a seed treatment
A seed treatment and treatment agent technology, applied in the field of pesticides, can solve problems such as rot, entry of germs, and dry leaves.
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Embodiment 1
[0019] Embodiment 1. Medicament is to the killing effect of cantaloupe fruit spot pathogen
[0020] Prepare the reagents to be tested in different concentrations, filter and sterilize, and treat the bacteria. After standing for a period of time, take 100 μl of various treatment liquids and put them on the culture medium, and spread them evenly. After culturing in an incubator at 26°C for 48 hours, observe the results to determine the killing effect of the agent on the bacteria (Table 1). When the concentration of peracetic acid is above 80 μg / ml, it has a killing effect on germs. When the concentration of hydrochloric acid is 800μg / ml, the treatment time is more than 5min, and there is a killing effect.
[0021] Table 1 The killing effect of test agents on cantaloupe fruit spot fungus
[0022]
[0023] Note: "+" means bacteria growth, "-" means no bacteria growth
Embodiment 2
[0024] Embodiment 2. Situation of seed germination after medicament treatment
[0025] Soak the cantaloupe seeds in 10 8 Cfu / ml bacteria solution for 12h, air-dried. Prepare the test agent into different concentrations, process the air-dried seeds for 15min and 30min, wash them with sterile water for 3 times, then place the processed Hami melon seeds on the agar medium, and treat three dishes (45 seeds) each time, three times per concentration. Repeated, cultivated in 26 ℃ incubator, measured the germination length of seeds after 3 days, the results are shown in Table 2, the peracetic acid and hydrochloric acid treatment seeds of different concentrations, sowing after washing has no adverse effect on the germination and growth of seeds, and the root length is 2-3 times that of the control, it seems to have the effect of stimulating root growth. When the peracetic acid was 800 μg / ml, the germination rate was 1-2% lower than that of the control, and when the peracetic acid was...
Embodiment 3
[0030] Embodiment 3. The control effect of medicament treatment inoculation seed
[0031] Soak the cantaloupe seeds in 10 8 Cfu / ml bacteria solution for 12h, air-dried. Make the test agent into different concentrations, treat the air-dried seeds for 15min and 30min, rinse with sterile water 3 times, sow in sterilized soil, 15 seeds per pot, repeat 3 times, and place in a greenhouse at 25-35°C Among them, the cotyledon incidence was investigated 10 days after emergence, and the incidence was calculated. The results are shown in Table 3. The incidence of cotyledons treated with 1600 μg / ml of peracetic acid for 30 minutes and 10000 μg / ml of hydrochloric acid for 30 minutes were all 0, and the incidence of cotyledons of other treatments was 0. 0.86-3.64%. Therefore, cantaloupe seeds can be treated with peracetic acid 1600μg / ml to control bacterial fruit spot.
[0032] Table 3 inoculated seeds for the disease control effect of test agent treatment
[0033]
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