A kind of preparation method of Chinese medicine extract
A technology of extracts and traditional Chinese medicines, which is applied in the field of preparation of Chinese medicine extracts, can solve the problems of large dosage and low compliance, and achieve the effect of improving curative effect and reducing dosage
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Embodiment 1
[0031] Embodiment 1 The preparation method of Chinese medicine extract of the present invention
[0032] Prescription: Trichosanthes 30g, Guizhi 9g, Paeoniae Alba 9g, Ginger 9g, Jujube 9g, Licorice 6g.
[0033] Take the various medicinal materials in the proportion of the prescription, add 10 times the amount (720mL) of water, decoct twice, 1.5h each time, combine the decoction, filter, concentrate to a solution concentration of 0.3g crude drug / mL, centrifuge for 30min, and centrifuge at 3000r·min -1 , filtered to obtain the sample solution; the sample solution was separated and purified by HPD-100 macroporous resin column, the purification process is: the maximum sample volume is 2.5g crude drug g -1 , the adsorption flow rate is 2BV·h -1 , the eluent is 70% ethanol, and the elution flow rate is 3BV h -1 , the amount of eluent is 6BV; combine and collect 70% ethanol eluate.
Embodiment 2
[0034] Example 2 Dropping pills prepared from Chinese medicine extracts of the present invention
[0035] Concentrate the traditional Chinese medicine extract prepared in Example 1, dry under reduced pressure, and pulverize to obtain the extract powder of Guilou Guizhi Decoction.
[0036] Take Polyethylene Glycol 4000, Polyethylene Glycol 6000 and Cuilou Guizhi Tang Extract Powder in a ratio of 3:2:1, mix them evenly, heat and melt at 90°C, and drop them into pills according to the following conditions: the dropping speed is 20 drop min -1 , The drop distance is 2cm, and the condensation temperature is 10°C.
Embodiment 3
[0037] Embodiment 3 Process screening of the preparation method of the present invention
[0038] 1 Instruments and Medicines
[0039] U-3000 high performance liquid chromatograph (Diane Corporation, USA); XS105 electronic balance (Mettler-Toledo Instrument Co., Ltd.); TGL-16G desktop centrifuge (Shanghai Anting Technology Instrument Factory); ELX- 800 multifunctional microplate reader (Tecan (Shanghai) Trading Co., Ltd.); inverted microscope (Japan OLYMPUS company).
[0040] Trichosanthes (batch number: 20151213), Radix Paeoniae Alba (batch number: 20151211), purchased from Anhui Dechang Pharmaceutical Pieces Co., Ltd.; Guizhi (batch number: 160403), purchased from Bozhou Yonggang Pieces Factory Co., Ltd.; licorice (batch number: 15060901 ), purchased from Fuzhou Huichun Chinese Herbal Pieces Factory Co., Ltd.; ginger and jujube are commercially available; Liquiritin (batch number: 111610-201106) was purchased from China National Institute of Pharmaceutical and Biological P...
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