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High-quality shrimp oligopeptide powder preparation method suitable for industrial production

An oligopeptide, high-quality technology, applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., to achieve the effects of small molecular weight, improved human utilization, and high production safety

Inactive Publication Date: 2016-11-16
DALIAN SHENLAN PEPTIDE TECH R & D CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction of prawn oligopeptides at home and abroad is still in the development stage. The main research direction is the function of shrimp oligopeptides reported in the literature. The industrial production of shrimp oligopeptides has not been reported. How to make high-quality shrimp oligopeptides Realizing industrialized production and entering life is a problem that needs to be solved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] When seawater shrimp or seawater shrimp and freshwater shrimp are mixed and fed, high-quality shrimp oligopeptide powder can be obtained through the following steps:

[0029] 1. Wash 200kg fresh shrimp raw materials with pure water to remove sediment and other sundries;

[0030] 2. Put the cleaned shrimp into a high-speed chopping machine and mix for 2.5 minutes;

[0031] 3. Grind the chopped and mixed shrimp into a slurry with a colloidal mill; the total protein content in the shrimp slurry is measured to be 21.33% by Kjeldahl;

[0032] 4. Enzymolyze the shrimp slurry: add pure water to the slurry, the mass ratio of slurry to pure water is 1:5, adjust the system temperature to 45°C, and adjust the pH to 5.5 with 10% hydrochloric acid , add compound protease according to 3.0% of the total protein mass in the shrimp meat slurry, stir and enzymolyze for 5 hours to obtain shrimp meat enzymatic hydrolysis solution, the mass ratio of compound protease is: acid protease 40%,...

Embodiment 2

[0042] When freshwater shrimps are all fed, high-quality shrimp oligopeptide powder can be obtained through the following steps:

[0043] 1. Wash 200kg of fresh shrimp raw materials with pure water to remove sediment and other sundries;

[0044] 2. Put the cleaned shrimp into a high-speed chopping machine and mix for 2 minutes;

[0045] 3. Grind the chopped and mixed shrimp into a slurry with a colloidal mill; the total protein content in the shrimp slurry is measured by Kjeldahl to be 20.41%;

[0046] 4. Enzymolyze the shrimp slurry: add pure water to the slurry, the mass ratio of slurry to pure water is 1:7.5, adjust the system temperature to 48°C, and adjust the pH to 5.5 with 10% hydrochloric acid , add compound protease according to 2.5% of the total protein mass in the shrimp meat slurry, stir and enzymolyze for 4 hours, the mass ratio of compound protease is: acid protease 30%, papain 45%, trypsin 17%, flavor enzyme 8%;

[0047] 5. Heat the enzymatic solution to 85°C ...

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Abstract

The invention discloses a high-quality shrimp oligopeptide powder preparation method suitable for industrial production. The method comprises steps as follows: fresh shrimp flesh is taken and subjected to pulping and enzymolysis; a 100-mesh net type filter is adopted for filtration and centrifugation; a clear liquid is collected and separated by an ultrafiltration membrane, and a permeating liquid is collected and is a shrimp oligopeptide liquid; the shrimp oligopeptide liquid subjected to membrane separation is subjected to electrodialysis for secondary desalination; activated carbon is added, the mixture is stirred and is filtered by a titanium rod after decarbonization, and a shrimp oligopeptide refined liquid is obtained; the shrimp oligopeptide refined liquid is subjected to double-effect concentration, evaporative concentration and spray-drying, and the shrimp oligopeptide powder is obtained. The feasible method is provided for industrial production of shrimp oligopeptides, the luster and the taste of products are improved through decoloration, debittering and deodorization treatment, and the popularization performance of the products is improved.

Description

technical field [0001] The invention relates to the technical field of oligopeptide preparation, in particular to a method for extracting high-quality shrimp oligopeptides from shrimp. Background technique [0002] Shrimp is an arthropod that lives in water. It belongs to arthropod crustaceans. There are many types, including Antarctic red shrimp, freshwater shrimp, river shrimp, grass shrimp, prawn, prawn, lobster, etc. Shrimp is a decapod swimmer in the crustacean subphylum, with nearly 2,000 species, large biological storage, wide distribution, and high nutritional value. Modern medical research has confirmed that shrimp has high nutritional value, which can enhance human immunity and sexual function, tonify kidney and strengthen yang, and resist premature aging. It can also be used as a traditional Chinese medicine to treat symptoms such as kidney deficiency, impotence, chills, tiredness, and soreness of the waist and knees. Moreover, the shrimp oligopeptide after deep...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34C07K1/24C07K1/14
CPCC12P21/06C07K1/14C07K1/24C07K1/34
Inventor 包卫洋詹龙全王祖哲彭聪
Owner DALIAN SHENLAN PEPTIDE TECH R & D CO LTD
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