Endophytic Antagonism of Serratia zf05 in Phoenix Dancong Tea Tree and Its Application in Biocontrol
A technology of Serratia and ZF05, which is applied in agricultural biological control, from the endogenous antagonistic bacteria of Fenghuang Dancong tea tree and its application in biological control, can solve the problem of not much research on Fenghuang Dancong tea, and achieve strong Inhibition effect, good application prospect effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0037] Example 1: Isolation, screening and identification of Serratia sp. ZF05GDMCC No: 60015
[0038] 1. Isolation and screening of strains
[0039] The Serratia sp. used in the present invention was sampled by Hanshan Normal University from the leaves of Fenghuang Dancong tea in Fubin Town, Raoping County, Chaozhou, Guangdong Province. The samples were collected from the leaves of Fenghuang Dancong tea in Guangdong Province. Fubin Town, Raoping County, Chaozhou, the trees are 15, 25 and 50 years old, planted at an altitude of 750m, the tea variety is Phoenix Dancong tea narcissus, organically grown. After the homogenization coating method was used to isolate the endophytic bacteria in the leaves, a batch of well-growing bacterial strains were screened out with different culture temperature, pH value, and medium as enrichment conditions, and one strain was selected as Strain of ZF05.
[0040] Separation steps:
[0041] (1) The separation medium is used: beef extract peptone liquid ...
Embodiment 2
[0056] Example 2: Serratia sp. ZF05GDMCC No: 60015 molecular level identification
[0057] Use bacterial DNA genome rapid extraction kit to extract target bacterial DNA.
[0058] The primers used are bacterial universal primers, and bacterial 16SrDNA universal primer sequence:
[0059] 27F: 5'-AGAGTTTGATCCTGGCTCAG-3'
[0060] 1492R: 5′-GGTTACCTTGTTACGACTT-3′
[0061] The primer 27F, primer 1492R, and 2×TapPCR Master mix used in this application were all purchased from BioBio (Shanghai) Co., Ltd.
[0062] The 20μL PCR amplification system used to extract genomic DNA: primer 27F and primer 1492R each 1.0μL, 2×TapPCRMaster mix 7.0μL, endophytic bacteria DNA mixture 1.0μL, double distilled water 10.0μL.
[0063] PCR amplification procedure: pre-denaturation at 94°C for 5 minutes, denaturation at 94°C for 30s, annealing at 55°C for 30s, extension at 72°C for 40s, 30 cycles from denaturation to extension, extension at 72°C for 7 minutes, and incubation for 4 minutes. The PCR products were subj...
Embodiment 3
[0067] Example 3: Growth factor of Serratia sp. ZF05GDMCC No: 60015
[0068] Table 2: The influence of temperature, pH and NaCl on the growth of strain ZF05
[0069] Temperature(℃)
4
10
15
20
25
30
37
40
growing situation
+
+
++
++
+++
++++
+
pH
4
5
6
7
8
9
growing situation
-
+
++
+++
+
-
NaCl concentration
6％
5％
4％
3％
2％
1％
growing situation
-
+
+
+
++
+++
[0070] Serratia sp. (Serratia sp.) ZF05GDMCC No: 60015 was cultivated in the above manner. The culture conditions of the strain were: the medium was beef extract peptone liquid medium and nutrient agar medium, and the culture conditions: pH 7.0, temperature Cultivate for 20h at 37℃.
[0071] According to Table 2, the most suitable growth factor for strain ZF05 was obtained through the above results. The optimum cultivation temperature for Serratiasp. ZF05 GDMCC No: 60015 as the seed was 37°C, and the optimum growth pH was 7.0.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More