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A kind of method of purifying avelamycin

A technology for the crude extract of avelamycin, which is applied in the field of purification of the antibiotic avelamycin, can solve the problems of complex purification steps and unfavorable industrial production, and achieve the effect of simplifying the purification steps

Active Publication Date: 2020-06-16
濮阳泓天威药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN103012519A obtains the pure products of avelamycin A and B through crystallization, silica gel column chromatography, ODS column elution separation, recrystallization, etc. The purification steps are complicated and require two column chromatography, which is not conducive to industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Add 5 g of 20% avelamycin mycelium into 100 mL of methanol, ultrasonicate for 90 min at room temperature, centrifuge to obtain a supernatant, repeat the above steps three times, and combine the supernatants to obtain an extract, according to extract: water The volume ratio of the mixture was 1:1 for hydration and crystallization, centrifuged, and dried to obtain the crude extract of avelamycin.

[0017] The crude extract of avelamycin was dissolved in methanol, and sodium methoxide was added in batches according to the mass ratio of sodium methoxide and crude extract of avelamycin at 1:12 under ice bath, stirred for 30 minutes, and the crude extract of avelamycin was added. The basic aluminum oxide of 3 times of material weight, concentrating under reduced pressure obtains the crude product of mixing sample;

[0018] The mixed sample was dry-loaded to a basic alumina column, eluted with chloroform and methanol at a volume ratio of 96:4, and the eluents of avelamycin A a...

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Abstract

The invention relates to a method for purifying antibiotic avilamycin. The method comprises the following steps: leaching the avilamycin-containing mycelium with a methanol solvent; and obtaining pure products of avilamycin A and B through the steps such as crystallization, sodium methylate reaction, alkaline aluminum oxide column chromatography and recrystallization. In the invention, the obtained pure products are the pure products of avilamycin A and avilamycin B respectively, the purifying steps are simple, industrialization is easy to implement, the average yield is 42.5% or over, and the purity is 98.5% or over.

Description

technical field [0001] The invention relates to a method for purifying the antibiotic avelamycin, belonging to the fields of biomedicine and feed additives. Background technique [0002] Avilamycin (Avilamycin) has a special structure and is generally fermented by Streptomyces viridoehrongenes. It belongs to the oligosaccharide antibiotic of the saccharomycin family. It mainly inhibits Gram-positive bacteria and is a new type of antibiotic. antimicrobial growth promoters and metabolic regulators. In the livestock and poultry industry, it has attracted more and more attention because of its high safety, no cross-resistance and good and stable growth-promoting effect. Avilamycin is a mixed oligosaccharide antibiotic, which has been found to have various structures such as A, B, C, D, F, G, H, etc., among which the most active one is avilamycin group A Part and B component, the activity of other components is very small, and the content is very low. [0003] The existing pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/04C07H1/06
CPCC07H1/06C07H17/04
Inventor 周超陈文成陶锐孙春领尤敏张胜龙师丽君韩振国刘远声李玄涛郭彦超周志霞陈志孙玉佶高淑玲王慧慧张嫄婧
Owner 濮阳泓天威药业有限公司