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Preparation method of ready-to-use nanometer targeted drug carrier

A targeted and drug-based technology, applied in the field of nanomaterials, can solve the problems of cumbersome steps, time-consuming, inability to meet the requirements of personalized assembly of nanoparticles and immediate use, and achieve simple synthesis steps, mild conditions, and instant assembly efficiency the effect of the appeal

Inactive Publication Date: 2016-12-21
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can modify the required functional molecules on the nanoparticles according to the requirements, but it usually takes a long time and the steps are cumbersome, so it is difficult to meet the requirements of obtaining the required nanoparticles according to different requirements in real work and putting them into use immediately. need
With the rapid development of technology and people's increasing demand for personalized precision treatment, this method can no longer meet the requirements of personalized assembly and instant use of nanoparticles.

Method used

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  • Preparation method of ready-to-use nanometer targeted drug carrier
  • Preparation method of ready-to-use nanometer targeted drug carrier
  • Preparation method of ready-to-use nanometer targeted drug carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Through the strong interaction of SA and BT, the method for modifying folic acid molecules on the surface of magnetic nanocrystal clusters

[0035] 0.1g of magnetic nanocrystal clusters (MSP) with carboxyl groups on the surface, 0.1g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) and 0.1g of N-hydroxy Succinimide (NHS) was dispersed in 100 ml of phosphate buffer solution, and 0.2 g of streptavidin was added after activation for two hours. After stirring and reacting for 24 hours, the unreacted SA and small molecule reactants were removed by magnetic separation, and the magnetic separation was repeated 5 times, followed by vacuum drying at 40°C for 2 days to obtain SA surface-modified Magnetic nanocrystal clusters.

[0036] Put 0.1 g of folic acid into water, add 0.05 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) and 0.05 g of N-hydroxysuccinimide (NHS), at 25 o Activation at C for 2h; then add 0.2g ...

Embodiment 2

[0038] Embodiment 2: Through the strong interaction of SA and BT, the method for modifying FITC molecules on the surface of magnetic nanocrystal clusters

[0039] 0.1g of magnetic nanocrystal clusters (MSP) with carboxyl groups on the surface, 0.1g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) and 0.1g of N-hydroxy Succinimide (NHS) was dispersed in 100 ml of phosphate buffer solution, and 0.2 g of streptavidin was added after activation for two hours. After stirring and reacting for 24 hours, the unreacted SA and small molecule reactants were removed by magnetic separation, and the magnetic separation was repeated 5 times, followed by vacuum drying at 60 °C for 1 day to obtain SA surface-modified Magnetic nanocrystal clusters.

[0040] Put 0.1g FITC into water, then add 0.2g biotin hydrazide, stir and react at 25°C for 24 h, and finally obtain FITC-modified biotin.

[0041] Finally, disperse 0.1 g of SA-grafted magnetic nanocrystal clusters obtained...

Embodiment 3

[0042] Embodiment 3: Through the strong interaction of SA and BT, the method for modifying rhodamine molecules on the surface of magnetic nanocrystal clusters

[0043] 0.1g of magnetic nanocrystal clusters (MSP) with carboxyl groups on the surface, 0.1g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) and 0.1g of N-hydroxy Succinimide (NHS) was dispersed in 100 ml of phosphate buffer solution, and 0.2 g of streptavidin was added after activation for two hours. After stirring and reacting for 24 hours, the unreacted SA and small molecule reactants were removed by magnetic separation, and the magnetic separation was repeated 5 times, and then dried at 50°C for 1.5 days by vacuum drying method to obtain SA surface-modified Magnetic nanocrystal clusters.

[0044] Put 0.1 g of rhodamine into water, add 0.05 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) and 0.05 g of N-hydroxysuccinyl Amine (NHS), at 35 o C for 10 h, then add 0...

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Abstract

The invention belongs to the technical field of nanometer materials, and in particular relates to a method for immediately assembling functional molecules on the surfaces of nanometer particles and immediately using the obtained product, aiming at providing a ready-to-use nanometer targeted drug carrier for the individual accurate treatment technology. According to the method, through the strong interaction of streptavidin (SA) and biotin (BT), the corresponding functional molecules are modified on the nanometer particles on which SA is modified through noncovalent bonds. The method specifically comprises the following steps: modifying streptavidin (SA) on the surfaces of the nanometer particles, thus obtaining the nanometer particles with SA on the surface, and grafting the biotin bonded with the different functional molecules to the surfaces of the nanometer particles with modification of SA through the strong interaction between avidin and biotin, thus obtaining the nanometer particles with the surfaces modified by the functional molecules. The method provided by the invention is easy to operate, various functional molecules can be immediately assembled conveniently and efficiently according to different requirements, and the ready-to-use nanometer targeted drug carrier can be immediately put into use, so that the application prospect in the aspect of the individual accurate treatment carrier is good.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a method for modifying functional molecules on the surface of nanoparticles through immediate assembly to meet the needs of instant assembly and instant use (ready-to-use) targeted drug carriers, especially to meet the needs of personalized The need for precise treatment. Background technique [0002] The use of nanomaterials has become very common in our lives. At present, various types of nanoparticles can be conveniently prepared, such as organic polymer nanoparticles, inorganic nanoparticles, organic / inorganic hybrid nanoparticles, etc. Usually, attaching some modified molecules to the surface of nanoparticles will give them some new specific functions. For example, attaching targeting molecules to the surface of drug nanocarrier particles will enable them to target the corresponding tumor cells. Photoacoustic molecules will make it have the ability of phot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K31/704A61P35/00
CPCA61K31/704
Inventor 汪长春孙璐艳
Owner FUDAN UNIV