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Determination method for tilmicosin residual quantity in florfenicol solution

A technology of tilmicosin and florfenicol, which is applied in the field of determination of tilmicosin residues, achieves the effects of simple detection method, short detection cycle and high sensitivity

Inactive Publication Date: 2016-12-14
RINGPU TIANJIN BIOLOGICAL PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0015] In view of the current domestic detection method has not yet used high performance liquid chromatography to determine the status of the research on tilmicosin residues in florfenicol, so a high performance liquid chromatography method was studied to determine the residues of tilmicosin in florfenicol Quantity is very necessary

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  • Determination method for tilmicosin residual quantity in florfenicol solution
  • Determination method for tilmicosin residual quantity in florfenicol solution
  • Determination method for tilmicosin residual quantity in florfenicol solution

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Embodiment Construction

[0032] Preparation of Florfenicol Solution:

[0033] Sample 1, the Florfenicol solution prepared by the method of Example 1 disclosed in CN105796488A;

[0034] Sample 2, the Florfenicol solution prepared by the method of Example 2 disclosed in CN105125483A.

[0035] The assay method of tilmicosin residue in Florfenicol solution of the present invention comprises the following steps:

[0036] Preparation of standard stock solution: Accurately weigh the standard tilmicosin, dissolve it in acetonitrile, prepare a standard stock solution with the same concentration of 1mg / L, and store it at -18°C (the validity period is 1 month); before use Before the preparation, accurately measure the standard stock solution, dilute it with mobile phase into 20ng / ml, 50ng / ml, 100ng / ml, 200ng / ml and 500ng / ml serial gradient standard working solution, store it in the dark at 4°C, and use it on the same day ; Mobile phase preparation: Measure 130mL of acetonitrile, 70mL of tetrahydrofuran, and 50...

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Abstract

The invention relates to a high performance liquid chromatography method for determining the tilmicosin residual quantity in a florfenicol solution. The method comprises the following steps of sample preparation; sample pretreatment, wherein a uniformly-mixed sample for testing is taken to be mixed with methyl alcohol according to the ratio of 1:1, filtering is conducted, and the mixed solution serves as a sample for testing to be filtered through a filter membrane for HPLC analysis; standard solution preparation; high performance liquid chromatography determination. The determination method is convenient, easy and rapid to operate, high in recovery rate and good in repeatability.

Description

technical field [0001] The invention relates to a method for determining the residual amount of tilmicosin in florfenicol solution. Background technique [0002] Tilmicosin is a semi-synthesized macrolide antibiotic for livestock and poultry with tylosin as the precursor. It has a strong inhibitory effect on bacteria (Gram-positive bacteria and some Gram-negative bacteria), mycoplasma, and spirochetes, especially against Actinobacillus pleuropneumoniae, Pasteurella and mycoplasma in pigs. active. The combination of tilmicosin and florfenicol is currently a commonly used drug for the prevention and control of respiratory diseases, and the effect is very good. With the improvement of people's living standards and the strengthening of drug supervision, breeding factories generally require that veterinary drugs used for animals do not contain other drug contamination. [0003] At present, there are national standards for the detection method of tilmicosin residues, but it is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 付春香张晓鹏夏雪林刘爱玲李旭东
Owner RINGPU TIANJIN BIOLOGICAL PHARMA