Application of Pap pen with fast operation speed in cell growing slide immunohistochemical detection
A fast-operating, immunohistochemical technology, used in biological testing, material testing, and preparation of samples for testing, etc., can solve problems such as incompetence for large-scale, avoid liquid flow and diffusion, easy operation, and a large number of samples. Effect
Active Publication Date: 2016-09-14
JIANGSU FANGSHIYUANLVE SCI & TECH CONSULTING CO LTD
View PDF5 Cites 1 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0004] Usually, the immunohistochemical detection of cell slides is to stain a single cell slide one by one. Generally, only one type of antibody, protein and gene detection can be performed on a single sample, which is difficult for large-scale, multi-sample and multi-group testing. Medical Experimental Scientific Research
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0038] An application of a multi-purpose immunohistochemistry pen in cell-climbing immunohistochemical detection. Cells are inoculated in a glass slide-type petri dish to perform cell-climbing, and after the cell culture is completed, immunohistochemical staining and identification are performed; The slide-type petri dish is equipped with a petri dish body 1 and a dish cover 2. The model of the culture dish body is divided into a single-well chamber and a multi-well chamber; the area of each well is larger than the slide, and the bottom 3 of the dish is used directly as a slide , The outer surface of the bottom of the dish body is provided with a rectangular frame trace groove 4 corresponding to the size of the slide glass, and the bottom of the dish body can be pulled out along the rectangular frame trace groove under the action of external force;
[0039] The immunohistochemical staining identification includes the following steps in sequence:
[0040] (1) Fix with ice acetone ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses application of a Pap pen with fast operation speed in cell growing slide immunohistochemical detection. Specifically, cells are inoculated in a glass slide type culture dish to let cells grow on a glass slide, at the end of cell culture, immune cell histochemical staining identification is carried out; the glass slide type culture dish is provided with a culture dish body and a dish cover, the culture dish body falls into two types, i.e. a single-hole chamber and a porous chamber. The area of each hole chamber is larger than the glass slide, the dish bottom is directly used as a glass slide, the outside of the dish bottom is provided with a rectangular frame trace line dent corresponding to the glass slide size, and under the action of an external force, the dish bottom can get off along with the rectangular frame trace line dent. The Pap pen is applicable to various immunohistochemical staining experiments of glass slides, can significantly reduce the dosage of antibodies and reagents, avoid liquid flow and diffusion during staining, and improve the operation speed, and is particularly applicable to large-scale, large sample and multi-group immunohistochemical staining in the experimental study of cell growing slides or cell smears.
Description
[0001] This application is a divisional application of application number: 201410500701.1, application date: 2014.9.26, title: "application of multi-purpose immunohistochemical pen in cell-climbing immunohistochemical detection". Technical field [0002] The invention relates to an application of an immunohistochemical pen in cell-climbing immunohistochemical detection. Background technique [0003] Use specific antibodies to conduct tissue and cell in-situ qualitative, localization or quantitative research on the distribution of certain chemical components in tissue sections and cell slides and their contents. This technique is called immunocytochemistry. [0004] Usually the cell slide immunohistochemical test is to stain a single cell slide one by one. Generally, only a single sample can be tested for antibodies, proteins and genes. It is difficult to be competent for large-scale, multi-sample and multi-group tests. Medical experimental scientific research. Summary of the invent...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N1/31G01N33/53
CPCG01N1/30G01N1/31G01N33/53
Inventor 朱顺星李春笋鄂群
Owner JIANGSU FANGSHIYUANLVE SCI & TECH CONSULTING CO LTD