to PDA medium (PDA medium: 200 g potato, 20 g glucose, 15 g agar powder, 1 L water) for activation, and then put Use a ho...

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Abstract

The invention provides a Setosphaeria turcica spore production medium and an application thereof. Every liter of the Setosphaeria turcica spore production medium comprises 20-25g of mannitol, 3-5g of peptone, 40-60g of fresh corn leaves, 30-50g of sorghum grains and 14-16g of agar powder. Setosphaeria turcica is cultured in the spore production medium at 25DEG C in a dark place for 10-15d, aerial mycelia on the surface layer of Setosphaeria turcica are brushed off with sterile water by using a sterilizing writing brush, and light and dark alternate culture is carried out at 25DEG C for 72h to generate a large amount of spores with consistent form and consistent maturity. Corn variety disease resistance is identified through using the Setosphaeria turcica conidiospores generated through culturing by using the medium in order to evaluate the resistance level of corn variety resources to leaf blight, so the prevention and the control of Setosphaeria turcica can be positively realized.

Description

technical field [0001] The present invention relates to the technical field of research on plant pathogenic fungi, in particular to a kind of maize leaf spot fungus ( Exserohilum turcicum ) sporulation medium and its application. Background technique [0002] Northern Corn Leaf Blight (Northern Corn Leaf Blight), also known as leaf spot, blight, and streak, is an important leaf disease of corn all over the world. Exserohilum turcicum (Pass.) Leonard et Suggs) (also known as Umbilophilus maize). The fungus of maize leaf spot mainly attaches to the sick tissue with hyphae or conidia and overwinters, and spreads to the plants by airflow at the beginning of the next year. At a temperature of 20-25°C, germ tubes, epispores, and invasive filaments grow from both ends of the spores, and directly invade from epidermal cells or between epidermal cells. It can be completed in 6-12 hours, and lesions can be formed in 3-4 days After that, the lesions produce conidia, which spread...

Claims

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Application Information

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IPC IPC(8): C12N3/00A01G7/00C12R1/645
CPCA01G7/00C12N3/00
Inventor 石妞妞杜宜新陈福如阮宏椿杨秀娟甘林代玉立
Owner INST OF PLANT PROTECTION FAAS
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