Method for establishing fritillary bulb medicinal material alkaloids fingerprint

A technology of fingerprints and establishment methods, which is applied in the field of establishment of high-performance liquid chromatography fingerprints of Fritillaria chinensis medicinal materials, can solve the problems of incomplete reflection of the overall characteristics of medicinal materials, difficulty in multi-factor detection limitations, and low content of fritillaria. problem, to achieve the effect of simple identification method, ensuring accuracy and good separation

Active Publication Date: 2016-11-16
GUANGXI WUZHOU PHARMA GRP
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  • Abstract
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  • Application Information

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Problems solved by technology

[0005] Fritillaria, peimin A, peimin B, and siberimine are the main alkaloids in Fritillaria chinensis. Because they lack chromophores and have no UV absorption, they cannot be detected by UV absorption detectors.
Researchers use thin layer scanning method, pre-column derivatization method, mass spectrometry-liquid chromatography method, and gas chromatography to determine the content of such alkaloids in Fritillaria, but it is difficult to obtain ideal results due to the limitations of detection.
In recent years, there have been many studies on the detection of alkaloids in Fritillaria chinensis by using high-performance liquid chromatography. Li Huiting et al. used ELSD-HPLC to determine the conte

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  • Method for establishing fritillary bulb medicinal material alkaloids fingerprint
  • Method for establishing fritillary bulb medicinal material alkaloids fingerprint
  • Method for establishing fritillary bulb medicinal material alkaloids fingerprint

Examples

Experimental program
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[0058] Example 1: Establishing the fingerprint of alkaloids in Fritillaria sibiricus medicinal material

[0059] 1. Instruments, reagents and test samples

[0060] Instrument: high performance liquid chromatograph: Aglient1200, evaporative light diffuser: Alltech 3300;

[0061] Electronic balance: Model: CP214, manufactured by Ohaus Instrument Shanghai Co., Ltd.;

[0062] Reference substance: Peimusin reference substance (China Food and Drug Control Study, batch number: 11892-201201); Peimusu A reference substance (China Food and Drug Control Study, content 98.3%, batch number: 110750-201110); Peimousin B reference substance (China Food and Drug Control Study, content is 99.9%, batch number: 110751-201110); Sibeimine reference substance (China Food and Drug Control Study, content is 99.1%, batch number: 110767-201107).

[0063] Sample: Ping Fritillaria medicinal materials (supplied by the Purchasing Department of Wuzhou Pharmaceutical Co., Ltd., batch numbers: 13082101, 13082102, 13082...

Example Embodiment

[0126] Example 2: Establishment of HPLC characteristic profile of Fritillaria sibiricus:

[0127] Fritillaria medicinal materials are provided by the purchasing department of Guangxi Wuzhou Pharmaceutical (Group) Co., Ltd.

[0128] According to high-performance liquid chromatography ("Chinese Pharmacopoeia" 2010 edition an appendix VID) determination:

[0129] Use octadecylsilane-bonded silica gel as filler, mobile phase A is 0.1% triethylamine (containing 1mmol / L ammonium acetate) aqueous solution, mobile phase B is acetonitrile solution; flow rate is 1.0ml / min, using gradient Elution, the elution procedure is as follows,

[0130] 0~8min, mobile phase A is 70%~65%, mobile phase B is 30%~35%;

[0131] 8-25min, mobile phase A is 65%-55%, mobile phase B is 35%-45%;

[0132] 25~40min, mobile phase A is 55%~5%, mobile phase B is 45%~95%;

[0133] 40~43min, mobile phase A is 5%, mobile phase B is 95%;

[0134] 43~48min, mobile phase A is 5%~70%, mobile phase B is 95%~30%

[0135] 48~58min, mobi...

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Abstract

The present invention provides a method for establishing a fritillary bulb medicinal material alkaloids fingerprint. The method includes preparation of a reference substance, preparation of a sample, and acquisition of a fritillary bulb medicinal material fingerprint by using an evaporative light scattering device-high performance liquid chromatography (ELSD-HPLC). Chromatographic conditions and system suitability test use octadecylsilane bonded silica gel as a filler, 0.1% triethylamine (containing 1mmol / L ammonium acetate) water solution as a mobile phase A and acetonitrile as a mobile phase B for gradient elution. The fingerprint has the advantages of high similarity of fingerprint, good degree of separation, precision, stability and reproducibility; and the detection time is within 60min. The invention provides a novel method for the detection of fritillary bulb medicinal material quality.

Description

technical field [0001] The invention relates to a quality control method of traditional Chinese medicine medicinal materials, in particular to a method for establishing fingerprints of flat fritillaria medicinal materials by high-performance liquid chromatography. Background technique [0002] Fritillaria ussurienis Maxim is the dried bulb of Fritillaria ussurienis Maxim, a perennial herb of the genus Fritillaria in the family Liliaceae, also known as Fritillaria ussurienis Maxim. Standard Issue III (2010). [0003] Flat Fritillaria is mainly produced in the three provinces of Northeast China. It is bitter, sweet, and slightly cold in nature; it belongs to the lung and heart meridians. As early as the Qin and Han Dynasties, "Shen Nong's Materia Medica" listed Fritillaria as a middle grade, which can clear away heat and moisten the lungs, reduce phlegm and relieve cough. [0004] The steroidal alkaloid component is the main active ingredient of Fritillaria chinense medicina...

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 刘冠萍林广莲朱小凤陈露招丽君林双
Owner GUANGXI WUZHOU PHARMA GRP
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