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A kind of oxidase and its application

An oxidase and oxidative dye technology, applied in the field of daily chemicals, can solve the problems of low oxidase activity, high dosage of precursors, and difficulty in wide application, and achieves the effect of less dosage, high activity, and reduced dosage

Active Publication Date: 2020-10-02
ENZYMEWORKS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above reports, there are many problems such as the amount of precursor used, the activity of oxidase is not high, and the amount of enzyme used is large, so it is difficult to be widely used.

Method used

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  • A kind of oxidase and its application
  • A kind of oxidase and its application
  • A kind of oxidase and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The preparation of embodiment 1 oxidase

[0017] The 1, 3, 5, 7, and 9 gene fragments in the sequence listing were synthesized by Suzhou Jinweizhi Biotechnology Co., Ltd., respectively, and respectively ligated with pET30a (Novagen) plasmid digestion products, and transformed into competent E.coli BL21 (DE3) The strains were screened to obtain positive clones, inoculated into liquid LB medium containing resistance, cultured at 37°C until OD600 to 0.8, added the inducer IPTG, continued to cultivate for 16 hours, centrifuged to collect the precipitate, added phosphate buffer to suspend, Sonicate for 10 minutes in an ice-water bath, centrifuge to get the supernatant, and freeze to obtain oxidase enzyme powder.

Embodiment 2

[0018] Application of embodiment 2 oxidase in hair dyeing process

[0019] Using 0.2 g of 5,6-dihydroxyindole, 10 μg of oxidase enzyme powder prepared in Example 1, and 0.3 mL of surfactant AEO-9 dissolved in 10 mL of 0.01M pH7.0 triethanolamine-HCl buffer solution , add 1g of human hair, carry out the dyeing reaction at room temperature for 10 minutes, then take out the hair and dry it, use a spectrophotometer (Shanghai Yidian WSF type) to check the color difference (ΔE*ab) of the hair before dyeing, and observe with the naked eye Blackening effect, the specific effect is shown in Table 1.

[0020] Table I

[0021] Enzyme type 1 2 3 4 5 ΔE*ab 21 22 20 23 20 Effect visibly darkened visibly darkened visibly darkened visibly darkened visibly darkened

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Abstract

The invention discloses an oxidase and application thereof. The oxidase is adopted in the process of hair dyeing, due to the high activity of the oxidase, the amount of a used oxidation dye is greatly reduced in the process of hair dyeing, the use amount of enzyme is reduced, the reaction condition is mild, the biocompatibility is high, no hair quality is damaged, the allergic dermatitis is not caused, and no harm is done to the human health.

Description

technical field [0001] The invention is applicable to the field of daily chemicals, and in particular relates to an oxidase and its application in the hair dyeing process. Background technique [0002] In the traditional chemical hair dyeing process, chemical oxidants catalyze the oxidation reaction of precursor molecules for dyeing. The most commonly used chemical hair dyes are hydrogen peroxide and p-phenylenediamine. The oxidation ability of hydrogen peroxide is too strong, and it is easy to cause permanent loss of hair quality; p-phenylenediamine is easy to cause allergic dermatitis, and some scholars believe that it also has carcinogenic effect. In recent years, the dyeing process of phenolic polymerization reaction catalyzed by oxidase, especially laccase has overcome the above problems (Formula 1). Laccase is a class of enzymes containing copper ions that can catalyze the single-electron oxidation of substrates. Because the catalyzed oxidation reaction has the charac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/02C12N15/53A61K8/66A61Q5/10
CPCA61K8/66A61Q5/10C12N9/0004
Inventor 鞠鑫
Owner ENZYMEWORKS